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Changes in various metabolic parameters in blood and milk during experimental Escherichia coli mastitis for primiparous Holstein dairy cows during early lactation
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作者 Kasey M Moyes torben larsen +1 位作者 Peter S?rensen Klaus L Ingvartsen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2015年第1期37-46,共10页
Background: The objective of this study was to characterize the changes in various metabolic parameters in blood and milk during IMI challenge with Escherichia coli (E. coh. for dairy cows during early lactation. Th... Background: The objective of this study was to characterize the changes in various metabolic parameters in blood and milk during IMI challenge with Escherichia coli (E. coh. for dairy cows during early lactation. Thirty, healthy primiparous Holstein cows were infused (h = 0) with -20-40 cfu of live E. coil into one front mammary quarter at -4-6 wk in lactation. Daily feed intake and milk yield were recorded. At -12, 0, 3, 6, 12, 18, 24, 36, 48, 60, 72, 96, 108, 120, 132, 144, 156, 168, 180 and 192 h relative to challenge rectal temperatures were recorded and quarter foremilk was collected for analysis of shedding of E. coil Composite milk samples were collected at -180, -132, -84, -36, -12, 12, 24, 36, 48, 60, 72, 84, 96, 132 and 180 h relative to challenge (h=0) and analyzed for lactate dehydrogenase (LDH), somatic cell count, fat, protein, lactose, citrate, beta-hydroxybutyrate (BHBA), free glucose (fglu), and glucose-6-phosphate (G6P). Blood was collected at -12, 0, 3, 6, 12, 18, 24, 36, 60, 72, 84, 132 and 180 h relative to challenge and analyzed for plasma non-esterified fatty acids (NEFA), BHBA and glucose concentration. A generalized linear mixed model was used to determine the effect of IMI challenge on metabolic responses of cows during early lactation. Results: By 12 h, E. coli was recovered from challenged quarters and shedding continued through 72 h. Rectal temperature peaked by 12 h post-challenge and returned to pre-challenge values by 36 h post-IMI challenge. Daily feed intake and milk yield decreased (P 〈0.05) by 1 and 2 d, respectively, after mastitis challenge. Plasma BHBA decreased (12 h, P 〈0.05) from 0.96± 1.1 at 0 h to 0.57±0.64 mmol/L by ]8 h whereas concentration of plasma NEFA (78 h) and glucose (24 h) were significantly greater, 11 and 27%, respectively, after challenge. In milk fglu, lactose, citrate, fat and protein yield were lower whereas yield of BHBA and G6P were higher after challenge when compared to pre-challenge values. Conclusions: Changes in metabolites in blood and milk were most likely associated with drops in feed intake and milk yield. However, the early rise in plasma NEFA may also signify enhanced adipose tissue lipolysis. Lower concentrations of plasma BHBA may be attributed to an increase transfer into milk after IMI. Decreases in both milk lactose yield and % after challenge may be partly attributed to reduced conversion of fglu to lactose. Rises in G6P yield and concentration in milk after challenge (24 h) may signify increased conversion of fglu to G6P. Results identify changes in various metabolic parameters in blood and milk after IMI challenge with E. coli in dairy cows that may partly explain the partitioning of nutrients and changes in milk components after IMI for cows during early lactation. 展开更多
关键词 COW Early lactation Escherichia coli Metabolism
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