Background The Alere PBP2a SA Culture Colony Test is an FDA-cleared in vitro immunochromatographic assay for rapid detection of penicillin-binding protein2a(PBP2a)in Staphylococcus aureus.Methods We investigated the p...Background The Alere PBP2a SA Culture Colony Test is an FDA-cleared in vitro immunochromatographic assay for rapid detection of penicillin-binding protein2a(PBP2a)in Staphylococcus aureus.Methods We investigated the performance of the PBP2a SA Culture Colony Test with 78 coagulase-negative Staphylococcus(CoNS)isolates from different body sites,with the Vitek 2 Antimicrobial Susceptibility Test(AST)as a reference standard.Results The CoNS species were 62 S.epidermidis;6 S.lugdenensis;3 S.hominis;2 S.capitis;2 S.haemolyticus;and 1 each of S.simulans,S.auricularis,and S.warneri.Of the 78 CoNS isolates,68 showed concordance in the PBP2a IC assay and Vitek 2 AST.Discordance was seen for 10 S.epidermidis isolates,which showed negative in the PBP2a assay,despite oxacillin-resistance detection using the Vitek 2 AST(66.7%sensitivity and 100%specificity).All non-S.epidermidis CoNS were identified with 100%concordance using the PBP2a IC assay and Vitek 2 AST.Conclusion We demonstrated that,while the PBP2a IC assay has low sensitivity in determining the susceptibility of S.epidermidis to oxacillin,it highly accurately predicted the susceptibility of non-S.epidermidis CoNS to oxacillin.The diagnostic accuracy for non-S.epidermidis CoNS needs further assessment with more isolates to confirm our findings.展开更多
Background:Adenosine triphosphate(ATP)-based monitoring systems can detect the amount of organic matter that remains on hospital surfaces after cleaning.We evaluated an ATP-based monitoring system in assessing con-tam...Background:Adenosine triphosphate(ATP)-based monitoring systems can detect the amount of organic matter that remains on hospital surfaces after cleaning.We evaluated an ATP-based monitoring system in assessing con-tamination of high touched objects in rooms occupied by patients on methicillin resistant S.aureus precautions.Methods:We compared the ATP to standard aerobic cultures as well as to gloved hand culture to predict risk of healthcare-worker hand contamination.Results:More than a third of high touch object surfaces were measured unclean with ATP yet only reflects about 5%chance of contaminating healthcare-workers’hands.Conclusions:Our study emphasizes the shortcomings of using the ATP system even in pathogen specific environ-ment such as surfaces in methicillin resistant S.aureus rooms.展开更多
文摘Background The Alere PBP2a SA Culture Colony Test is an FDA-cleared in vitro immunochromatographic assay for rapid detection of penicillin-binding protein2a(PBP2a)in Staphylococcus aureus.Methods We investigated the performance of the PBP2a SA Culture Colony Test with 78 coagulase-negative Staphylococcus(CoNS)isolates from different body sites,with the Vitek 2 Antimicrobial Susceptibility Test(AST)as a reference standard.Results The CoNS species were 62 S.epidermidis;6 S.lugdenensis;3 S.hominis;2 S.capitis;2 S.haemolyticus;and 1 each of S.simulans,S.auricularis,and S.warneri.Of the 78 CoNS isolates,68 showed concordance in the PBP2a IC assay and Vitek 2 AST.Discordance was seen for 10 S.epidermidis isolates,which showed negative in the PBP2a assay,despite oxacillin-resistance detection using the Vitek 2 AST(66.7%sensitivity and 100%specificity).All non-S.epidermidis CoNS were identified with 100%concordance using the PBP2a IC assay and Vitek 2 AST.Conclusion We demonstrated that,while the PBP2a IC assay has low sensitivity in determining the susceptibility of S.epidermidis to oxacillin,it highly accurately predicted the susceptibility of non-S.epidermidis CoNS to oxacillin.The diagnostic accuracy for non-S.epidermidis CoNS needs further assessment with more isolates to confirm our findings.
文摘Background:Adenosine triphosphate(ATP)-based monitoring systems can detect the amount of organic matter that remains on hospital surfaces after cleaning.We evaluated an ATP-based monitoring system in assessing con-tamination of high touched objects in rooms occupied by patients on methicillin resistant S.aureus precautions.Methods:We compared the ATP to standard aerobic cultures as well as to gloved hand culture to predict risk of healthcare-worker hand contamination.Results:More than a third of high touch object surfaces were measured unclean with ATP yet only reflects about 5%chance of contaminating healthcare-workers’hands.Conclusions:Our study emphasizes the shortcomings of using the ATP system even in pathogen specific environ-ment such as surfaces in methicillin resistant S.aureus rooms.
