Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medi...Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts.展开更多
本系列病例的目的,是将一种新型可吸收胶原基质(collagen matrix,CMX)应用于美学区种植同期行引导骨再生(guided bone regeneration.GBR)术中.评估其在8周愈合期内增强组织厚度的安全性和可行性。使用超声波装置测定种植位点和邻牙的软...本系列病例的目的,是将一种新型可吸收胶原基质(collagen matrix,CMX)应用于美学区种植同期行引导骨再生(guided bone regeneration.GBR)术中.评估其在8周愈合期内增强组织厚度的安全性和可行性。使用超声波装置测定种植位点和邻牙的软组织厚度。通过连续数字表面模型叠加牙周参数评估整体组织轮廓变化。牙周参数和患者相关结果显示无显著变化。GBR联合新型CMX使得植入部位的软组织厚度在8周后显著增加156mm.且从4周到8周软组织没有显著减少。整体组织轮廓在距粘膜边缘5mm处增加最为显著.与种植体肩部区域的组织增加相对应。8周后邻牙未见受到影响。展开更多
基金supported by a grant from the ITI International Team for Implantology Foundation 1235_2017 to M.B.A
文摘Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts.
文摘本系列病例的目的,是将一种新型可吸收胶原基质(collagen matrix,CMX)应用于美学区种植同期行引导骨再生(guided bone regeneration.GBR)术中.评估其在8周愈合期内增强组织厚度的安全性和可行性。使用超声波装置测定种植位点和邻牙的软组织厚度。通过连续数字表面模型叠加牙周参数评估整体组织轮廓变化。牙周参数和患者相关结果显示无显著变化。GBR联合新型CMX使得植入部位的软组织厚度在8周后显著增加156mm.且从4周到8周软组织没有显著减少。整体组织轮廓在距粘膜边缘5mm处增加最为显著.与种植体肩部区域的组织增加相对应。8周后邻牙未见受到影响。
