Diferent from necrosis,apoptosis,autophagy and other forms of cell death,ferroptosis is a mechanism that catalyzes lipid peroxidation of polyunsaturated ftty acids under the action of iron divalent or lipoxygenase,lea...Diferent from necrosis,apoptosis,autophagy and other forms of cell death,ferroptosis is a mechanism that catalyzes lipid peroxidation of polyunsaturated ftty acids under the action of iron divalent or lipoxygenase,leading to cell death.Apatinib is currently used in the third line standard treatment of advanced gastric cancer,targeting the anti-angiogenesis pathway.However,Apatinib mediated ferroptosis in vascular endothelial cells has not been reported yet.Tumor.secreted exosomes can be taken up into target cells to regulate tumor development,but the mechanism related to vascular endothelial cell ferroptosis has not yet been discovered.Here,we show that exosomes secreted by gastric cancer cells carry miR-214.3p into vascular endothelial cells and directdy target zinc finger protein A20 to negatively regulate ACSL4,a key enzyme of lipid peroxidation during frroptosis thereby inhibiting ferroptosis in vascular endothelial cells and reducing the eficiency of Apatinib.In conclusion,inhibition of miR-214-3p can increase the sensitivity of vascular endothelial cells to Apatinib,thereby promoting the antiangiogenic efect of Apatinib,suggesting a potential combination therapy for advanced gastric cancer.展开更多
The development of e cient earth-abundant electrocatalysts for oxygen reduction, oxygen evolution, and hydrogen evolution reactions(ORR, OER, and HER) is important for future energy conversion and energy storage devic...The development of e cient earth-abundant electrocatalysts for oxygen reduction, oxygen evolution, and hydrogen evolution reactions(ORR, OER, and HER) is important for future energy conversion and energy storage devices, for which both rechargeable Zn–air batteries and water splitting have raised great expectations. Herein, we report a single-phase bimetallic nickel cobalt sulfide((Ni,Co)S_2) as an e cient electrocatalyst for both OER and ORR. Owing to the synergistic combination of Ni and Co, the(Ni,Co)S_2 exhibits superior electrocatalytic performance for ORR, OER, and HER in an alkaline electrolyte, and the first principle calculation results indicate that the reaction of an adsorbed O atom with a H_2O molecule to form a *OOH is the potential limiting step in the OER. Importantly, it could be utilized as an advanced air electrode material in Zn–air batteries, which shows an enhanced charge–discharge performance(charging voltage of 1.71 V and discharge voltage of 1.26 V at 2 mA cm^(-2)), large specific capacity(842 mAh g_(Zn)^(-1) at 5 mA cm^(-2)), and excellent cycling stability(480 h). Interestingly, the(Ni,Co)S_2-based Zn–air battery can e ciently power an electrochemical water-splitting unit with(Ni,Co)S_2 serving as both the electrodes. This reveals that the prepared(Ni,Co)S_2 has promising applications in future energy conversion and energy storage devices.展开更多
Littoral-cell angioma(LCA), a primary angioma which clinically belongs to splenic hemangioma, can be mostly found in normal spleen red sinus shore cells of reticuloendothelial cell system. The cells of LCA strongly ex...Littoral-cell angioma(LCA), a primary angioma which clinically belongs to splenic hemangioma, can be mostly found in normal spleen red sinus shore cells of reticuloendothelial cell system. The cells of LCA strongly express endothelial and tissue cell associated antigens that indicate a dual differentiation characteristic; whereas only endothelial cell markers are positive in normal spleen red sinus shore cells. Diagnosis of LCA relies on histopathology. Regular follow-up is needed to monitor recurrence and metastasis.展开更多
A bifunctional heterogeneous catalyst was designed and synthesized,denoted DMEDA/IL–NH2-MIL-101.The structure and physical-chemical characterization of DMEDA/IL–NH2-MIL-101 and its precursors were characterized by S...A bifunctional heterogeneous catalyst was designed and synthesized,denoted DMEDA/IL–NH2-MIL-101.The structure and physical-chemical characterization of DMEDA/IL–NH2-MIL-101 and its precursors were characterized by SEM,N2 adsorption-desorption,XPS,FT-IR,PXRD,elemental analysis,and TGA techniques.The date showed that the two catalytic components of N,N-dimethylethylenediamine(DMEDA)and 1-butyl-3-methylimidazolium bromide(BmimBr)were chemically immobilized in NH2-MIL-101 nanocages.The amine of DMEDA was grafted onto carrier NH2-MIL-101 by N–Cr coordinate covalent bonds and the ionic liquid of BmimBr(IL(Br-))was anchored in the NH2-MIL-101 nanocages by'ship-in-a-bottle'method,in which the amidogen of NH2-MIL-101 condensed with N,N-carbonyldiimidazole(CDI)firstly,and then alkylated with 1-bromo butane.This novel heterogeneous catalyst with two different active sites can efficiently catalyze the synthesis of N-aryl oxazolidin-2-ones from carbon dioxide(CO2),epoxides,and anilines in one-pot under mild solvent-free conditions.It not only showed good stability and recoverability after five cycles but also exhibited shape selectivity for the substrate due to the synergic catalysis of amine,ionic liquid,and NH2-MIL-101.This novel bifunctional material is a promising solid catalyst for the green synthesis of N-aryl oxazolidin-2-ones.展开更多
