The relationship between a polymorphism at position - 670 in the Fas gene (TNFRSF6) and preterm premature rupture of membranes (PPROM) in multifetal pregnancies was examined. Buccal swabs from 119 mother- infant sets ...The relationship between a polymorphism at position - 670 in the Fas gene (TNFRSF6) and preterm premature rupture of membranes (PPROM) in multifetal pregnancies was examined. Buccal swabs from 119 mother- infant sets were analyzed for an adenine (A) to guanine (G) substitution at position - 670 in the TNFRSF6 promoter. Pregnancy outcome data were subsequently obtained. Analysis was by Fisher exact test. Maternal allele G homozygosity (TNFRSF6* G) was observed in 42.4% of 33 PPROM pregnancies as opposed to 19.5% of 77 with no spontaneous preterm birth (P =. 01). Similarly, TNFRSF6* G homozygosity was present in 37.5% of 32 first- born neonates from PPROM pregnancies as opposed to 18.7% of 75 uncomplicated pregnancies (P =. 04). PPROM occurred in 8 of 14 (57.1% ) pregnancies in which mother and all neonates were TNFRSF6* G homozygotes as opposed to 25 of 105 (23.8% )- cases in which uniform TNFRSF6* G homozygosity was not observed (P =. 02). A genetic variant in the Fas gene is associated with an increased rate of PPROM in multifetal pregnancies.展开更多
The association between the detection of Mycoplasma hominis or Ureaplasma urealyticum in midtrimester amniotic fluid and amniotic fluid cytokine concentrations and subsequent pregnancy outcome were examined. Amniocent...The association between the detection of Mycoplasma hominis or Ureaplasma urealyticum in midtrimester amniotic fluid and amniotic fluid cytokine concentrations and subsequent pregnancy outcome were examined. Amniocentesis was performed between 15 and 19 weeks of gestation in 179 asymptomatic women. Aliquots were assayed for M hominis and U urealyticum by polymerase chain reaction coupled to enzyme linked immunosorbent assay. Intra amniotic levels of interleukin- 1β , interleukin- 1 receptor antagonist, interleukin- 4, interleukin- 6, and tumor necrosis factor- α were determined by enzyme linked immunosorbent assay. Pregnancy outcomes were obtained after the completion of all testing. U urealyticum was detected in 22 of 172 amniotic fluids (12.8% ); M hominis was present in 11 of 179 amniotic fluids (6.1% ). There was no relationship between U urealyticum detection and the concentration of any cytokine. Detection of M hominis was associated with elevated intra amniotic concentrations of interleukin- 4 (P =. 01). Preterm premature rupture of membranes that was followed by preterm birth occurred in 5 women (2.8% ); 5 women (2.8% )- had a spontaneous preterm birth with intact membranes. All 5 of the women with preterm premature rupture of membranes (100% ) tested positive for either U urealyticum or M hominis, as opposed to none of the women with spontaneous preterm birth and to 27 of 161 women (16.8% ) with a term birth (P =. 0002). The detection of M hominis or U urealyticum in midtrimester amniotic fluid by polymerase chain reaction enzyme linked immunosorbent assay may be a risk factor for subsequent preterm premature rupture of membranes.展开更多
文摘The relationship between a polymorphism at position - 670 in the Fas gene (TNFRSF6) and preterm premature rupture of membranes (PPROM) in multifetal pregnancies was examined. Buccal swabs from 119 mother- infant sets were analyzed for an adenine (A) to guanine (G) substitution at position - 670 in the TNFRSF6 promoter. Pregnancy outcome data were subsequently obtained. Analysis was by Fisher exact test. Maternal allele G homozygosity (TNFRSF6* G) was observed in 42.4% of 33 PPROM pregnancies as opposed to 19.5% of 77 with no spontaneous preterm birth (P =. 01). Similarly, TNFRSF6* G homozygosity was present in 37.5% of 32 first- born neonates from PPROM pregnancies as opposed to 18.7% of 75 uncomplicated pregnancies (P =. 04). PPROM occurred in 8 of 14 (57.1% ) pregnancies in which mother and all neonates were TNFRSF6* G homozygotes as opposed to 25 of 105 (23.8% )- cases in which uniform TNFRSF6* G homozygosity was not observed (P =. 02). A genetic variant in the Fas gene is associated with an increased rate of PPROM in multifetal pregnancies.
文摘The association between the detection of Mycoplasma hominis or Ureaplasma urealyticum in midtrimester amniotic fluid and amniotic fluid cytokine concentrations and subsequent pregnancy outcome were examined. Amniocentesis was performed between 15 and 19 weeks of gestation in 179 asymptomatic women. Aliquots were assayed for M hominis and U urealyticum by polymerase chain reaction coupled to enzyme linked immunosorbent assay. Intra amniotic levels of interleukin- 1β , interleukin- 1 receptor antagonist, interleukin- 4, interleukin- 6, and tumor necrosis factor- α were determined by enzyme linked immunosorbent assay. Pregnancy outcomes were obtained after the completion of all testing. U urealyticum was detected in 22 of 172 amniotic fluids (12.8% ); M hominis was present in 11 of 179 amniotic fluids (6.1% ). There was no relationship between U urealyticum detection and the concentration of any cytokine. Detection of M hominis was associated with elevated intra amniotic concentrations of interleukin- 4 (P =. 01). Preterm premature rupture of membranes that was followed by preterm birth occurred in 5 women (2.8% ); 5 women (2.8% )- had a spontaneous preterm birth with intact membranes. All 5 of the women with preterm premature rupture of membranes (100% ) tested positive for either U urealyticum or M hominis, as opposed to none of the women with spontaneous preterm birth and to 27 of 161 women (16.8% ) with a term birth (P =. 0002). The detection of M hominis or U urealyticum in midtrimester amniotic fluid by polymerase chain reaction enzyme linked immunosorbent assay may be a risk factor for subsequent preterm premature rupture of membranes.
