A new rapid, simple and reproducible UV spectrophotometric method was developed and validated for the estimation of Naproxen Sodium (NpSd) in bulk and pharmaceutical formulation. The quantification of NpSd was done at...A new rapid, simple and reproducible UV spectrophotometric method was developed and validated for the estimation of Naproxen Sodium (NpSd) in bulk and pharmaceutical formulation. The quantification of NpSd was done at 230 nm in methanol and in buffer of pH 6.8 and 9. Beer’s law was obeyed in the concentration range of 4 - 36 (r2 = 0.999) in methanol and 5 - 25 μgmL﹣1 in buffer of pH 6.8 and 9 (r2 = 0.988 and 0.997) respectively. The apparent molar absorptivity values were also calculated in all mediums. All parameters according to ICH guideline were tested and validated. The detection and quantitation limits were found to be 0.054, 0.083, 0.073 and 0.181, 0.251, 0.211 μgmL﹣1 respectively. These methods were applied directly to the analysis of the pharmaceutical tablet preparations (Anex? tablet 250 mg). The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation 3%), while being simple, cheap and less time consuming and hence can be suitably applied for the estimation of NpSd in dosage forms and dissolution studies.展开更多
Cotton(Gossypium hirsutum L.)is a key fiber crop of great commercial importance.Numerous phytopathogens decimate crop production by causing various diseases.During July-August 2018,leaf spot symptoms were recurrently ...Cotton(Gossypium hirsutum L.)is a key fiber crop of great commercial importance.Numerous phytopathogens decimate crop production by causing various diseases.During July-August 2018,leaf spot symptoms were recurrently observed on cotton leaves in Rahim Yar Khan,Pakistan and adjacent areas.Infected leaf samples were collected and plated on potato dextrose agar(PDA)media.Causal agent of cotton leaf spot was isolated,characterized and identified as Aspergillus tubingensis based on morphological and microscopic observations.Conclusive identification of pathogen was done on the comparative molecular analysis of CaM andβ-tubulin gene sequences.BLAST analysis of both sequenced genes showed 99%similarity with A.tubingensis.Koch’s postulates were followed to confirm the pathogenicity of the isolated fungus.Healthy plants were inoculated with fungus and similar disease symptoms were observed.Fungus was re-isolated and identified to be identical to the inoculated fungus.To our knowledge,this is the first report describing the involvement of A.tubingensis in causing leaf spot disease of cotton in Pakistan and around the world.展开更多
文摘A new rapid, simple and reproducible UV spectrophotometric method was developed and validated for the estimation of Naproxen Sodium (NpSd) in bulk and pharmaceutical formulation. The quantification of NpSd was done at 230 nm in methanol and in buffer of pH 6.8 and 9. Beer’s law was obeyed in the concentration range of 4 - 36 (r2 = 0.999) in methanol and 5 - 25 μgmL﹣1 in buffer of pH 6.8 and 9 (r2 = 0.988 and 0.997) respectively. The apparent molar absorptivity values were also calculated in all mediums. All parameters according to ICH guideline were tested and validated. The detection and quantitation limits were found to be 0.054, 0.083, 0.073 and 0.181, 0.251, 0.211 μgmL﹣1 respectively. These methods were applied directly to the analysis of the pharmaceutical tablet preparations (Anex? tablet 250 mg). The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation 3%), while being simple, cheap and less time consuming and hence can be suitably applied for the estimation of NpSd in dosage forms and dissolution studies.
基金This work was financially supported by university research fund(URF)for year 2017-18,Quaid-i-Azam University,Islamabad,Pakistan.
文摘Cotton(Gossypium hirsutum L.)is a key fiber crop of great commercial importance.Numerous phytopathogens decimate crop production by causing various diseases.During July-August 2018,leaf spot symptoms were recurrently observed on cotton leaves in Rahim Yar Khan,Pakistan and adjacent areas.Infected leaf samples were collected and plated on potato dextrose agar(PDA)media.Causal agent of cotton leaf spot was isolated,characterized and identified as Aspergillus tubingensis based on morphological and microscopic observations.Conclusive identification of pathogen was done on the comparative molecular analysis of CaM andβ-tubulin gene sequences.BLAST analysis of both sequenced genes showed 99%similarity with A.tubingensis.Koch’s postulates were followed to confirm the pathogenicity of the isolated fungus.Healthy plants were inoculated with fungus and similar disease symptoms were observed.Fungus was re-isolated and identified to be identical to the inoculated fungus.To our knowledge,this is the first report describing the involvement of A.tubingensis in causing leaf spot disease of cotton in Pakistan and around the world.
