Seedlessness represents a highly appreciated trait in table grapes.Based on an interesting case of seedless fruit production described in the crop species Annona squamosa,we focused on the Vitis vinifera INNER NO OUTE...Seedlessness represents a highly appreciated trait in table grapes.Based on an interesting case of seedless fruit production described in the crop species Annona squamosa,we focused on the Vitis vinifera INNER NO OUTER(INO)gene as a candidate.This gene encodes a transcription factor belonging to the YABBY family involved in the determination of abaxial identity in several organs.In Arabidopsis thaliana,this gene was shown to be essential for the formation and asymmetric growth of the ovule outer integument and its mutation leads to a phenotypic defect of ovules and failure in seed formation.In this study,we identified in silico the V.vinifera orthologue and investigated its phylogenetic relationship to INO genes from other species and its expression in different organs in seeded and seedless varieties.Applying cross-species complementation,we have tested its functionality in the Arabidopsis ino-1 mutant.We show that the V.vinifera INO successfully rescues the ovule outer integument growth and seeds set and also partially complements the outer integument asymmetric growth in the Arabidopsis mutant,differently from orthologues from other species.These data demonstrate that VviINO retains similar activity and protein targets in grapevine as in Arabidopsis.Potential implications for grapevine breeding are discussed.展开更多
The plant hormone jasmonic acid （JA） is essential for stress responses and the formation of reproductive organs, but its role in fruit development and ripening is unclear. Conjugation of JA to isoleucine is a crucia...The plant hormone jasmonic acid （JA） is essential for stress responses and the formation of reproductive organs, but its role in fruit development and ripening is unclear. Conjugation of JA to isoleucine is a crucial step in the JA signaling pathway since only JA-Ile is recognized by the jasmonate receptor. The conjugation reaction is catalyzed by JA-amido synthetases, belonging to the family of Gretchen Hagen3 （GH3） proteins. Here, in vitro studies of two grapevine （Vitis vinifera L. cv Shiraz） GH3 enzymes, VvGH3-7 and VvGH3-9, demonstrated JA-conjugating activ- ities with an overlapping range of amino acid substrates, including isoleucine. Expression studies of the correspond- ing genes in grape berries combined with JA and JA-lle measurements suggested a primary role for JA signaling in fruit set and cell division and did not support an involvement of JA in the ripening process. In response to methyl JA （MeJA） treatment, and in wounded and unwounded （distal）leaves, VvGH3-9 transcripts accumulated, indicating a participation in the JA response. In contrast, VvGH3-7 was unresponsive to MeJA and local wounding, demonstrating a differential transcriptional regulation of VvGH3-7 and VvGH3- 9. The transient induction of VvGH3-7 in unwounded, distal leaves was suggestive of the involvement of an unknown mobile wound signal.展开更多
文摘Seedlessness represents a highly appreciated trait in table grapes.Based on an interesting case of seedless fruit production described in the crop species Annona squamosa,we focused on the Vitis vinifera INNER NO OUTER(INO)gene as a candidate.This gene encodes a transcription factor belonging to the YABBY family involved in the determination of abaxial identity in several organs.In Arabidopsis thaliana,this gene was shown to be essential for the formation and asymmetric growth of the ovule outer integument and its mutation leads to a phenotypic defect of ovules and failure in seed formation.In this study,we identified in silico the V.vinifera orthologue and investigated its phylogenetic relationship to INO genes from other species and its expression in different organs in seeded and seedless varieties.Applying cross-species complementation,we have tested its functionality in the Arabidopsis ino-1 mutant.We show that the V.vinifera INO successfully rescues the ovule outer integument growth and seeds set and also partially complements the outer integument asymmetric growth in the Arabidopsis mutant,differently from orthologues from other species.These data demonstrate that VviINO retains similar activity and protein targets in grapevine as in Arabidopsis.Potential implications for grapevine breeding are discussed.
基金funded by Australia's grape growers and winemakers through their investment body the Grape and Wine ResearchDevelopment Corporation(grant no.CSP 09/05) with matching funding from the Australian Federal Government
文摘The plant hormone jasmonic acid （JA） is essential for stress responses and the formation of reproductive organs, but its role in fruit development and ripening is unclear. Conjugation of JA to isoleucine is a crucial step in the JA signaling pathway since only JA-Ile is recognized by the jasmonate receptor. The conjugation reaction is catalyzed by JA-amido synthetases, belonging to the family of Gretchen Hagen3 （GH3） proteins. Here, in vitro studies of two grapevine （Vitis vinifera L. cv Shiraz） GH3 enzymes, VvGH3-7 and VvGH3-9, demonstrated JA-conjugating activ- ities with an overlapping range of amino acid substrates, including isoleucine. Expression studies of the correspond- ing genes in grape berries combined with JA and JA-lle measurements suggested a primary role for JA signaling in fruit set and cell division and did not support an involvement of JA in the ripening process. In response to methyl JA （MeJA） treatment, and in wounded and unwounded （distal）leaves, VvGH3-9 transcripts accumulated, indicating a participation in the JA response. In contrast, VvGH3-7 was unresponsive to MeJA and local wounding, demonstrating a differential transcriptional regulation of VvGH3-7 and VvGH3- 9. The transient induction of VvGH3-7 in unwounded, distal leaves was suggestive of the involvement of an unknown mobile wound signal.
