Hemostatic genes in symptomatic and hidden forms of thrombotic complications in obstetrics.In given article we present the results of genetic testing for the factors of hemostasis in 30 patients during pregnancy and p...Hemostatic genes in symptomatic and hidden forms of thrombotic complications in obstetrics.In given article we present the results of genetic testing for the factors of hemostasis in 30 patients during pregnancy and postnatal period which constitute a single group due to common mechanisms of complications(thrombosis in placenta,central or peripheral veins)and clinically manifested by severe preeclampsia,intrauterine growth restriction,premature detachment of normally situated placenta,venous thrombosis during pregnancy and after delivery.We detectedС677Тpolymorphism of MTHFR gene in 33.3%,F5 G1691A in23.3%,F1G455A in 36.7%.The most frequent mutations were in genes of anticoagulant system:PAI-1 gene(93.3%in a general group;100%in the group of women with thrombosis),ACE gene(60%),ITGB(40%).In majority of observed cases we defined combinations of mutations of different genes.In all women of the study complications developed on a background of risk factors such as caesarian section,infection,hormonal therapy,bed regiment.We consider that both genetic polymorphisms and risk factors of thrombosis should be detected in early terms of pregnancy and presence of their combination requires a thromboprophylaxis during pregnancy and after delivery,especially after Cesarean section.展开更多
【Objective】To assess the impact of siRNA transfection on the tumor development under experimental pathology of thyroid gland.【Methods】Experiments were performed on rats weighing(180±20)g,which were divided in...【Objective】To assess the impact of siRNA transfection on the tumor development under experimental pathology of thyroid gland.【Methods】Experiments were performed on rats weighing(180±20)g,which were divided into five groups:IA and IB-animals with simulated hypo and hyperthyroid states and transplanted Guérin's carcinoma;ⅡA andⅡB-animals with simulated hypo and hyperthyroid states and transplanted Guérin's carcinoma in combination with siRNA transfection;Ⅲ-control group with transplanted Guérin's carcinoma in combination with siRNA transfection.Orthogonal dimensions of the tumor was measured.Histological and immunohistochemical researches of tumor samples were performed.【Results】It has been shown that the inhibitory effect of short interfering RNA realized in a greater extent in hypothyroid state,indicating an important role of thyroid hormones in the regulation of expression of genes that control the cell cycle.【Conclusion】siRNA transfection leads to inhibition of tumor growth in animals with both hypo and hyperthyroidism,but this process is more obvious in hypothyroidism status.展开更多
文摘Hemostatic genes in symptomatic and hidden forms of thrombotic complications in obstetrics.In given article we present the results of genetic testing for the factors of hemostasis in 30 patients during pregnancy and postnatal period which constitute a single group due to common mechanisms of complications(thrombosis in placenta,central or peripheral veins)and clinically manifested by severe preeclampsia,intrauterine growth restriction,premature detachment of normally situated placenta,venous thrombosis during pregnancy and after delivery.We detectedС677Тpolymorphism of MTHFR gene in 33.3%,F5 G1691A in23.3%,F1G455A in 36.7%.The most frequent mutations were in genes of anticoagulant system:PAI-1 gene(93.3%in a general group;100%in the group of women with thrombosis),ACE gene(60%),ITGB(40%).In majority of observed cases we defined combinations of mutations of different genes.In all women of the study complications developed on a background of risk factors such as caesarian section,infection,hormonal therapy,bed regiment.We consider that both genetic polymorphisms and risk factors of thrombosis should be detected in early terms of pregnancy and presence of their combination requires a thromboprophylaxis during pregnancy and after delivery,especially after Cesarean section.
文摘【Objective】To assess the impact of siRNA transfection on the tumor development under experimental pathology of thyroid gland.【Methods】Experiments were performed on rats weighing(180±20)g,which were divided into five groups:IA and IB-animals with simulated hypo and hyperthyroid states and transplanted Guérin's carcinoma;ⅡA andⅡB-animals with simulated hypo and hyperthyroid states and transplanted Guérin's carcinoma in combination with siRNA transfection;Ⅲ-control group with transplanted Guérin's carcinoma in combination with siRNA transfection.Orthogonal dimensions of the tumor was measured.Histological and immunohistochemical researches of tumor samples were performed.【Results】It has been shown that the inhibitory effect of short interfering RNA realized in a greater extent in hypothyroid state,indicating an important role of thyroid hormones in the regulation of expression of genes that control the cell cycle.【Conclusion】siRNA transfection leads to inhibition of tumor growth in animals with both hypo and hyperthyroidism,but this process is more obvious in hypothyroidism status.