Objective:To systematically evaluate the efficacy and safety of lopinavir/ritonavir(LPV/r)in the treatment of COVID-19.Methods:PubMed,Embase,Ovid,CNKI,CBM,Wanfang,and VIP databases were searched to obtain the clinical...Objective:To systematically evaluate the efficacy and safety of lopinavir/ritonavir(LPV/r)in the treatment of COVID-19.Methods:PubMed,Embase,Ovid,CNKI,CBM,Wanfang,and VIP databases were searched to obtain the clinical studies of LPV/r in the treatment of COVID-19 from December 2019 to July 2020.The literatures were screened according to the inclusion and exclusion criteria.Their qualities were evaluated according to the Newcastle-Ottawa Scale(NOS)and RevMan 5.3 software was used for meta-analysis.Results:A total of 688 patients were included in five studies,involving China and France.Compared with patients in the control group,who was only treated with routine treatment,there were no significant differences of the 7-day nucleic acid negative conversion rate and 14-day nucleic acid negative conversion rate in the treatment group.However,the use of LPV/r increased the incidence of adverse reactions in the treatment group compared to the control group.Conclusion:There is no available evidence to support the use of Lopinavir/ritonavir in the treatment of COVID-19.展开更多
GPR54 is highly expressed in the central nervous system and plays a crucial role in pubertal development. However, GRP54 is also expressed in the immune system, implying possible immunoregulatory functions. Here we in...GPR54 is highly expressed in the central nervous system and plays a crucial role in pubertal development. However, GRP54 is also expressed in the immune system, implying possible immunoregulatory functions. Here we investigated the role of GPR54 in T cell and immune tolerance. GPR54 deficiency led to an enlarged thymus, an increased number of thymocytes, and altered thymic micro-architecture starting around puberty, indicating GPR54 function in T-cell development through its regulatory effect on the gonadal system. However, flow cytometry revealed a significant reduction in the peripheral regulatory T cell population and a moderate decrease in CD4 single-positive thymocytes in prepubertal Gpr54^(-/-) mice. These phenotypes were confirmed in chimeric mice with GPR54 deficient bone marrow-derived cells. In addition, we found elevated T cell activation in peripheral and thymic T cells in Gpr54^(-/-) mice. When intact mice were immunized with myelin oligodendrocyte glycoprotein, a more severe experimental autoimmune encephalomyelitis(EAE) developed in the Gpr54^(-/-) mice. Interestingly, aggravated EAE disease was also manifested in castrated and bone marrow chimeric Gpr54^(-/-) mice compared to the respective wild-type control,suggesting a defect in self-tolerance resulting from GPR54 deletion through a mechanism that bypassed sex hormones. These findings demonstrate a novel role for GPR54 in regulating self-tolerant immunity in a sex hormone independent manner.展开更多
In common plasmonic structures,absorption and radiation losses are often mutually restricted and can seriously influence the device performance.The current study presents a compound structure composed of multilayer gr...In common plasmonic structures,absorption and radiation losses are often mutually restricted and can seriously influence the device performance.The current study presents a compound structure composed of multilayer grating stripes and multilayer shallow trenches.A small depth was adopted for the trench configuration to exclude the extra bend loss.These two sections supported Fabry–Perot resonance and cavity modes,respectively,with hybrid modes formed through intercoupling.In addition,the total loss for the entire framework was clearly reduced due to the introduction of the trench geometry,indicating that both absorption and radiation losses were successfully taken into consideration in the compound structure.Significantly,such a low loss realized by the hybridization of surface plasmon polariton modes has rarely been seen before.Moreover,the debatable relationship between the total and partial quality factors was described for the first time based on a hybrid mode analysis to establish a new approach to investigate the different resonance modes.In the detailed calculation process,the relative electric field intensity was first adopted to stipulate the effective areas for the various modes,which is more reasonable than using the common definition that is based on a unit structure.The multilayer trench grating exhibited a relatively low loss without weakening energy localization,which is significant in the design of plasmonic devices.展开更多
