Objective:To determine the profdes of anti-Entamoeba histolytica(E.histolytica) IgA.IgC. and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens.Methods:Serum samples from...Objective:To determine the profdes of anti-Entamoeba histolytica(E.histolytica) IgA.IgC. and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens.Methods:Serum samples from thirty diarrheic and thirty non-diarrheic individuals were subjected to IgA,IgG,and IgM profiling through enzyme-linked immunosorbent assay (EI.ISA),flow cytometry,and immunoblot.Results:ELBA titer results showed that both diarrheic and non-diarrheic individuals possess high levels of E.histolytica-specific IgG compared to IgA and IgM.How cytometry data showed that diarrheic serum samples had higher mean reaction percentages against E.histolytica cells compared to non-diarrheic samples.Immunoreactive E.histolytica proteins with molecular weights ranging belween 7 kDa and 292 kDa were recognized by diarrheic serum IgG,and 170 kDa and 250 kDa by non-diarrheic serum IgG. Conclusions:Our findings suggest that serum anti-E.histolytica IgG,compared with serum anti-E.histolytica IgA and IgM responses,was generally high in both diarrheic and nondiarrheic sera,indicating a past exposure to the organism both in symptomatic patients as well as in asymptomatic carriers,respectively.In addition,serum IgG from diarrheic and non-diarrheic patients were able to detect immunogenic E.histolytica proteins.展开更多
基金supported by a research grant from the Natural Sciences Research Institute of the University of the Philippines(B10-09-2-03) to W.L.R.
文摘Objective:To determine the profdes of anti-Entamoeba histolytica(E.histolytica) IgA.IgC. and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens.Methods:Serum samples from thirty diarrheic and thirty non-diarrheic individuals were subjected to IgA,IgG,and IgM profiling through enzyme-linked immunosorbent assay (EI.ISA),flow cytometry,and immunoblot.Results:ELBA titer results showed that both diarrheic and non-diarrheic individuals possess high levels of E.histolytica-specific IgG compared to IgA and IgM.How cytometry data showed that diarrheic serum samples had higher mean reaction percentages against E.histolytica cells compared to non-diarrheic samples.Immunoreactive E.histolytica proteins with molecular weights ranging belween 7 kDa and 292 kDa were recognized by diarrheic serum IgG,and 170 kDa and 250 kDa by non-diarrheic serum IgG. Conclusions:Our findings suggest that serum anti-E.histolytica IgG,compared with serum anti-E.histolytica IgA and IgM responses,was generally high in both diarrheic and nondiarrheic sera,indicating a past exposure to the organism both in symptomatic patients as well as in asymptomatic carriers,respectively.In addition,serum IgG from diarrheic and non-diarrheic patients were able to detect immunogenic E.histolytica proteins.
