Objective:To enhance the pigment production byStreptomycessp.PM4 for evaluating its anticancer activity.Methods:Response surface methodology was employed to enhance the production of red pigment fromStreptomycessp.PM4...Objective:To enhance the pigment production byStreptomycessp.PM4 for evaluating its anticancer activity.Methods:Response surface methodology was employed to enhance the production of red pigment fromStreptomycessp.PM4.Optimized pigment was purified and evaluated for the anticancer activity against HT1080,Hep2,HeLa and MCF7 cell lines by MTT assay.Results:Based on the response surface methodology,it could be concluded that maltose (4.06 g),peptone (7.34 g),yeast extract(4.34 g)and tyrosine(2.89 g)were required for the maximum production of pigment(1.68 g/L)by theStreptomycessp.PM4.Optimization of the medium with the above tested features increased the pigment yield by 4.6 fold.Pigment showed the potential anticancer activity against HT1080,HEp-2,HeLa and MCF-7cell lines with the IC50value of 18.5,15.3,9.6 and 8.5 respectively.Conclusions:The study revealed that the maximum amount of pigment could be produced to treat cancer.展开更多
基金Financially supported by Ministry of Environment and Forests,India(Grant No.MoEn&F/22/27/2005-CSC)
文摘Objective:To enhance the pigment production byStreptomycessp.PM4 for evaluating its anticancer activity.Methods:Response surface methodology was employed to enhance the production of red pigment fromStreptomycessp.PM4.Optimized pigment was purified and evaluated for the anticancer activity against HT1080,Hep2,HeLa and MCF7 cell lines by MTT assay.Results:Based on the response surface methodology,it could be concluded that maltose (4.06 g),peptone (7.34 g),yeast extract(4.34 g)and tyrosine(2.89 g)were required for the maximum production of pigment(1.68 g/L)by theStreptomycessp.PM4.Optimization of the medium with the above tested features increased the pigment yield by 4.6 fold.Pigment showed the potential anticancer activity against HT1080,HEp-2,HeLa and MCF-7cell lines with the IC50value of 18.5,15.3,9.6 and 8.5 respectively.Conclusions:The study revealed that the maximum amount of pigment could be produced to treat cancer.
