探讨了乙酸铵交换-容量法测定土壤阳离子交换量过程中影响测定结果的关键分析条件。试验结果表明,最佳分析条件是:土壤样品粒径为20目,土壤样品与乙酸铵溶液的固液比为2 g∶50 m L,振荡频率为240 r/min、振荡时间为4 min,铵离子洗脱剂...探讨了乙酸铵交换-容量法测定土壤阳离子交换量过程中影响测定结果的关键分析条件。试验结果表明,最佳分析条件是:土壤样品粒径为20目,土壤样品与乙酸铵溶液的固液比为2 g∶50 m L,振荡频率为240 r/min、振荡时间为4 min,铵离子洗脱剂乙醇使用量为150 m L(均分3次),水蒸气蒸馏的馏出液体积为120 m L,用盐酸标准溶液容量法测定土壤阳离子交换量。结果表明,方法检出限为1.0 cmol/kg,测定下限为4.0 cmol/kg,代表性样品(p H值=5.69~8.13)测定结果的相对标准偏差为2.4%~4.6%(n=6),土壤标准样品测定结果与标准值相符,说明方法具有良好的适用性,能够满足国家土壤环境质量监测及农用地详查分析工作质量要求。展开更多
Wheat （Triticum aestivum L.） line Lankao 90（6） carries a recessive powdery mildew resistance gene temporarily named PmLK906 on chromosome 2AL. Near PmLK906 there is another known powdery mildew resistance gene loc...Wheat （Triticum aestivum L.） line Lankao 90（6） carries a recessive powdery mildew resistance gene temporarily named PmLK906 on chromosome 2AL. Near PmLK906 there is another known powdery mildew resistance gene locus Pm4. To track the two powdery mildew resistance genes in wheat breeding program by marker assisted selection （MAS）, a linked molecular marker was developed in this study. Wheat gene chip hybridization combined with bulked segregant analysis （BSA） was used to develop an sequence-tagged sites （STS） marker for PmLK906 and Pm4. A new 2 125 bp full-length cDNA clone （GenBank accession no. EU082094） similar to csAtPR5 ofAegilops tauschii was isolated from Lankao 90（6） 21-12, and temporarily named TaAetPR5. Specific products could be amplified from cultivars or lines possessing Pm4a, Pm4b and PmLK906 with primers p9-7pl and p9-7p2 derived from TaAetPR5. TaAetPR5 was linked to PmLK906 at a genetic distance of 7.62 cM, and cosegregated with Pm4a. The p9-7p1 and p9-7p2 could be used as an STS marker for these resistance genes in wheat breeding. Because this marker was cosegregated with Pm4a, it can be used in map-based cloning of the alleles at Pm4 locus also.展开更多
文摘探讨了乙酸铵交换-容量法测定土壤阳离子交换量过程中影响测定结果的关键分析条件。试验结果表明,最佳分析条件是:土壤样品粒径为20目,土壤样品与乙酸铵溶液的固液比为2 g∶50 m L,振荡频率为240 r/min、振荡时间为4 min,铵离子洗脱剂乙醇使用量为150 m L(均分3次),水蒸气蒸馏的馏出液体积为120 m L,用盐酸标准溶液容量法测定土壤阳离子交换量。结果表明,方法检出限为1.0 cmol/kg,测定下限为4.0 cmol/kg,代表性样品(p H值=5.69~8.13)测定结果的相对标准偏差为2.4%~4.6%(n=6),土壤标准样品测定结果与标准值相符,说明方法具有良好的适用性,能够满足国家土壤环境质量监测及农用地详查分析工作质量要求。
基金supported by the Innovation Fund for Outstanding Scholars of Henan Province, China(0621001700)
文摘Wheat （Triticum aestivum L.） line Lankao 90（6） carries a recessive powdery mildew resistance gene temporarily named PmLK906 on chromosome 2AL. Near PmLK906 there is another known powdery mildew resistance gene locus Pm4. To track the two powdery mildew resistance genes in wheat breeding program by marker assisted selection （MAS）, a linked molecular marker was developed in this study. Wheat gene chip hybridization combined with bulked segregant analysis （BSA） was used to develop an sequence-tagged sites （STS） marker for PmLK906 and Pm4. A new 2 125 bp full-length cDNA clone （GenBank accession no. EU082094） similar to csAtPR5 ofAegilops tauschii was isolated from Lankao 90（6） 21-12, and temporarily named TaAetPR5. Specific products could be amplified from cultivars or lines possessing Pm4a, Pm4b and PmLK906 with primers p9-7pl and p9-7p2 derived from TaAetPR5. TaAetPR5 was linked to PmLK906 at a genetic distance of 7.62 cM, and cosegregated with Pm4a. The p9-7p1 and p9-7p2 could be used as an STS marker for these resistance genes in wheat breeding. Because this marker was cosegregated with Pm4a, it can be used in map-based cloning of the alleles at Pm4 locus also.
