Research on hsa-miR-155-3p and hsa-miR-155-5p as biomarkers for systemic sclerosis and their role in regulating biological behavior

Objective: This study was to investigate the role of hsa-miR-155-3p and hsa-miR-155-5p as biomarkers and regulators of biological behavior in Systemic Sclerosis. Methods: A total of 10 SSc patients and 10 healthy controls were selected for the study. The expression levels of hsa-miR-155-3p and hsa-miR-155-5p in peripheral blood mononuclear cells of SSc patients and healthy controls were measured using RT-qPCR. The diagnostic value of these miRNAs was explored using Receiver Operating Characteristic curve analysis. Pearson or Spearman correlation analysis was performed to assess the correlation between miRNAs and clinical indicators in SSc patients. Potential target genes of hsa-miR-155-3p and hsa-miR-155-5p were predicted using miRDB, Targetscan, and miRDIP databases. GO functional annotation, KEGG pathway enrichment analysis, protein-protein interaction network construction, and selection of central genes were conducted. Results: The expression levels of hsa-miR-155-3p and hsa- miR-155-5p were significantly higher in PBMCs of SSc patients compared to healthy controls (P<0.001). The ROC curve analysis showed that hsa-miR-155-3p and hsa-miR-155-5p had a high diagnostic value for SSc (AUC=1, P<0.001). Correlation analysis revealed that hsa- miR-155-3p, hsa-miR-155-5p, and clinical indicators such as high-resolution CT, neutrophil percentage, lymphocyte percentage, and albumin to globulin ratio were correlated (P<0.05). The signaling pathways enriched with target genes of hsa-miR-155-3p and hsa-miR-155- 5p were closely associated with the occurrence and development of SSc fibrosis, immunity, and inflammation. Conclusions: hsa-miR-155-3p and hsa-miR-155-5p may be involved in regulating the occurrence and development of SSc fibrosis, immunity, and inflammation. They have the potential to serve as biomarkers for clinical diagnosis and treatment of SSc.

非洲猪瘟:传染源和传播途径研究进展与分析

自2018年8月以来,非洲猪瘟在我国持续发生,给我国养猪业造成了严重的经济损失。目前,世界范围内尚未研制成功安全有效的非洲猪瘟疫苗,因此,对其防控主要依赖于消灭传染源和通过严格的生物安全措施切断传播途径。非洲猪瘟病毒的组织嗜性广泛,对外界环境抵抗力顽强,在各种猪源产品、节肢动物和受污染的环境中均可长期保持感染性。非洲猪瘟的传染源和传播途径多而复杂,大大增加了其防控难度。因此,对该病毒的传染源和传播途径进行系统的整理分析,对于及时发现和消灭传染源,切断传播途径,阻止非洲猪瘟疫情扩散,达到有效防控和最终根除具有重要意义。基于此,本文系统总结分析了非洲猪瘟病毒的传染源和传播途径,以期为该病的流行病学研究和防控提供重要参考。

重大动物疫病现场快速诊断检测与远程监测预警平台的建立与应用

为更好地推动动物疫病的防控与净化措施,摸清我国边境地区的主要动物疫病流行情况,拟建立动物疫病现场快速诊断与远程监测平台,对新疆喀什-哈萨克斯坦边境、昌吉-外蒙古边境、伊犁-哈萨克斯坦边境等地区的规模场、种畜场、屠宰场以及散养户的1254份羊血清、728份牛血清进行小反刍兽疫病毒抗体、口蹄疫病毒3ABC病原抗体、布鲁菌抗体的血清学检测。结果表明,共检出小反刍兽疫病原抗体阳性1137份,阳性率为90.67%;检出牛口蹄疫病毒抗体阳性血清6份,阳性率为0.87%;检出羊口蹄疫病毒抗体阳性血清13份,阳性率为1.06%;检出牛布鲁菌病病原抗体阳性血清372份,阳性率为51.1%;检出羊布鲁菌病病原抗体阳性血清724份,阳性率为57.74%。将上述检测方法与国外诊断试剂盒进行比对,结果表明使用的现场快速诊断检测试剂敏感性与特异性复合率较高,成功开发建立了动物疫病现场快速诊断与远程监测平台,探索了我国跨境地区种畜禽场动物疫情监测新模式,为我国边境地区的养殖场动物疫病防控净化机制建设提供数据支持与技术支撑。

卒中中心超早期康复治疗对急性脑出血并发症及康复效果的影响

目的:探究卒中中心超早期康复治疗对急性脑出血并发症患者及康复效果的影响。方法:选取涟水县人民医院康复医学科2018年1月—2019年12月收治的40例急性脑出血患者,按照随机数表法分为卒中中心病房组(卒中中心超早期康复治疗方法)和普通病房组(常规时期康复治疗方法),每组各20例,观察入院及结束治疗时神经功能缺损(NIHSS)评分和日常生活能力(ADL)、Barthel指数评分、住院期间感染情况及死亡情况、治疗有效率、并发症发生情况及住院时间。结果:卒中中心病房组治疗有效率为90.0%,普通病房组治疗有效率为70.0%,卒中中心病房组并发症发生率10.0%,普通病房组并发症发生率为30.0%,差异有统计学意义(χ^(2)=4.329、4.800,P<0.05)。两组患者康复治疗前NIHSS评分和ADL Barthel指数评分差异无统计学意义(P>0.05),康复治疗后卒中中心病房NIHSS评分低于普通病房组,卒中中心病房ADL Barthel指数评分高于普通病房组,差异有统计学意义(t=2.877、2.074,P<0.05)。卒中中心病房组泌尿道感染、肺部感染例数低于普通病房组,差异有统计学意义(χ^(2)=4.800、4.329,P<0.05),两组患者脑部直接原因死亡例数相同,卒中中心病房组非脑部直接原因死亡例数低于普通病房组,差异有统计学意义(χ^(2)=0.000、4.444,P<0.05)。卒中中心病房组患者平均住院时间短于普通病房组,差异有统计学意义(P<0.05)。结论:卒中中心模式下超早期康复训练患者康复效果好,并发症少,有利于急性脑出血患者的恢复。