Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is bas...Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00;as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 μM with a detection limit of 3.32 μM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA.展开更多
This study illustrates an innovative way to fabricate inkjet-printed tracks by sequential printing of Zn nanoparticle ink and curing ink for low temperature in situ chemical sintering.Employing chemical curing in plac...This study illustrates an innovative way to fabricate inkjet-printed tracks by sequential printing of Zn nanoparticle ink and curing ink for low temperature in situ chemical sintering.Employing chemical curing in place of standard sintering methods leads to the advantages of using flexible substrates that may not withstand the high thermal budgets of the standard methods.A general formulation engineering method is adopted to produce highly concentrated Zn ink which is cured by inkjet printing an over-layer of aqueous acetic acid which is the curing agent.The experimental results reveal that a narrow window of acid concentration of curing ink plays a crucial role in determining the electrical properties of the printed Zn nanoparticles.Highly conductive(~10^(5)S m^(−1))and mechanically flexible printed Zn features are achieved.In addition,from systematic material characterization,we obtain an understanding of the curing mechanism.Finally,a touch sensor circuit is demonstrated involving all-Zn printed conductive tracks.展开更多
The original version of this Article omitted from the author list the 3rd author Pawel Jerzy Wojcik from redoxme AB,Research&Development Department,Norrköping,Sweden(Affiliation#3)and 7th author Peter Dyrekle...The original version of this Article omitted from the author list the 3rd author Pawel Jerzy Wojcik from redoxme AB,Research&Development Department,Norrköping,Sweden(Affiliation#3)and 7th author Peter Dyreklev from RISE Research Institutes of Sweden,Norrköping,Sweden(Affiliation#1).展开更多
基金the Ministry of Higher Education Malaysia for the ERGS grant(600/RMI/st/ERGS/5/3/fst12/2011)Universiti Teknologi MARA for financial support via postgraduate teaching assistant scheme(UPTA)to Nur Azimah Mansor for conducting this research.
文摘Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00;as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 μM with a detection limit of 3.32 μM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA.
基金funded by European Union’s Horizon 2020 research and innovation program under grant agreement number 814485。
文摘This study illustrates an innovative way to fabricate inkjet-printed tracks by sequential printing of Zn nanoparticle ink and curing ink for low temperature in situ chemical sintering.Employing chemical curing in place of standard sintering methods leads to the advantages of using flexible substrates that may not withstand the high thermal budgets of the standard methods.A general formulation engineering method is adopted to produce highly concentrated Zn ink which is cured by inkjet printing an over-layer of aqueous acetic acid which is the curing agent.The experimental results reveal that a narrow window of acid concentration of curing ink plays a crucial role in determining the electrical properties of the printed Zn nanoparticles.Highly conductive(~10^(5)S m^(−1))and mechanically flexible printed Zn features are achieved.In addition,from systematic material characterization,we obtain an understanding of the curing mechanism.Finally,a touch sensor circuit is demonstrated involving all-Zn printed conductive tracks.
文摘The original version of this Article omitted from the author list the 3rd author Pawel Jerzy Wojcik from redoxme AB,Research&Development Department,Norrköping,Sweden(Affiliation#3)and 7th author Peter Dyreklev from RISE Research Institutes of Sweden,Norrköping,Sweden(Affiliation#1).
