MamZ protein plays an essential role in magnetosome maturation process of Magnetospirillum gryphiswaldense MSR-1

Based on analysis of gene structure of mamXY operon in Magnetospirillum gryphiswaldense strain MSR-1,we constructed a mamZ deletion mutant strain(ΔmamZ)and four complemented strains with different mamZ fragment lengths.Various cell phenotypic and physiological parameters were evaluated and compared among the wild-type(WT),mutant,and complemented strains.Cell growth rates were not notably different;however,magnetic response(Cmag)and iron uptake ability were significantly lower inΔmamZ.High-resolution transmission electron microscopy(HR-TEM)showed that magnetosomes inΔmamZ were small and irregular,and rock magnetic measurements suggested that they contained immature particles.In comparison to WT of MSR-1,intracellular iron content ofΔmamZ and the complemented strains cultured with 20mmol/L iron source was similar or slightly higher.The complemented strains were unable to synthesize mature or normal amounts of magnetosomes,apparently because of abnormal expression of the transmembrane domain of MamZ protein.Real-time reverse transcription polymerase chain reaction(RTqPCR)analysis showed that relative transcription levels of mamX and ftsZ-like genes inΔmamZ were higher at 18 h than at 12 h,suggesting that MamXY proteins play cooperative functional roles in the magnetosome maturation process.Transcription level of mms6 was significantly upregulated inΔmamZ(incubated at 12 h)and the complemented strains(incubated at 12 and 18 h),refl ecting possible interaction between MamXY and Mms6 proteins during magnetosome biosynthesis.These findings,taken together,demonstrate the essential role of MamZ in the magnetosome maturation process in MSR-1.

Determination of the heating efficiency of magnetotactic bacteria in alternating magnetic field

Magnetotactic bacteria(MTB)intact cells have been applied in magnetic hyperthermia therapy of tumor,showing great efficiency in heating for tumor cell inhibition.However,the detailed magnetic hyperthermia properties and optimum heat production conditions of MTB cells are still poorly understood due to lack of standard measuring equipment.The specific absorption rate(SAR)of MTB cells is often measured by home-made equipment at a limited frequency and magnetic field amplitude.In this study,we have used a commercial standard system to implement a comprehensive study of the hyperthermic response of Magnetospirillum gryphiswaldense MSR-1 strain under 7 frequencies of 144-764 kHz,and 8 field amplitudes between 10 and 45 kA/m.The measurement results prove that the SAR of MTB cells increases with magnetic field frequency and amplitude within a certain range.In combination with the magnetic measurements,it is determined that the magnetic hyperthermia mechanism of MTB mainly follows the principle of hysteresis loss,and the heat efficiency of MTB cells in alternating magnetic field are mainly aff ected by three parameters of hysteresis loop,saturation magnetisation,saturation remanent magnetisation,and coercivity.Thus when we culture MTB in LA-2 medium containing sodium nitrate as source of nitrogen,the SAR of MTB LA-2 cells with magnetosomes arranged in chains can be as high as 4925.6 W/g(in this work,all SARs are calculated with iron mass)under 764 kHz and 30 kA/m,which is 7.5 times than current commercial magnetic particles within similar size range.

Impact of train speed on the mechanical behaviours of track-bed materials

For the 30,000 km long French conventional railway lines(94% of the whole network),the train speed is currently limited to 220 km/h,whilst the speed is 320 km/h for the 1800 km long high-speed lines.Nowadays,there is a growing need to improve the services by increasing the speed limit for the conventional lines.This paper aims at studying the influence of train speed on the mechanical behaviours of track-bed materials based on field monitoring data.Emphasis is put on the behaviours of interlayer and subgrade soils.The selected experimental site is located in Vierzon,France.Several sensors including accelerometers and soil pressure gauges were installed at different depths.The vertical strains of different layers can be obtained by integrating the records of accelerometers installed at different trackbed depths.The experimentation was carried out using an intercity test train running at different speeds from 60 km/h to 200 km/h.This test train was composed of a locomotive(22.5 Mg/axle) and 7 'Corail'coaches(10.5 Mg/axle).It was observed that when the train speed was raised,the loadings transmitted to the track-bed increased.Moreover,the response of the track-bed materials was amplified by the speed rise at different depths:the vertical dynamic stress was increased by about 10% when the train speed was raised from 60 km/h to 200 km/h for the locomotive loading,and the vertical strains doubled their quasistatic values in the shallow layers.Moreover,the stressestrain paths were estimated using the vertical stress and strain for each train speed.These loading paths allowed the resilient modulus Mrto be determined.It was found that the resilient modulus(M_r) was decreased by about 10% when the train speed was increased from 100 km/h to 200 km/h.However,the damping ratio(D_r) kept stable in the range of speeds explored.

Survival of the magnetotactic bacterium Magnetospirillum gryphiswaldense exposed to Earth’s lower near space

地球临近空间指海平面之上20~100 km高度区域,具有高辐射、低温、干燥、低气压等极端条件,是一类典型的类火星环境.研究生命在临近空间的存活机制有助于深化对火星宜居性和生命探测的认识.本研究利用自主研发的临近空间天体生物学载荷和飞行实验平台,将趋磁细菌Magnetospirillum gryphiswaldense MSR-1带入23 km高度区域开展生物暴露实验.结果发现合成磁小体的趋磁细菌经过7小时16分钟的暴露后仍有部分细胞存活,而不合成磁小体的菌株经过暴露后没有检测到存活细胞;临近空间暴露后存活的样品与实验室对照组相比,其磁小体的数量、尺寸和岩石磁学性质等均发生变化.这些结果表明细胞内的磁小体纳米颗粒有助于趋磁细菌抵御临近空间的高辐射等极端环境,为趋磁细菌的起源和早期演化、生命适应类火星极端环境的机制等提供了新认识.鉴于趋磁细菌具有在类火星环境中生存的潜力,这些感磁微生物可以作为天体生物学研究的重要生物类群.

Use of the Jiaolong manned submersible for accurate mapping of deep-sea topography and geomorphology

High-resolution bathymetric side-scan sonar(BSSS) performs the functions of traditional side-scan sonar, while also providing a depth-sounding function that allows simultaneous measurement of seafloor topography and geomorphology. Submarine microtopography and microgeomorphology detection ability and advanced underwater acoustic digital communication are important technical capabilities of the Jiaolong manned submersible. High resolution BSSS achieved accurate detection of seafloor topography and geomorphology at a depth of 7000 m, and successful mapping of local microtopography and microgeomorphology in the Mariana Trench.