【Objective】To evaluate the roles of 80A→G polymorphism of RFC-1 gene encoding the reduced folate carrier protein and 677C→T polymorphism of 5,10-methylenetetrahydrofolate reductase gene(MTHFR)in neural tube defect...【Objective】To evaluate the roles of 80A→G polymorphism of RFC-1 gene encoding the reduced folate carrier protein and 677C→T polymorphism of 5,10-methylenetetrahydrofolate reductase gene(MTHFR)in neural tube defects(NTD)risk in Ukrainian population.【Methods】The folate status,homocysteine levels and genotypes were assessed in 42 mothers of fetuses with spina bifida,anencephaly and encephalocele with the age of 19-40 years.Serum folate and plasma homocysteine levels were estimated using chemiluminescence technology.DNA was isolated from peripheral leukocytes obtained from blood using standard procedures.The presence of the RFC-1 80A→G polymorphism was investigated using polymerase chain reaction(PCR).Real time PCR was used to detect the presence of the 677C→T mutation in the MTHFR gene.【Results】Genotype frequencies for RFC-1 80A→G in NTD group were:homozygous wild type in 9(21.4%),heterozygous type in16(38.1%)women;17(40.5%)women surveyed were found homozygous for the mutant allele genotype of RFC-1(GG).Homozygous for the indicated mutation was found in 57.1%of women with fetal anencephaly in the past history and 29.6%of women with spina bifida fetuses.Allelic frequency in this group of women for allele A was 40.5%(34),for allele G was 59.5%(50).Among women with 80A/A genotype of RFC-1 gene reduced levels of serum folic acid were noticed only in 6(35.5%),but hyperhomocysteinemia was found in 10(58.8%)women.【Conclusion】80G→A polymorphism of RFC-1 gene is a potential genetic factor in the formation of fetus NTD in women of South Ukraine.展开更多
The aim of the work was confined to the investigation of the epileptic activity dynamics under conditions of extremely high frequency electromagnetic waves(EHF) radiation(7.1 mm,0.1 mW/cm2) upon focal epileptic activi...The aim of the work was confined to the investigation of the epileptic activity dynamics under conditions of extremely high frequency electromagnetic waves(EHF) radiation(7.1 mm,0.1 mW/cm2) upon focal epileptic activity,which was penicillin-induced(10 000 IU/ml) in frontal zone of the brain cortex in myorelaxed and artificially ventilated rats under acute experimental condition.It was established that preliminarily performed hypogeomagnetic period(3.0 h) with the inductivity of geomagnetic field reduction not less than by 100 times caused the intensification of antiepileptic effects of EHF(15.0 min of exposition).It was pronounced in the form of decrease of foci intensity and significant reduction of life-span of foci-up to 115.3±13.4 minutes,which was both significant when compared with separate effects of hypogeomagnetic influence(187.3±12.5 min) and EHF(15.0 minutes of exposition) effect(164.2±12.5 minutes)(P <0.05).展开更多
文摘【Objective】To evaluate the roles of 80A→G polymorphism of RFC-1 gene encoding the reduced folate carrier protein and 677C→T polymorphism of 5,10-methylenetetrahydrofolate reductase gene(MTHFR)in neural tube defects(NTD)risk in Ukrainian population.【Methods】The folate status,homocysteine levels and genotypes were assessed in 42 mothers of fetuses with spina bifida,anencephaly and encephalocele with the age of 19-40 years.Serum folate and plasma homocysteine levels were estimated using chemiluminescence technology.DNA was isolated from peripheral leukocytes obtained from blood using standard procedures.The presence of the RFC-1 80A→G polymorphism was investigated using polymerase chain reaction(PCR).Real time PCR was used to detect the presence of the 677C→T mutation in the MTHFR gene.【Results】Genotype frequencies for RFC-1 80A→G in NTD group were:homozygous wild type in 9(21.4%),heterozygous type in16(38.1%)women;17(40.5%)women surveyed were found homozygous for the mutant allele genotype of RFC-1(GG).Homozygous for the indicated mutation was found in 57.1%of women with fetal anencephaly in the past history and 29.6%of women with spina bifida fetuses.Allelic frequency in this group of women for allele A was 40.5%(34),for allele G was 59.5%(50).Among women with 80A/A genotype of RFC-1 gene reduced levels of serum folic acid were noticed only in 6(35.5%),but hyperhomocysteinemia was found in 10(58.8%)women.【Conclusion】80G→A polymorphism of RFC-1 gene is a potential genetic factor in the formation of fetus NTD in women of South Ukraine.
文摘The aim of the work was confined to the investigation of the epileptic activity dynamics under conditions of extremely high frequency electromagnetic waves(EHF) radiation(7.1 mm,0.1 mW/cm2) upon focal epileptic activity,which was penicillin-induced(10 000 IU/ml) in frontal zone of the brain cortex in myorelaxed and artificially ventilated rats under acute experimental condition.It was established that preliminarily performed hypogeomagnetic period(3.0 h) with the inductivity of geomagnetic field reduction not less than by 100 times caused the intensification of antiepileptic effects of EHF(15.0 min of exposition).It was pronounced in the form of decrease of foci intensity and significant reduction of life-span of foci-up to 115.3±13.4 minutes,which was both significant when compared with separate effects of hypogeomagnetic influence(187.3±12.5 min) and EHF(15.0 minutes of exposition) effect(164.2±12.5 minutes)(P <0.05).
