Lactobacillus plantarum HL-15 is able to inhibit the growth of mycotoxin producing fungi.To support the application of that culture,the research aimed to produce dried L.plantarum HL-15 and observe its stability durin...Lactobacillus plantarum HL-15 is able to inhibit the growth of mycotoxin producing fungi.To support the application of that culture,the research aimed to produce dried L.plantarum HL-15 and observe its stability during storage was conducted.Production of dried culture was started by fermentation of L.plantarum HL-15 then centrifuged to get the pellet.The pellet was mixed with filler(rice flour or tapioca)with a ratio 1:1(pellet:filler(10%),v/v)then dried.The temperature of the inlet and outlet of the spray dryer used were 105°C and 65°C,respectively.Dried culture was packaged in aluminum foil and sealed then stored at 4°C.Results showed that viable cells of dried inoculum with rice flour filler was 11.99±0.01 log CFU/g and dried inoculum with tapioca filler was 11.90±0.04 log CFU/g.Spray dried L.Plantarum HL-15 was proved being able to inhibit the growth of Aspergillus niger.During six months storage,there is a decrease in the viability of spray dried L.plantarum HL-15 either with rice flour or tapioca filler.During four months of storage,there was a decrease in the activity of inhibition of spray dried culture of L.plantarum HL-15 both with fillers of rice flour and tapioca to the growth of A.niger.L.plantarum HL-15 spray dried cultures could be stored at 4°C for four months for tapioca and five months for rice flour as filler.L.plantarum HL-15 spray dried cultures could inhibit the growth of A.niger so it could be used as a culture for inhibiting the growth of mycotoxin producing fungi in food.展开更多
Chocolate candy is a snack product that is liked by many people.The presence of mycotoxin producing fungi is a problem in chocolate product.The objective of this research was to observe the characteristic of chocolate...Chocolate candy is a snack product that is liked by many people.The presence of mycotoxin producing fungi is a problem in chocolate product.The objective of this research was to observe the characteristic of chocolate candy which is produced from fermented cocoa bean with Lactobacillus plantarum HL-15 starter culture.The cocoa bean(47.5 kg)is fermented by adding L.plantarum HL-15 culture about 500 mL(10^10 CFU/mL)in the new and old fermentation box and in another new and old fermentation box without adding culture.Chocolate candy processing is done based on standard processing of chocolate candy.The results showed that the addition of L.plantarum HL-15 culture in cocoa bean fermentation and use of the new fermentation box give lower fungi concentration on chocolate candy,reducing 1 log cycle from 1.7×10^3 to<10^2 colony/g,while the addition of L.plantarum HL-15 culture in the old fermentation box is not effect.The average aw value of chocolate candy is 0.64 and pH value is 6.7.Fat content of chocolate candy is about 44.9%-46.2%.展开更多
文摘Lactobacillus plantarum HL-15 is able to inhibit the growth of mycotoxin producing fungi.To support the application of that culture,the research aimed to produce dried L.plantarum HL-15 and observe its stability during storage was conducted.Production of dried culture was started by fermentation of L.plantarum HL-15 then centrifuged to get the pellet.The pellet was mixed with filler(rice flour or tapioca)with a ratio 1:1(pellet:filler(10%),v/v)then dried.The temperature of the inlet and outlet of the spray dryer used were 105°C and 65°C,respectively.Dried culture was packaged in aluminum foil and sealed then stored at 4°C.Results showed that viable cells of dried inoculum with rice flour filler was 11.99±0.01 log CFU/g and dried inoculum with tapioca filler was 11.90±0.04 log CFU/g.Spray dried L.Plantarum HL-15 was proved being able to inhibit the growth of Aspergillus niger.During six months storage,there is a decrease in the viability of spray dried L.plantarum HL-15 either with rice flour or tapioca filler.During four months of storage,there was a decrease in the activity of inhibition of spray dried culture of L.plantarum HL-15 both with fillers of rice flour and tapioca to the growth of A.niger.L.plantarum HL-15 spray dried cultures could be stored at 4°C for four months for tapioca and five months for rice flour as filler.L.plantarum HL-15 spray dried cultures could inhibit the growth of A.niger so it could be used as a culture for inhibiting the growth of mycotoxin producing fungi in food.
文摘Chocolate candy is a snack product that is liked by many people.The presence of mycotoxin producing fungi is a problem in chocolate product.The objective of this research was to observe the characteristic of chocolate candy which is produced from fermented cocoa bean with Lactobacillus plantarum HL-15 starter culture.The cocoa bean(47.5 kg)is fermented by adding L.plantarum HL-15 culture about 500 mL(10^10 CFU/mL)in the new and old fermentation box and in another new and old fermentation box without adding culture.Chocolate candy processing is done based on standard processing of chocolate candy.The results showed that the addition of L.plantarum HL-15 culture in cocoa bean fermentation and use of the new fermentation box give lower fungi concentration on chocolate candy,reducing 1 log cycle from 1.7×10^3 to<10^2 colony/g,while the addition of L.plantarum HL-15 culture in the old fermentation box is not effect.The average aw value of chocolate candy is 0.64 and pH value is 6.7.Fat content of chocolate candy is about 44.9%-46.2%.
