Starch is an attractive polymer for wound healing applications because of its wide availability,low cost,biocompatibility,biodegradability and wound-healing property.Here,we have fabricated starch-based nanofibrous sc...Starch is an attractive polymer for wound healing applications because of its wide availability,low cost,biocompatibility,biodegradability and wound-healing property.Here,we have fabricated starch-based nanofibrous scaffolds by electrospinning for wound healing applications.The diameter of the optimized nanofibers was determined by field emission scanning electron microscopy(FE-SEM)and was found to be in the range of 110e300 nm.The mechanical strength(0.5e0.8 MPa)of the nanofibrous scaffolds was attuned using polyvinyl alcohol(plasticizer)and glutaraldehyde(crosslinking agent),to impart them with sufficient durability for skin tissue engineering.Absence of negative interactions between the polymers was confirmed by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy(ATR-FTIR),differential scanning microscopy(DSC)and thermal gravimetric analysis(TGA).Cellular assays with L929 mouse fibroblast cells indicated the ability of the scaffolds to promote cellular proliferation,without exhibiting any toxic effect to the cells.Thus,the nanofibrous scaffolds demonstrated potential for wound healing applications.展开更多
基金Ms.VijayaWaghmare is thankful to Technical Education Quality Improvement Program(TEQIP),Government of India for research fellowship.The authors wish to thank Ramanujan Fellowship Grant(SR/S2/RJN-139/2011)Department of Science and Technology,Government of India+2 种基金Ramalingaswami Fellowship Grant(BT/RLF/Re-entry/51/2011)Department of Biotechnology,Government of India,and Rajiv Gandhi Science and Technology Commission(RGSTC/File-2013/DPP-108/CR-19)Government of Maharashtra,for funding.
文摘Starch is an attractive polymer for wound healing applications because of its wide availability,low cost,biocompatibility,biodegradability and wound-healing property.Here,we have fabricated starch-based nanofibrous scaffolds by electrospinning for wound healing applications.The diameter of the optimized nanofibers was determined by field emission scanning electron microscopy(FE-SEM)and was found to be in the range of 110e300 nm.The mechanical strength(0.5e0.8 MPa)of the nanofibrous scaffolds was attuned using polyvinyl alcohol(plasticizer)and glutaraldehyde(crosslinking agent),to impart them with sufficient durability for skin tissue engineering.Absence of negative interactions between the polymers was confirmed by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy(ATR-FTIR),differential scanning microscopy(DSC)and thermal gravimetric analysis(TGA).Cellular assays with L929 mouse fibroblast cells indicated the ability of the scaffolds to promote cellular proliferation,without exhibiting any toxic effect to the cells.Thus,the nanofibrous scaffolds demonstrated potential for wound healing applications.
