食用菌在我国具有悠久的食用和药用传统,其主要功效成分为多糖。多糖化学结构复杂,分析方法繁多,不同方法的特点各不相同。近年来多种多糖分析方法用于食用菌多糖的研究,并取得了很多新的研究进展,但目前鲜有针对食用菌多糖分析方法的...食用菌在我国具有悠久的食用和药用传统,其主要功效成分为多糖。多糖化学结构复杂,分析方法繁多,不同方法的特点各不相同。近年来多种多糖分析方法用于食用菌多糖的研究,并取得了很多新的研究进展,但目前鲜有针对食用菌多糖分析方法的系统、全面综述。本文系统查阅了Web of Science、PubMed、SciFinder Academic和中国知网等数据库,对近20年有关食用菌多糖提取、分离、纯化和定性、定量分析方法的文献进行总结归纳,并对比分析各种方法的特点,旨在为食用菌多糖的深入研究提供参考。展开更多
Distinguished guests,ladies and gentlemen,I am very happy to meet many of my old friends today.I would also like to thank Chinese Pharmaceutical Association for providing me with such a good opportunity to communicate...Distinguished guests,ladies and gentlemen,I am very happy to meet many of my old friends today.I would also like to thank Chinese Pharmaceutical Association for providing me with such a good opportunity to communicate with Chinese and European friends.The topic of my report today is the quality standard of Chinese crude drugs and the development ideas.Director Ma introduced the relevant situation of the quality standards of Chinese patent medicines.展开更多
Psoriasis,a prevalent inherited skin condition,involves an inflammatory response as a key pathogenic mechanism.The Optimized Yinxieling Formula(OYF),rooted in traditional Chinese medicine,is extensively utilized in cl...Psoriasis,a prevalent inherited skin condition,involves an inflammatory response as a key pathogenic mechanism.The Optimized Yinxieling Formula(OYF),rooted in traditional Chinese medicine,is extensively utilized in clinical settings to treat psoriasis.Although previous studies have demonstrated OYF’s significant anti-inflammatory effects in psoriasis,its potential molecular targets and active components remain unexplored.This study aimed to unveil the anti-psoriasis molecular targets and active components of OYF.Our findings indicated that OYF extract markedly reduced the production of several inflammatory mediators,including IL-23,nitric oxide,TNF-α,and IL-1β,in LPS-induced RAW264.7 cells.We synthesized OYF extract-crosslinked beads to isolate pharmacological targets from RAW264.7 lysates using an affinity purification strategy,known as Target Fishing.The enriched target proteins were subsequently identified via LC-MS/MS,followed by bioinformatics analysis to map the psoriasis-associated pathway-gene network.We identified a total of 76 potential target proteins,which were highly associated with mRNA transcription mechanisms.In particular,pathway-gene network analysis revealed that the IL-23 inflammatory pathway was involved in the anti-psoriasis effect of OYF extract.We further utilized a target protein-based affinity capture strategy,combined with LC-MS and SPR analysis,to globally screen OYF’s active components,focusing on the mRNA transcription regulator,fused in sarcoma(FUS).This process led to the identification of umbelliferone,vanillic acid,protocatechuic acid,gentisic acid,and echinacoside as key compounds targeting FUS to inhibit IL-23 expression.Additionally,we formulated a compound cocktail(CpdC),which significantly reduced psoriasis area and severity index(PASI)scores and the expressions of IL-23 and Ki67 in an imiquimod(IMQ)-induced psoriasis mouse model.Collectively,our study elucidates the primary molecular targets and active components of OYF,offering novel insights for psoriasis treatment.展开更多
In this paper, taking the Shuping landslide in Zigui County in Three Gorges Reservoir Area as an example, and specifically evading some monitoring points possibly affected by the water level fluctuating of the Yangtze...In this paper, taking the Shuping landslide in Zigui County in Three Gorges Reservoir Area as an example, and specifically evading some monitoring points possibly affected by the water level fluctuating of the Yangtze River in Three Gorges reservoir area, we conduct smoothing and equidistance processing of the data by separately using orthogonal polynomials and cubic spline ftmction. And it is based on removal of the data with ab- normal changes by using t-test for monitoring data of deformation and rainfall of Shuping landslide. Furthermore, we carry out quantitative analysis of the correlation and summarize the general rule that the landslide deformation is influenced by rainfall. When rainfall reaches a certain threshold, the correlation coefficient between deformation rate and rainfall turns from negative to positive. And the results are given for a number of clear quantitative analysis. It can provide some reference for monitoring and analyzing similar landslides.展开更多
文摘食用菌在我国具有悠久的食用和药用传统,其主要功效成分为多糖。多糖化学结构复杂,分析方法繁多,不同方法的特点各不相同。近年来多种多糖分析方法用于食用菌多糖的研究,并取得了很多新的研究进展,但目前鲜有针对食用菌多糖分析方法的系统、全面综述。本文系统查阅了Web of Science、PubMed、SciFinder Academic和中国知网等数据库,对近20年有关食用菌多糖提取、分离、纯化和定性、定量分析方法的文献进行总结归纳,并对比分析各种方法的特点,旨在为食用菌多糖的深入研究提供参考。
文摘Distinguished guests,ladies and gentlemen,I am very happy to meet many of my old friends today.I would also like to thank Chinese Pharmaceutical Association for providing me with such a good opportunity to communicate with Chinese and European friends.The topic of my report today is the quality standard of Chinese crude drugs and the development ideas.Director Ma introduced the relevant situation of the quality standards of Chinese patent medicines.
