Insect proteins have been proposed for human and animal food production.Safeguarding the health status of insects in mass rearing allows to obtain high-quality products and to avoid severe economic losses due to entom...Insect proteins have been proposed for human and animal food production.Safeguarding the health status of insects in mass rearing allows to obtain high-quality products and to avoid severe economic losses due to entomopathogens.Therefore,new strategies for preserving insect health must be implemented.Modulation of the insect im-mune system through the diet is one such strategy.We evaluated gene expression of two antimicrobial peptides(one defensin and one cecropin)in Hermetia illucens(L.)(Diptera:Stratiomyidae)reared on different diets.Analyses were performed on prepupae and 10-day-old larvae reared on cereal-and municipal organic waste-based diets and on only prepupae reared on a cereal-based diet supplemented with sunflower,corn,or soybean oil.The inclusion of sunflower oil at different points in the cereal-based diet was also evalu-ated.Moreover,diet-driven differences in the inhibitory activity of the hemolymph were tested against Escherichia coli DH5αand Micrococcus yunnanensis HI55 using diffusion assays in solid media.Results showed that a municipal organic waste-based diet produced a significant overexpression of antimicrobial peptides only in prepupae.Inclusion of veg-etable oils caused an upregulation of at least one peptide,except for the corn oil.Higher expression of both genes was observed when sunflower oil was added 5 days before pupa-tion.All hemolymph samples showed an inhibitory activity against bacteria colonies.Our results suggest that municipal organic waste-based diet and vegetable oil-added diet may successfully impact the immune system of H.illucens.Such alternatives may also exist forotherspeciesofeconomic interest.展开更多
文摘Insect proteins have been proposed for human and animal food production.Safeguarding the health status of insects in mass rearing allows to obtain high-quality products and to avoid severe economic losses due to entomopathogens.Therefore,new strategies for preserving insect health must be implemented.Modulation of the insect im-mune system through the diet is one such strategy.We evaluated gene expression of two antimicrobial peptides(one defensin and one cecropin)in Hermetia illucens(L.)(Diptera:Stratiomyidae)reared on different diets.Analyses were performed on prepupae and 10-day-old larvae reared on cereal-and municipal organic waste-based diets and on only prepupae reared on a cereal-based diet supplemented with sunflower,corn,or soybean oil.The inclusion of sunflower oil at different points in the cereal-based diet was also evalu-ated.Moreover,diet-driven differences in the inhibitory activity of the hemolymph were tested against Escherichia coli DH5αand Micrococcus yunnanensis HI55 using diffusion assays in solid media.Results showed that a municipal organic waste-based diet produced a significant overexpression of antimicrobial peptides only in prepupae.Inclusion of veg-etable oils caused an upregulation of at least one peptide,except for the corn oil.Higher expression of both genes was observed when sunflower oil was added 5 days before pupa-tion.All hemolymph samples showed an inhibitory activity against bacteria colonies.Our results suggest that municipal organic waste-based diet and vegetable oil-added diet may successfully impact the immune system of H.illucens.Such alternatives may also exist forotherspeciesofeconomic interest.
