Deep tumor-penetrating nano-delivery strategy to improve diagnosis and therapy in patient-derived xenograft(PDX)oral cancer model and patient tissue

Nanoprodrugs that are directly assembled by prodrugs attract considerable attention with high anticancer potentials.However,their stability and efficiency of tumor-targeted delivery remain a major challenge in practical biomedical applications.Here,we report a new deep tumor-penetrating nano-delivery strategy to achieve enhanced anti-cancer performance by systematic optimization of a porphyrin-doxorubicin-based nanoprodrug using various PEGylations/crosslinks and co-administration of targeting peptide iRGD.Polyethylene glycols(PEGs)with different molecular weights and grafts are employed to crosslink the nanoprodrug and optimize size,charge,tumor accumulation and penetration,and anti-cancer efficiency,etc.The tumor penetration was validated in syngeneic oral cancer mouse models,patient-derived xenograft(PDX)models,and oral cancer tissue from patients.The optimized nanoprodrug co-administrated with iRGD remarkably enhances the accumulation and penetration both in tumor vascular and PDX tumor tissue.It is effective and safe to improve in vivo therapeutic efficacy via the passive tumor targeting dependent and independent mode.Our tumor-penetrating nano-delivery strategy is promising to strengthen the nanoprodrugs in clinical implementation.

Excipient-free porphyrin/SN-38 based nanotheranostics for drug delivery and cell imaging

Nanotheranostics with comprehensive diagnostic and therapeutic capabilities show exciting cancer treatment potentials.Here,we develop an excipient-free drug delivery system for cancer diagnosis as well as therapy,in which a near infra-red photosensitizer and a chemotherapeutic drug can be self-delivered without any carriers.The building block of the drug delivery system was synthesized by covalently conjugating four anticancer drugs(7-ethyl-10-hydroxy-camptothecin,SN-38)with a photosensitizer(porphyrin)via hydrolyzable ester linkage,which endows the drug delivery system with 100%active pharmaceutical ingredients,excellent imaging,and therapeutic functionalities.The conjugates can readily self-assemble into nanosheets(PS NSs)and remain stable for at least 20 days in aqueous solution.In PS NSs,fluorescence resonance energy transfer(FRET)dominates the fluorescence of SN-38 and enables to monitor the drug release fluorescentiy.The PS NSs also show excellent anticancer activity in vitro,due to the increased cell uptake with the synergistic effect of photodynamic therapy and chemotherapy.