We present an efficient and robust method which performs well for both strain limiting and treatment of simultaneous collisions. Our method formulates strain constraints and collision constraints as a serial of linear...We present an efficient and robust method which performs well for both strain limiting and treatment of simultaneous collisions. Our method formulates strain constraints and collision constraints as a serial of linear matrix inequalities(LMIs)and linear polynomial inequalities(LPIs), and solves an optimization problem with standard convex semidefinite programming solvers. When performing strain limiting, our method acts on strain tensors to constrain the singular values of the deformation gradient matrix in a specified interval. Our method can be applied to both triangular surface meshes and tetrahedral volume meshes. Compared with prior strain limiting methods, our method converges much faster and guarantees triangle flipping does not occur when applied to a triangular mesh. When performing treatment of simultaneous collisions, our method eliminates all detected collisions during each iteration,leading to higher efficiency and faster convergence than prior collision treatment methods.展开更多
Osteoporosis(OP)is an aging-associated condition that significantly affects the quality of life in aging humans and has few treatment options.As an important marker,recombinant immunoglobulin superfamily containing le...Osteoporosis(OP)is an aging-associated condition that significantly affects the quality of life in aging humans and has few treatment options.As an important marker,recombinant immunoglobulin superfamily containing leucinerich repeat(ISLR)could be used as a gene therapy for OP.We herein used alkaline phosphatase(ALP)and alizarin red S(ARS)staining to evaluate osteogenic differentiation potential.展开更多
基金grants from the National Science Foundation of China(Nos.82173125,81974374,82072664,82103677)Tianjin Key Medical Discipline(Specialty)Construction Project(TJYXZDXK-009A)The Science&Technology Development Fund of Tianjin Education Commission for Higher Education(2020KJ127).
文摘Diferent from necrosis,apoptosis,autophagy and other forms of cell death,ferroptosis is a mechanism that catalyzes lipid peroxidation of polyunsaturated ftty acids under the action of iron divalent or lipoxygenase,leading to cell death.Apatinib is currently used in the third line standard treatment of advanced gastric cancer,targeting the anti-angiogenesis pathway.However,Apatinib mediated ferroptosis in vascular endothelial cells has not been reported yet.Tumor.secreted exosomes can be taken up into target cells to regulate tumor development,but the mechanism related to vascular endothelial cell ferroptosis has not yet been discovered.Here,we show that exosomes secreted by gastric cancer cells carry miR-214.3p into vascular endothelial cells and directdy target zinc finger protein A20 to negatively regulate ACSL4,a key enzyme of lipid peroxidation during frroptosis thereby inhibiting ferroptosis in vascular endothelial cells and reducing the eficiency of Apatinib.In conclusion,inhibition of miR-214-3p can increase the sensitivity of vascular endothelial cells to Apatinib,thereby promoting the antiangiogenic efect of Apatinib,suggesting a potential combination therapy for advanced gastric cancer.
基金supported by the National Natural Science Foundation of China (Grant Nos. 11474137 and 11674143)Program for Changjiang Scholars and Innovative Research Team in University (IRT 16R35)+1 种基金the Fundamental Research Funds for the Central Universities (Grant Nos. LZUMMM2018017, lzujbky-2018-121)the support of Ministry of Education (MOE2016-T2-2-138,Singapore),for research conducted at the National University of Singapore
文摘The development of e cient earth-abundant electrocatalysts for oxygen reduction, oxygen evolution, and hydrogen evolution reactions(ORR, OER, and HER) is important for future energy conversion and energy storage devices, for which both rechargeable Zn–air batteries and water splitting have raised great expectations. Herein, we report a single-phase bimetallic nickel cobalt sulfide((Ni,Co)S_2) as an e cient electrocatalyst for both OER and ORR. Owing to the synergistic combination of Ni and Co, the(Ni,Co)S_2 exhibits superior electrocatalytic performance for ORR, OER, and HER in an alkaline electrolyte, and the first principle calculation results indicate that the reaction of an adsorbed O atom with a H_2O molecule to form a *OOH is the potential limiting step in the OER. Importantly, it could be utilized as an advanced air electrode material in Zn–air batteries, which shows an enhanced charge–discharge performance(charging voltage of 1.71 V and discharge voltage of 1.26 V at 2 mA cm^(-2)), large specific capacity(842 mAh g_(Zn)^(-1) at 5 mA cm^(-2)), and excellent cycling stability(480 h). Interestingly, the(Ni,Co)S_2-based Zn–air battery can e ciently power an electrochemical water-splitting unit with(Ni,Co)S_2 serving as both the electrodes. This reveals that the prepared(Ni,Co)S_2 has promising applications in future energy conversion and energy storage devices.