基金supported by the State Key Laboratory of Dampness Syndrome of Chinese Medicine,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine(No.SZ2021ZZ51)the National Key R&D Program of China(No.2022YFC3501601)+1 种基金the National Natural Sciences Foundation of China(Nos.82174008,81973505)Jinan New 20 Policies for Higher Education Funding(No.202228048)and the Fundamental Research Funds for the Central Universities.
文摘Psoriasis,a prevalent inherited skin condition,involves an inflammatory response as a key pathogenic mechanism.The Optimized Yinxieling Formula(OYF),rooted in traditional Chinese medicine,is extensively utilized in clinical settings to treat psoriasis.Although previous studies have demonstrated OYF’s significant anti-inflammatory effects in psoriasis,its potential molecular targets and active components remain unexplored.This study aimed to unveil the anti-psoriasis molecular targets and active components of OYF.Our findings indicated that OYF extract markedly reduced the production of several inflammatory mediators,including IL-23,nitric oxide,TNF-α,and IL-1β,in LPS-induced RAW264.7 cells.We synthesized OYF extract-crosslinked beads to isolate pharmacological targets from RAW264.7 lysates using an affinity purification strategy,known as Target Fishing.The enriched target proteins were subsequently identified via LC-MS/MS,followed by bioinformatics analysis to map the psoriasis-associated pathway-gene network.We identified a total of 76 potential target proteins,which were highly associated with mRNA transcription mechanisms.In particular,pathway-gene network analysis revealed that the IL-23 inflammatory pathway was involved in the anti-psoriasis effect of OYF extract.We further utilized a target protein-based affinity capture strategy,combined with LC-MS and SPR analysis,to globally screen OYF’s active components,focusing on the mRNA transcription regulator,fused in sarcoma(FUS).This process led to the identification of umbelliferone,vanillic acid,protocatechuic acid,gentisic acid,and echinacoside as key compounds targeting FUS to inhibit IL-23 expression.Additionally,we formulated a compound cocktail(CpdC),which significantly reduced psoriasis area and severity index(PASI)scores and the expressions of IL-23 and Ki67 in an imiquimod(IMQ)-induced psoriasis mouse model.Collectively,our study elucidates the primary molecular targets and active components of OYF,offering novel insights for psoriasis treatment.
基金Supported by the National Science and Technology Planning Project(2010DFB23380)
文摘In this paper, taking the Shuping landslide in Zigui County in Three Gorges Reservoir Area as an example, and specifically evading some monitoring points possibly affected by the water level fluctuating of the Yangtze River in Three Gorges reservoir area, we conduct smoothing and equidistance processing of the data by separately using orthogonal polynomials and cubic spline ftmction. And it is based on removal of the data with ab- normal changes by using t-test for monitoring data of deformation and rainfall of Shuping landslide. Furthermore, we carry out quantitative analysis of the correlation and summarize the general rule that the landslide deformation is influenced by rainfall. When rainfall reaches a certain threshold, the correlation coefficient between deformation rate and rainfall turns from negative to positive. And the results are given for a number of clear quantitative analysis. It can provide some reference for monitoring and analyzing similar landslides.
