One hundred ninety-two crossbred steers (229 kg) were used to evaluate the influence of metabolizable amino acid intake on growth performance and health during the initial 42-d receiving period. Treatments consisted o...One hundred ninety-two crossbred steers (229 kg) were used to evaluate the influence of metabolizable amino acid intake on growth performance and health during the initial 42-d receiving period. Treatments consisted of four levels of metabolizable lysine (23, 24, 25 and 26 g/kg diet DM). Morbidity averaged 36%, and was not affected (P > 0.20) by treatments. No steers died during the study. Increasing the metabolizable lysine supply increased DMI, ADG, gain efficiency, and dietary NE (linear effect, P < 0.01). Metabolizable amino acid supply of the basal diet was determined using 6 steers (214 kg) with cannulas in the rumen and proximal duodenum. Metabolizable amino acid supply of the diet was in close agreement with expected (R2 = 0.99;P < 0.0001) based on NRC (2000) [12] Level 1 model. The metabolizable amino acid supplies for treatments in Trial 1 were estimated by adding tabular metabolizable amino acid values for the respective supplemental proteins (cottonseed meal and fish meal) to the observed metabolizable amino acid supply of the basal diet. Treatment effects on metabolizable lysine supply explained 99% of the variation (P < 0.01) in ADG, and 91% of the variation in observed versus expected dietary NE. The biological value for the intestinal chyme was determined based on chemical score technique, using bovine tissue as the reference protein. Accordingly, methionine and lysine were closely co-limiting amino acids having ratios of 77% and 79%, respectively. We conclude that current NRC standards reliably predict both requirements and supplies of metabolizable amino acids for feedlot calves. Diet formulations that do not meet the metabolizable amino acid requirements may depress both ADG and the partial efficiency of utilization of metabolizable energy for maintenance and gain.展开更多
文摘One hundred ninety-two crossbred steers (229 kg) were used to evaluate the influence of metabolizable amino acid intake on growth performance and health during the initial 42-d receiving period. Treatments consisted of four levels of metabolizable lysine (23, 24, 25 and 26 g/kg diet DM). Morbidity averaged 36%, and was not affected (P > 0.20) by treatments. No steers died during the study. Increasing the metabolizable lysine supply increased DMI, ADG, gain efficiency, and dietary NE (linear effect, P < 0.01). Metabolizable amino acid supply of the basal diet was determined using 6 steers (214 kg) with cannulas in the rumen and proximal duodenum. Metabolizable amino acid supply of the diet was in close agreement with expected (R2 = 0.99;P < 0.0001) based on NRC (2000) [12] Level 1 model. The metabolizable amino acid supplies for treatments in Trial 1 were estimated by adding tabular metabolizable amino acid values for the respective supplemental proteins (cottonseed meal and fish meal) to the observed metabolizable amino acid supply of the basal diet. Treatment effects on metabolizable lysine supply explained 99% of the variation (P < 0.01) in ADG, and 91% of the variation in observed versus expected dietary NE. The biological value for the intestinal chyme was determined based on chemical score technique, using bovine tissue as the reference protein. Accordingly, methionine and lysine were closely co-limiting amino acids having ratios of 77% and 79%, respectively. We conclude that current NRC standards reliably predict both requirements and supplies of metabolizable amino acids for feedlot calves. Diet formulations that do not meet the metabolizable amino acid requirements may depress both ADG and the partial efficiency of utilization of metabolizable energy for maintenance and gain.
