恙虫病东方体目视LAMP现场化检测方法的建立

目的建立基于“染料-蜡丸”的目视LAMP检测恙螨中恙虫病东方体的方法,为恙虫病的防治提供新工具。方法选择Ot GroEL基因作为检测靶序列,在线设计并合成LAMP引物。构建r Ot-GroEL重组质粒作为阳性对照。建立基于SYBR Green I的“染料-蜡丸”目视LAMP,并联合便携式LAMP仪使其利于现场应用。结果新设计的引物针对Ot GroEL基因的237 bp靶区域,所构建的重组质粒r Ot-GroEL测序结果正确。在便携式LAMP仪上运用“染料-蜡丸”目视LAMP法,其检测下限为1×10^2拷贝质粒。结论成功建立了一种可与便携式LAMP仪联合使用的检测恙螨体内恙虫病东方体的“染料-蜡丸”目视LAMP法。

Identification of Medical and Industrial Used Radioisotopes in Mining Sites of Nasarawa,Nasarawa State,Nigeria

This research intends to unveil the presence of radioisotopes in the soil of some mining sites in Nasarawa of Nasarawa state using thermos-scientific interceptor(IdentiFINDER).The work aimed at detecting the presence,types and trust level of radioisotopes.The result showed that,103Pd and 125I were found in 57%of the total points and the percentage abundance of the detector reached 50-65%indicating that,those radioisotopes are likely found in the area,109Cd was found in 15%of the total areas.The percentage abundance of the detector for 109Cd shows 50%indicating that,those radioisotopes are likely to be found in the area,241Am was found in 7%of the total areas.The percentage abundance of the detector for 241Am shows 81%indicating that,those radioisotopes are likely found in the area,235U was found in 7%of the total points.The percentage abundance of the detector for 235U reaches 57%indicating that,those radioisotopes are likely found in the area,75Se was found in 7%of the total points.The percentage abundance of the detector for 75Se was in abundance up to 57%indicating that,those radioisotopes are likely gotten in the area and 57Co was gotten in 7%of the total areas.The percentage abundance of the detector for 57Co was 54%indicating that,those radioisotopes are likely to be gotten in the area.Based on this high percentage abundance of the detector for these radioisotopes,they can be harnessed and applied appropriately in medicine and industry.