A hundred Pseudomonas aeruginosa strains from several clinical specimens from five hospitals in Sao Luís-MA were evaluated for biofilm production, prevalence of the gene algD, adhesion to HEp-2 cells and antimicr...A hundred Pseudomonas aeruginosa strains from several clinical specimens from five hospitals in Sao Luís-MA were evaluated for biofilm production, prevalence of the gene algD, adhesion to HEp-2 cells and antimicrobial susceptibility. The most affected clinical specimens and hospital sectors were also evaluated. Most isolates were obtained from the tracheal aspirate (21.0%) and the most affected hospital sector was the ICU (43.0%). The antibiotics with the highest sensitivity rate were amikacin, piperacillin/tazobactam, fluoroquinolones, gentamicin and meropenem and the ones with the highest resistance rate were aztreonam, ceftazidime and cefepime. All samples were sensitive to polymyxin B. In relation to the expression of the gene for ESBL, 50.0% (17/34) of the multiresistant strains showed the enzyme TEM. Most strains showed high hydrophobicity and 96% of the isolates produced biofilm on a polystyrene microplate, 52% were capsule producers, 19% showed mannose-sensitive fimbriae and 39% expressed the gene algD. We observed adhesion to HEp-2 cells and to the coverslip. These factors may be reported in the pathogenesis of this bacterium, what represents a potential risk for colonization of medical devices which favor the establishment of chronic nosocomial infections.展开更多
Punica granatum L.possesses known antimicrobial and anti-inflammatory actions.Preparations of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have...Punica granatum L.possesses known antimicrobial and anti-inflammatory actions.Preparations of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have supported the use of P.granatum extracts for this purpose.However,little is known of the effects of P.granatum-derived fractions in severe infectious diseases such as sepsis.Here,we investigated the actions of the hexane fraction(HF),rich in lipophilic compounds,obtained from the leaves of this plant,in a murine model of polymicrobial sepsis.Oral pre-treatment with HF increased mortality in septic mice.The same animals presented with lower levels of interleukin-6,nitric oxide and hydrogen peroxide in their peritoneal lavage samples than vehicle mice.HF also increased polymorphonuclear cell accumulation in to the peritoneum of mice with sepsis.Macrophage-derived nitric oxide upon lipopolysaccharide stimuli was markedly reduced by incubation with HF.Diterpenes,triterpenes,phytosterols,vitamin E and ascorbyl palmitate were detected in the HF.These results indicate that P.granatum leaf lipophilic fractions may worsen sepsis outcome.This effect may be associated with its bioactive compounds which act synergistically or not,increasing sepsis mortality in vivo.展开更多
基金Fundacao de Amparoa Pesquisa e ao Desen-volvimento Cientifico e Tecnologico do Maranhao(FAP-EMA),for financial suports(ATB-04138/10,Edital FA-PEMA n 026/2010 Bancada,APP-Universal-00442/11).
文摘A hundred Pseudomonas aeruginosa strains from several clinical specimens from five hospitals in Sao Luís-MA were evaluated for biofilm production, prevalence of the gene algD, adhesion to HEp-2 cells and antimicrobial susceptibility. The most affected clinical specimens and hospital sectors were also evaluated. Most isolates were obtained from the tracheal aspirate (21.0%) and the most affected hospital sector was the ICU (43.0%). The antibiotics with the highest sensitivity rate were amikacin, piperacillin/tazobactam, fluoroquinolones, gentamicin and meropenem and the ones with the highest resistance rate were aztreonam, ceftazidime and cefepime. All samples were sensitive to polymyxin B. In relation to the expression of the gene for ESBL, 50.0% (17/34) of the multiresistant strains showed the enzyme TEM. Most strains showed high hydrophobicity and 96% of the isolates produced biofilm on a polystyrene microplate, 52% were capsule producers, 19% showed mannose-sensitive fimbriae and 39% expressed the gene algD. We observed adhesion to HEp-2 cells and to the coverslip. These factors may be reported in the pathogenesis of this bacterium, what represents a potential risk for colonization of medical devices which favor the establishment of chronic nosocomial infections.
文摘Punica granatum L.possesses known antimicrobial and anti-inflammatory actions.Preparations of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have supported the use of P.granatum extracts for this purpose.However,little is known of the effects of P.granatum-derived fractions in severe infectious diseases such as sepsis.Here,we investigated the actions of the hexane fraction(HF),rich in lipophilic compounds,obtained from the leaves of this plant,in a murine model of polymicrobial sepsis.Oral pre-treatment with HF increased mortality in septic mice.The same animals presented with lower levels of interleukin-6,nitric oxide and hydrogen peroxide in their peritoneal lavage samples than vehicle mice.HF also increased polymorphonuclear cell accumulation in to the peritoneum of mice with sepsis.Macrophage-derived nitric oxide upon lipopolysaccharide stimuli was markedly reduced by incubation with HF.Diterpenes,triterpenes,phytosterols,vitamin E and ascorbyl palmitate were detected in the HF.These results indicate that P.granatum leaf lipophilic fractions may worsen sepsis outcome.This effect may be associated with its bioactive compounds which act synergistically or not,increasing sepsis mortality in vivo.
