AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery ...AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery under general anes-thesia with desflurane were studied. Alpha-glutathione-S-transferase(aGST) and IgG4 antibodies againstCYP2E1 were measured preoperatively and 96 h post-operatively, as well as complete blood count, prothrom-bin time(PT), activated partial thromboplastin time(aPTT), international normalized ratio(INR), aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), g-glutamyl-transpeptidase(gGT), alkaline phosphatase, total serum proteins, albumin and bili-rubin. A separate group of 8 patients who received re-gional anesthesia was also studied for calibration of the methodology used for CYP2E1 IgG4 and aGST measure-ments. Student's t-test and the Mann-Whitney U test were used for comparison of the continuous variables, and Fisher's exact test was used for the categorical variables. All tests were two-tailed, with statistical sig-nificance set as P < 0.05.RESULTS: None of the patients developed postopera-tive liver dysfunction, and all patients were successfully discharged from the hospital. No statistically significant difference was observed regarding liver function tests(SGOT, SGPT, γGT, bilirubin, INR), aGST and CYP2E1 IgG4, before and after exposure to desflurane. After dividing patients into two subgroups based on whether or not they had received general anesthesia in the past, no significant difference in the levels of CYP2E1 IgG4 was observed at baseline or 96 h after desflurane administration(P = 0.099 and P = 0.051, respectively). Alpha-GST baseline levels and levels after the interven-tion also did not differ significantly between these two subgroups(P > 0.1). The mean aGST differences were statistically elevated in men by 2.15 ng/mL compared to women when adjusted for BMI, duration of anesthe-sia, number of times anesthesia was administered pre-viously and length of hospital stay. No significant dif-ference was observed between patients who received desflurane and those who received regional anesthesia at any time point.CONCLUSION: There was no difference in CYP2E1 IgG4 or aGST levels after desflurane exposure; furtherresearch is required to investigate their role in desflu-rane-induced liver injury.展开更多
文摘AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery under general anes-thesia with desflurane were studied. Alpha-glutathione-S-transferase(aGST) and IgG4 antibodies againstCYP2E1 were measured preoperatively and 96 h post-operatively, as well as complete blood count, prothrom-bin time(PT), activated partial thromboplastin time(aPTT), international normalized ratio(INR), aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), g-glutamyl-transpeptidase(gGT), alkaline phosphatase, total serum proteins, albumin and bili-rubin. A separate group of 8 patients who received re-gional anesthesia was also studied for calibration of the methodology used for CYP2E1 IgG4 and aGST measure-ments. Student's t-test and the Mann-Whitney U test were used for comparison of the continuous variables, and Fisher's exact test was used for the categorical variables. All tests were two-tailed, with statistical sig-nificance set as P < 0.05.RESULTS: None of the patients developed postopera-tive liver dysfunction, and all patients were successfully discharged from the hospital. No statistically significant difference was observed regarding liver function tests(SGOT, SGPT, γGT, bilirubin, INR), aGST and CYP2E1 IgG4, before and after exposure to desflurane. After dividing patients into two subgroups based on whether or not they had received general anesthesia in the past, no significant difference in the levels of CYP2E1 IgG4 was observed at baseline or 96 h after desflurane administration(P = 0.099 and P = 0.051, respectively). Alpha-GST baseline levels and levels after the interven-tion also did not differ significantly between these two subgroups(P > 0.1). The mean aGST differences were statistically elevated in men by 2.15 ng/mL compared to women when adjusted for BMI, duration of anesthe-sia, number of times anesthesia was administered pre-viously and length of hospital stay. No significant dif-ference was observed between patients who received desflurane and those who received regional anesthesia at any time point.CONCLUSION: There was no difference in CYP2E1 IgG4 or aGST levels after desflurane exposure; furtherresearch is required to investigate their role in desflu-rane-induced liver injury.
