This study was conducted to evaluate the effect of different distillation methods of Mentha cordifolia Opiz. essential oil on antibacterial activity. The essential oils were isolated by water and steam, hydro, ethanol...This study was conducted to evaluate the effect of different distillation methods of Mentha cordifolia Opiz. essential oil on antibacterial activity. The essential oils were isolated by water and steam, hydro, ethanol, and 3methanol： lethanol distillations. Moreover, we also compared the efficacy of these various distillations with commercial peppermint oil. Essential oils were tested in vitro against three pathogen bacteria species by sensitivity test include disc diffusion assay （DD）, minimal inhibition concentration （MIC） and maximal bactericidal concentration （MBC）. Escherichia coli, Staphylococcus aureus and Salmonella typhimurium were used in this investigation. The results showed that the water and steam distillation and 3methanol： lethanol under tested by DD assay was found to be effective against all the pathogenic bacteria, in which the zone of inhibition exhibited E. coil S. aureus and S. typhimurium were 9.67 ± 0.35, 11.67 ± 0.66, 18.0± 1.15 and 9.00 ± 0.05, 8.3 ± 0.35, 19.0 ± 0.00 ram, respectively. While the hydro and ethanol distillations did action to against only E. coli which the inhibition zones were 8.67 ± 0.66 and 8.67 ± 0.35 mm, respectively. However, the commercial oil was more effective against tested pathogenic bacteria than all M. cordifolia essential oil. In case of MIC and MBC assays, the results showed that all essential oil distillation methods posed antibacterial potential in which the water and steam distillation showed the lowest MIC and MBC values against E. coli, S. aureus and S. typhimurium. It is suggested that among all M. cordifolia essential oil extractions, water and steam distillation was found to be highly bactericidal as it has shown in lowest MIC and MBC values and high in growth inhibition zone diameter.展开更多
文摘This study was conducted to evaluate the effect of different distillation methods of Mentha cordifolia Opiz. essential oil on antibacterial activity. The essential oils were isolated by water and steam, hydro, ethanol, and 3methanol： lethanol distillations. Moreover, we also compared the efficacy of these various distillations with commercial peppermint oil. Essential oils were tested in vitro against three pathogen bacteria species by sensitivity test include disc diffusion assay （DD）, minimal inhibition concentration （MIC） and maximal bactericidal concentration （MBC）. Escherichia coli, Staphylococcus aureus and Salmonella typhimurium were used in this investigation. The results showed that the water and steam distillation and 3methanol： lethanol under tested by DD assay was found to be effective against all the pathogenic bacteria, in which the zone of inhibition exhibited E. coil S. aureus and S. typhimurium were 9.67 ± 0.35, 11.67 ± 0.66, 18.0± 1.15 and 9.00 ± 0.05, 8.3 ± 0.35, 19.0 ± 0.00 ram, respectively. While the hydro and ethanol distillations did action to against only E. coli which the inhibition zones were 8.67 ± 0.66 and 8.67 ± 0.35 mm, respectively. However, the commercial oil was more effective against tested pathogenic bacteria than all M. cordifolia essential oil. In case of MIC and MBC assays, the results showed that all essential oil distillation methods posed antibacterial potential in which the water and steam distillation showed the lowest MIC and MBC values against E. coli, S. aureus and S. typhimurium. It is suggested that among all M. cordifolia essential oil extractions, water and steam distillation was found to be highly bactericidal as it has shown in lowest MIC and MBC values and high in growth inhibition zone diameter.
