One of the main goals of vaccine research is the development of adjuvants that can enhance immune responses and are both safe and biocompatible.We explored the application of the natural polymer hyaluronan(HA)as a pro...One of the main goals of vaccine research is the development of adjuvants that can enhance immune responses and are both safe and biocompatible.We explored the application of the natural polymer hyaluronan(HA)as a promising immunological adjuvant for protein-based vaccines.Chemical conjugation of HA to antigens strongly increased their immunogenicity,reduced booster requirements,and allowed antigen dose sparing.HA-based bioconjugates stimulated robust and long-lasting humoral responses without the addition of other immunostimulatory compounds and proved highly efficient when compared to other adjuvants.Due to its intrinsic biocompatibility,HA allowed the exploitation of different injection routes and did not induce inflammation at the inoculation site.This polymer promoted rapid translocation of the antigen to draining lymph nodes,thus facilitating encounters with antigen-presenting cells.Overall,HA can be regarded as an effective and biocompatible adjuvant to be exploited for the design of a wide variety of vaccines.展开更多
基金supported by Fondazione AIRC under IG 2018-ID.21354 project-P.I.Rosato Antonio,5 per Mille 2019-ID.22759 program-G.L.Rosato Antonio,BIGID219MON2 from 5 per Mille 2018Veneto Institute of Oncology IOV-IRCCS to I.M.M.,Bando Ricerca Covid 2019 from Fondazione Cassa di Risparmio di Padova e Rovigo.P.D.B.wishes to thank the Italian Ministry of Health,which partially contributed to the achievements of the study through RC IZSVE 08/06.
文摘One of the main goals of vaccine research is the development of adjuvants that can enhance immune responses and are both safe and biocompatible.We explored the application of the natural polymer hyaluronan(HA)as a promising immunological adjuvant for protein-based vaccines.Chemical conjugation of HA to antigens strongly increased their immunogenicity,reduced booster requirements,and allowed antigen dose sparing.HA-based bioconjugates stimulated robust and long-lasting humoral responses without the addition of other immunostimulatory compounds and proved highly efficient when compared to other adjuvants.Due to its intrinsic biocompatibility,HA allowed the exploitation of different injection routes and did not induce inflammation at the inoculation site.This polymer promoted rapid translocation of the antigen to draining lymph nodes,thus facilitating encounters with antigen-presenting cells.Overall,HA can be regarded as an effective and biocompatible adjuvant to be exploited for the design of a wide variety of vaccines.
