The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-rel...The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-related macular degeneration,diabetic retinopathy,and glaucoma.Using visible light optical coherence tomography(vis-OCT),we demonstrated accurate and robust measurement of retinal oxygen metabolic rate(rMRO2)noninvasively in rat eyes.We continuously monitored the regulatory response of oxygen consumption to a progressive hypoxic challenge.We found that both oxygen delivery,and rMRO2 increased from the highly regulated retinal circulation(RC)under hypoxia,by 0.2860.08 μL min(^-1)(p,0.001),and 0.2060.04 μL min(^-1)(p,0.001)per 100 mmHg systemic pO2 reduction,respectively.The increased oxygen extraction compensated for the deficient oxygen supply from the poorly regulated choroidal circulation.Results from an oxygen diffusion model based on previous oxygen electrode measurements corroborated our in vivo observations.We believe that vis-OCT has the potential to reveal the fundamental role of oxygen metabolism in various retinal diseases.展开更多
Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultras...Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo.Here we demonstrate a novel optical imaging system,dual-band dual-scan inverse spectroscopic optical coherence tomography(D2-ISOCT),that,for the first time,can simultaneously obtain the following metrics in vivo using endogenous contrast:(1)capillary-level oxygen saturation and arteriolar-level blood flow rates,oxygen delivery rates,and oxygen metabolic rates;(2)spatial characteristics of tissue structures at length scales down to 30 nm;and(3)morphological images up to 2mm in depth.To illustrate the capabilities of D2-ISOCT,we monitored alterations to capillaries and the surrounding pericapillary tissue(tissue between the capillaries)in the healing response of a mouse ear wound model.The obtained microvascular and ultrastructural metrics corroborated well with each other,showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool.展开更多
Optical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location.We present a method termed spectral contrast optical coherence tomogra...Optical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location.We present a method termed spectral contrast optical coherence tomography angiography using visible light that relies on the spectral signatures of blood for angiography from a single scan using endogenous contrast.We demonstrate the molecular sensitivity of this method,which enables lymphatic vessel,blood,and tissue discrimination.展开更多
基金We would like to acknowledge the generous financial support from the NIH(Grant Nos 1R01EY019951 and 1R24EY022883)NSF(Grant Nos CBET-1055379 and CBET-1066776)+1 种基金Wenzhong Liu is supported by a HHMI graduate student fellowshipJi Yi is supported by a Seed Grant from the Illinois Society for Blindness Prevention and a post-doctoral fellowship award from the Juvenile Diabetes Research Foundation(JDRF).
文摘The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-related macular degeneration,diabetic retinopathy,and glaucoma.Using visible light optical coherence tomography(vis-OCT),we demonstrated accurate and robust measurement of retinal oxygen metabolic rate(rMRO2)noninvasively in rat eyes.We continuously monitored the regulatory response of oxygen consumption to a progressive hypoxic challenge.We found that both oxygen delivery,and rMRO2 increased from the highly regulated retinal circulation(RC)under hypoxia,by 0.2860.08 μL min(^-1)(p,0.001),and 0.2060.04 μL min(^-1)(p,0.001)per 100 mmHg systemic pO2 reduction,respectively.The increased oxygen extraction compensated for the deficient oxygen supply from the poorly regulated choroidal circulation.Results from an oxygen diffusion model based on previous oxygen electrode measurements corroborated our in vivo observations.We believe that vis-OCT has the potential to reveal the fundamental role of oxygen metabolism in various retinal diseases.
基金from the Evans Medical Foundation at Boston Medical Center,the National Institute of Health(R01CA173745,R01CA183101,and R01CA165309)the National Science Foundation(CBET-1240416).
文摘Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo.Here we demonstrate a novel optical imaging system,dual-band dual-scan inverse spectroscopic optical coherence tomography(D2-ISOCT),that,for the first time,can simultaneously obtain the following metrics in vivo using endogenous contrast:(1)capillary-level oxygen saturation and arteriolar-level blood flow rates,oxygen delivery rates,and oxygen metabolic rates;(2)spatial characteristics of tissue structures at length scales down to 30 nm;and(3)morphological images up to 2mm in depth.To illustrate the capabilities of D2-ISOCT,we monitored alterations to capillaries and the surrounding pericapillary tissue(tissue between the capillaries)in the healing response of a mouse ear wound model.The obtained microvascular and ultrastructural metrics corroborated well with each other,showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool.
基金provided by the National Science Foundation Graduate Research Fellowship and the National Institute of Health(R01CA200064,R01CA183101,R01CA173745,R01CA165309)provided by the INCITE programthe Argonne Leadership Computing Facility,which is a DOE Office of Science User Facility supported under Contract DE-AC02-06CH11357.
文摘Optical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location.We present a method termed spectral contrast optical coherence tomography angiography using visible light that relies on the spectral signatures of blood for angiography from a single scan using endogenous contrast.We demonstrate the molecular sensitivity of this method,which enables lymphatic vessel,blood,and tissue discrimination.
