Objective:To examine the downregulalion of proinflammatory cytokines in a time dependant manner on carbon tetrachloride induced toxicity in experimental animals.Methods:CCl<sub>4</sub>（150μL/100 g） was ...Objective:To examine the downregulalion of proinflammatory cytokines in a time dependant manner on carbon tetrachloride induced toxicity in experimental animals.Methods:CCl<sub>4</sub>（150μL/100 g） was dissolved in com oil（1:1 v/v%） and administered orally,GroupⅠwas treated as normal control and received corn oil on 8th day.GroupⅡwas toxic control and was given a single dose of CCl<sub>4</sub> on 8th days.GroupⅢwas treated with Lygodium flexuosum（L.flexuosum） n-hexane extract（200 mg/kg） for 8 days and on 8th day a single dose of CCl<sub>4</sub> was received.GroupⅣ（negative contnil） received L.flexuosiim n-hexane extract（200 mg/kg） alone for 8 days.Results:Treatment with n-hexane extract prior to the administration of CCl<sub>4</sub> significantly prevented an increase in serum AST,ALT,LDH activity and lipid peroxidation and prevented the depletion of glutathione （GSH）.Rats treated with L.flexuosum had reduced mRNA levels of TGF-β1,TNF-αand IL-1βgenes in liver of CCl<sub>4</sub> intoxicated rats when compared to CCl<sub>4</sub> control as evidenced by RT-PCR. Conclusions:The data suggest that L.flexuosum,a widely available fem,significantly reduces CCl<sub>4</sub> induced acute hepatotoxicily by down-regulating the expression of pro-inflammatory cytokines in rats.展开更多
基金supported by grants from kerala Forest Department, kerala India Financial assistance from Council of Scientific and Industrial Research(CSIR),New Delhi as Senior Research Fellowship to PJ Wills
文摘Objective:To examine the downregulalion of proinflammatory cytokines in a time dependant manner on carbon tetrachloride induced toxicity in experimental animals.Methods:CCl<sub>4</sub>（150μL/100 g） was dissolved in com oil（1:1 v/v%） and administered orally,GroupⅠwas treated as normal control and received corn oil on 8th day.GroupⅡwas toxic control and was given a single dose of CCl<sub>4</sub> on 8th days.GroupⅢwas treated with Lygodium flexuosum（L.flexuosum） n-hexane extract（200 mg/kg） for 8 days and on 8th day a single dose of CCl<sub>4</sub> was received.GroupⅣ（negative contnil） received L.flexuosiim n-hexane extract（200 mg/kg） alone for 8 days.Results:Treatment with n-hexane extract prior to the administration of CCl<sub>4</sub> significantly prevented an increase in serum AST,ALT,LDH activity and lipid peroxidation and prevented the depletion of glutathione （GSH）.Rats treated with L.flexuosum had reduced mRNA levels of TGF-β1,TNF-αand IL-1βgenes in liver of CCl<sub>4</sub> intoxicated rats when compared to CCl<sub>4</sub> control as evidenced by RT-PCR. Conclusions:The data suggest that L.flexuosum,a widely available fem,significantly reduces CCl<sub>4</sub> induced acute hepatotoxicily by down-regulating the expression of pro-inflammatory cytokines in rats.