文摘Littoral-cell angioma(LCA), a primary angioma which clinically belongs to splenic hemangioma, can be mostly found in normal spleen red sinus shore cells of reticuloendothelial cell system. The cells of LCA strongly express endothelial and tissue cell associated antigens that indicate a dual differentiation characteristic; whereas only endothelial cell markers are positive in normal spleen red sinus shore cells. Diagnosis of LCA relies on histopathology. Regular follow-up is needed to monitor recurrence and metastasis.
基金Support of this work by the National Natural Science Foundation of China(21573016)is gratefully acknowledged.
文摘A bifunctional heterogeneous catalyst was designed and synthesized,denoted DMEDA/IL–NH2-MIL-101.The structure and physical-chemical characterization of DMEDA/IL–NH2-MIL-101 and its precursors were characterized by SEM,N2 adsorption-desorption,XPS,FT-IR,PXRD,elemental analysis,and TGA techniques.The date showed that the two catalytic components of N,N-dimethylethylenediamine(DMEDA)and 1-butyl-3-methylimidazolium bromide(BmimBr)were chemically immobilized in NH2-MIL-101 nanocages.The amine of DMEDA was grafted onto carrier NH2-MIL-101 by N–Cr coordinate covalent bonds and the ionic liquid of BmimBr(IL(Br-))was anchored in the NH2-MIL-101 nanocages by'ship-in-a-bottle'method,in which the amidogen of NH2-MIL-101 condensed with N,N-carbonyldiimidazole(CDI)firstly,and then alkylated with 1-bromo butane.This novel heterogeneous catalyst with two different active sites can efficiently catalyze the synthesis of N-aryl oxazolidin-2-ones from carbon dioxide(CO2),epoxides,and anilines in one-pot under mild solvent-free conditions.It not only showed good stability and recoverability after five cycles but also exhibited shape selectivity for the substrate due to the synergic catalysis of amine,ionic liquid,and NH2-MIL-101.This novel bifunctional material is a promising solid catalyst for the green synthesis of N-aryl oxazolidin-2-ones.
文摘Objective:To systematically evaluate the efficacy and safety of lopinavir/ritonavir(LPV/r)in the treatment of COVID-19.Methods:PubMed,Embase,Ovid,CNKI,CBM,Wanfang,and VIP databases were searched to obtain the clinical studies of LPV/r in the treatment of COVID-19 from December 2019 to July 2020.The literatures were screened according to the inclusion and exclusion criteria.Their qualities were evaluated according to the Newcastle-Ottawa Scale(NOS)and RevMan 5.3 software was used for meta-analysis.Results:A total of 688 patients were included in five studies,involving China and France.Compared with patients in the control group,who was only treated with routine treatment,there were no significant differences of the 7-day nucleic acid negative conversion rate and 14-day nucleic acid negative conversion rate in the treatment group.However,the use of LPV/r increased the incidence of adverse reactions in the treatment group compared to the control group.Conclusion:There is no available evidence to support the use of Lopinavir/ritonavir in the treatment of COVID-19.
