设计一种高效环保的健身自行车的发电、蓄电和用电系统。利用脚蹬踏板带动发电机转子旋转发电,再利用齿轮变速装置改变转速控制输出电能的大小对蓄电池进行充电,经过电力电子装置改善电能后分两路输出,一路直流5 V USB接口供手机充电,...设计一种高效环保的健身自行车的发电、蓄电和用电系统。利用脚蹬踏板带动发电机转子旋转发电,再利用齿轮变速装置改变转速控制输出电能的大小对蓄电池进行充电,经过电力电子装置改善电能后分两路输出,一路直流5 V USB接口供手机充电,一路交流220 V工频供小功率负载使用。此外,需要时装置还能将储存的电能供给给电动机,提供动能。展开更多
Phenylalanine ammonia-lyase(PAL),which catalyzes the conversion from L-phenylalanine to trans-cinnamic acid,is a well-known key enzyme and a connecting step between primary and secondary metabolisms in the phenylpropa...Phenylalanine ammonia-lyase(PAL),which catalyzes the conversion from L-phenylalanine to trans-cinnamic acid,is a well-known key enzyme and a connecting step between primary and secondary metabolisms in the phenylpropanoid biosynthetic pathway of plants and microbes.Schisandra chinensis,a woody vine plant belonging to the family of Magnoliaceae,is a rich source of dibenzocyclooctadiene lignans exhibiting potent activity.However,the functional role of PAL in the biosynthesis of lignan is relatively limited,compared with those in lignin and flavonoids biosynthesis.Therefore,it is essential to clone and characterize the PAL genes from this valuable medicinal plant.In this study,molecular cloning and characterization of three PAL genes(ScPAL1−3)from S.chinensis was carried out.ScPALs were cloned using RACE PCR.The sequence analysis of the three ScPALs was carried out to give basic characteristics followed by docking analysis.In order to determine their catalytic activity,recombinant protein was obtained by heterologous expression in pCold-TF vector in Escherichia coli(BL21-DE3),followed by Ni-affinity purification.The catalytic product of the purified recombinant proteins was verified using RP-HPLC through comparing with standard compounds.The optimal temperature,pH value and effects of different metal ions were determined.Vmax,Kcat and Km values were determined under the optimal conditions.The expression of three ScPALs in different tissues was also determined.Our work provided essential information for the function of ScPALs.展开更多
文摘设计一种高效环保的健身自行车的发电、蓄电和用电系统。利用脚蹬踏板带动发电机转子旋转发电,再利用齿轮变速装置改变转速控制输出电能的大小对蓄电池进行充电,经过电力电子装置改善电能后分两路输出,一路直流5 V USB接口供手机充电,一路交流220 V工频供小功率负载使用。此外,需要时装置还能将储存的电能供给给电动机,提供动能。
基金supported by the National Key R&D Program of China(No.2017YFC1701200).
文摘Phenylalanine ammonia-lyase(PAL),which catalyzes the conversion from L-phenylalanine to trans-cinnamic acid,is a well-known key enzyme and a connecting step between primary and secondary metabolisms in the phenylpropanoid biosynthetic pathway of plants and microbes.Schisandra chinensis,a woody vine plant belonging to the family of Magnoliaceae,is a rich source of dibenzocyclooctadiene lignans exhibiting potent activity.However,the functional role of PAL in the biosynthesis of lignan is relatively limited,compared with those in lignin and flavonoids biosynthesis.Therefore,it is essential to clone and characterize the PAL genes from this valuable medicinal plant.In this study,molecular cloning and characterization of three PAL genes(ScPAL1−3)from S.chinensis was carried out.ScPALs were cloned using RACE PCR.The sequence analysis of the three ScPALs was carried out to give basic characteristics followed by docking analysis.In order to determine their catalytic activity,recombinant protein was obtained by heterologous expression in pCold-TF vector in Escherichia coli(BL21-DE3),followed by Ni-affinity purification.The catalytic product of the purified recombinant proteins was verified using RP-HPLC through comparing with standard compounds.The optimal temperature,pH value and effects of different metal ions were determined.Vmax,Kcat and Km values were determined under the optimal conditions.The expression of three ScPALs in different tissues was also determined.Our work provided essential information for the function of ScPALs.
