阿糖胞苷分别联合去甲氧柔红霉素、柔红霉素治疗急性髓细胞白血病对恶性分子表达影响的差异

目的:研究去甲氧柔红霉素+阿糖胞苷(IA)方案以及柔红霉素+阿糖胞苷(DA)方案治疗急性髓细胞白血病对恶性分子表达影响的差异。方法:选择在本院诊断为急性髓细胞白血病的56例患者作为研究对象并随机分为IA组和DA组,接受不同化疗方案后2个周期时测定骨髓组织中增殖基因、凋亡基因、侵袭基因的表达量。结果:化疗两个疗程后,IA组患者骨髓组织中Daxx、CDX2、MCL1、BCL2、SOX4、S100A6、MMP9、N-cadherin、ICAM-1、SDF-1的蛋白表达量均显著低于DA组,SHIP1、Bax、C/EBPα的蛋白表达量均显著高于DA组。结论:IA方案治疗急性髓细胞白血病能够较DA方案更为有效地抑制增殖及侵袭基因的表达、增加凋亡基因的表达。

Correlation of Aurora family and TRPV family gene expression in acute myelogenous leukemia with disease progression

Objective:To study the correlation of Aurora family and TRPV family gene expression in acute myelogenous leukemia with disease progression.Methods: Patients who were diagnosed with acute myelogenous leukemia in Kashgar Prefecture First People's Hospital between January 2014 and September 2017 were selected as the leukemia group of the research, and patients who underwent bone marrow puncture and were without myelogram anomaly in Kashgar Prefecture First People's Hospital during the same time were selected as the control group. The bone marrow tissue was collected to determine the mRNA expression of Aurora family and TRPV family genes, apoptosis genes and metastasis genes.Results: Aurora-A, Aurora-B, Aurora-C, TRPV-5, TRPV-6, GFI-1, Bcl-2, CXCR3, C3AR1,β-arrestin1, MMP2 and MMP9 mRNA expression in bone marrow tissue of leukemia group were significantly higher than those of control group whereas CD40L, Bax, Caspase-9 and Caspase-3 mRNA expression were significantly lower than those of control group. Aurora-A, Aurora-B, Aurora-C, TRPV-5 and TRPV-6 mRNA expression in bone marrow tissue of leukemia group were positively correlated with GFI-1, Bcl-2, CXCR3, C3AR1,β-arrestin1, MMP2 and MMP9 mRNA expression, and negatively correlated with CD40L, Bax, Caspase-9 and Caspase-3 mRNA expression.Conclusion: The high expression of Aurora family and TRPV family genes in acute myelogenous leukemia can promote the proliferation and metastasis of tumor cells during disease progression.

急性髓系白血病中Aurora家族及TRPV家族基因表达量与病情进展的相关性

目的:研究急性髓系白血病中Aurora家族及TRPV家族基因表达量与病情进展的相关性。方法:选择2014年1月~2017年9月期间在喀什地区第一人民医院诊断为急性髓系白血病的患者作为研究的白血病组,同期在喀什地区第一人民医院接受骨髓穿刺术且骨髓象无异常的患者作为对照组。取骨髓组织并测定Aurora家族及TRPV家族基因、凋亡基因、转移基因的mRNA表达量。结果:白血病组骨髓组织中Aurora-A、Aurora-B、Aurora-C、TRPV-5、TRPV-6、GFI-1、Bcl-2、CXCR3、C3AR1、β-arrestin1、MMP2、MMP9的mRNA表达量显著高于对照组,CD40L、Bax、Caspase-9、Caspase-3的mRNA表达量显著低于对照组。白血病组骨髓组织中Aurora-A、Aurora-B、Aurora-C、TRPV-5、TRPV-6的mRNA表达量与GFI-1、Bcl-2、CXCR3、C3AR1、β-arrestin1、MMP2、MMP9的mRNA表达量呈正相关,与CD40L、Bax、Caspase-9、Caspase-3的mRNA表达量呈负相关。结论:急性髓系白血病中Aurora家族及TRPV家族基因的高表达能够促进病程进展过程中肿瘤细胞的增殖和转移。

Different influence of Arac combined with idarubicin and with daunorubicin on malignant molecule expression of acute myelocytic leukemia

Objective:To study the different influence of idarubicin + Arac (IA) therapy and daunorubicin + Arac (DA) therapy on malignant molecule expression of acute myelocytic leukemia. Methods: A total of 56 patients who were diagnosed with acute myelocytic leukemia in Kashgar Prefecture First People's Hospital between January 2014 and September 2017 were selected as the research subjects and randomly divided into IA group and DA group, and the expression levels of proliferation genes, apoptosis genes and invasion genes in bone marrow tissue were determined after they accepted two courses of different chemotherapy regimens. Results:After two courses of chemotherapy, Daxx, CDX2, MCL1, BCL2, SOX4, S100A6, MMP9, N-cadherin, ICAM-1 and SDF-1 protein expression in bone marrow tissue of IA group were significantly lower than those of DA group whereas SHIP1, Bax and C/EBP protein expression were significantly higher than those of DA group.Conclusion:IA solution for acute myelocytic leukemia can be more effective than DA solution to inhibit the expression of proliferation and invasion genes and increase the expression of apoptosis genes.