基金supported by the National Natural Science Foundation of China(31271468)the Science and Technology Commission of Shanghai Municipality(12ZR1408700)
文摘GPR54 is highly expressed in the central nervous system and plays a crucial role in pubertal development. However, GRP54 is also expressed in the immune system, implying possible immunoregulatory functions. Here we investigated the role of GPR54 in T cell and immune tolerance. GPR54 deficiency led to an enlarged thymus, an increased number of thymocytes, and altered thymic micro-architecture starting around puberty, indicating GPR54 function in T-cell development through its regulatory effect on the gonadal system. However, flow cytometry revealed a significant reduction in the peripheral regulatory T cell population and a moderate decrease in CD4 single-positive thymocytes in prepubertal Gpr54^(-/-) mice. These phenotypes were confirmed in chimeric mice with GPR54 deficient bone marrow-derived cells. In addition, we found elevated T cell activation in peripheral and thymic T cells in Gpr54^(-/-) mice. When intact mice were immunized with myelin oligodendrocyte glycoprotein, a more severe experimental autoimmune encephalomyelitis(EAE) developed in the Gpr54^(-/-) mice. Interestingly, aggravated EAE disease was also manifested in castrated and bone marrow chimeric Gpr54^(-/-) mice compared to the respective wild-type control,suggesting a defect in self-tolerance resulting from GPR54 deletion through a mechanism that bypassed sex hormones. These findings demonstrate a novel role for GPR54 in regulating self-tolerant immunity in a sex hormone independent manner.
基金supported by the National Natural Science Foundation of China(Nos.61675199,U1435210,61705226,61705224,aned 11604329).
文摘In common plasmonic structures,absorption and radiation losses are often mutually restricted and can seriously influence the device performance.The current study presents a compound structure composed of multilayer grating stripes and multilayer shallow trenches.A small depth was adopted for the trench configuration to exclude the extra bend loss.These two sections supported Fabry–Perot resonance and cavity modes,respectively,with hybrid modes formed through intercoupling.In addition,the total loss for the entire framework was clearly reduced due to the introduction of the trench geometry,indicating that both absorption and radiation losses were successfully taken into consideration in the compound structure.Significantly,such a low loss realized by the hybridization of surface plasmon polariton modes has rarely been seen before.Moreover,the debatable relationship between the total and partial quality factors was described for the first time based on a hybrid mode analysis to establish a new approach to investigate the different resonance modes.In the detailed calculation process,the relative electric field intensity was first adopted to stipulate the effective areas for the various modes,which is more reasonable than using the common definition that is based on a unit structure.The multilayer trench grating exhibited a relatively low loss without weakening energy localization,which is significant in the design of plasmonic devices.
基金The authors gratefully thank the National Natural Science Foundation of China (No. 21427813) for financial support of this work. This work is also supported by NFFTBS (No. J1310024).
基金This work is supported by the National Natural Science Foundation of China (Nos. 11474137 and 11674143), and Program for Changjiang Scholars and Innovative Research Team in University (IRT 16R35). J. W. thanks for the support of MOE (MOE2016-T2-2-138) for research conducted at the National University of Singapore.
基金supported in part by the National High-tech R&D Program of China (No. 2013AA013903)National Natural Science Foundation of China (No. 61572423)+3 种基金Zhejiang Provincial NSFC (No. LZ16F020003)the National Key Technology R&D Program of China (No. 2012BAD35B01)the DoctoralFund of Ministry of Education of China (No. 20130101110133)Ruofeng Tong is partly supported by National Natural Science Foundation of China (No. 61572424)
文摘We present an efficient and robust method which performs well for both strain limiting and treatment of simultaneous collisions. Our method formulates strain constraints and collision constraints as a serial of linear matrix inequalities(LMIs)and linear polynomial inequalities(LPIs), and solves an optimization problem with standard convex semidefinite programming solvers. When performing strain limiting, our method acts on strain tensors to constrain the singular values of the deformation gradient matrix in a specified interval. Our method can be applied to both triangular surface meshes and tetrahedral volume meshes. Compared with prior strain limiting methods, our method converges much faster and guarantees triangle flipping does not occur when applied to a triangular mesh. When performing treatment of simultaneous collisions, our method eliminates all detected collisions during each iteration,leading to higher efficiency and faster convergence than prior collision treatment methods.
基金supported by the National Natural Science Foundation of China(No.31790412,31970712)the National Key Research and Development Program of China(No.2021YFF1000603)earmarked fund for CARS36,the Plan 111(B12008).
文摘Osteoporosis(OP)is an aging-associated condition that significantly affects the quality of life in aging humans and has few treatment options.As an important marker,recombinant immunoglobulin superfamily containing leucinerich repeat(ISLR)could be used as a gene therapy for OP.We herein used alkaline phosphatase(ALP)and alizarin red S(ARS)staining to evaluate osteogenic differentiation potential.