The freshwater species,Euplotes mazeii n.sp.,and the marine species,E.balteatus(Dujardin,1841)Diesing,1850 were collected from China and investigated based on their living morphology,ciliary pattern,and small subunit ...The freshwater species,Euplotes mazeii n.sp.,and the marine species,E.balteatus(Dujardin,1841)Diesing,1850 were collected from China and investigated based on their living morphology,ciliary pattern,and small subunit ribosomal RNA(SSU rRNA)gene sequence data.Euplotes mazeii n.sp.was characterized by its small cell size((40-55)μm×(25-35)μm),nine frontoventral cirri,one marginal and two caudal cirri,seven dorsal kineties,and a double-eurystomus type of dorsal silverline system.Phylogenetic analyses based on molecular data confirmed the branching position of this new taxa within the genus Euplotes,with 5.01%(84 nucleotides)sequence variation,supporting the identification of a new species.We re-examined all described and supposedly conspecific populations and give a review of Euplotes balteatus.E.balteatus has been reported frequently;however,E.balteatus sensu Agamaliev,1968 is different from other populations in the number of marginal cirri or the type of silverline system,which suggests there might be a misidentification.The SSU rRNA gene sequences of two populations of E.balteatus from the studies of Chen et al.(2013)and Pan et al.(2012)differ from each other by more than 135 nucleotides,implying that the identity of the species currently associated with the SSU rRNA gene sequence in molecular databases requires further confirmation.展开更多
The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana(Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infracil...The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana(Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infraciliature and the small subunit(SSU) ribosomal RNA gene(r RNA) sequence. The new species, M. parastruederkypkeae n. sp. was identified according to its characteristics: body shape ellipsoidal, size about(165–200) ×(45–60) μm in vivo, cell color reddish; two types of cortical granules including wheat grain-like and yellow-greenish larger ones along the marginal cirri rows and dorsal kineties and dot-like and reddish smaller ones, grouped around marginal cirri on ventral side and arranged in short lines on dorsal side; 26–41 adoral membranelles; three frontal and one parabuccal, five to seven frontoterminal, one buccal, and three to six transverse cirri; seven to thirteen midventral pairs; five to nine unpaired ventral cirri, five to seven left and three to five right marginal rows; and three complete dorsal kineties. Phylogenetic analysis based on SSU r DNA sequences showed that both Metaurostylopsis and Neourostylopsis are monophyletic. As the internal relationship between and within both genera are not clear, further studies on the species in these two genera are necessary. The key characteristics of all known twelve Metaurostylopsis-Apourostylopsis-Neourostylopsis species complex were updated.展开更多
基金supported by the National Natural Science Foundation of China(Nos.32030015,32111530116)the China Postdoctoral Science Foundation Grant(No.2021M701993)the Fundamental Research Funds for the Central Universities(No.GK202207019)。
文摘The freshwater species,Euplotes mazeii n.sp.,and the marine species,E.balteatus(Dujardin,1841)Diesing,1850 were collected from China and investigated based on their living morphology,ciliary pattern,and small subunit ribosomal RNA(SSU rRNA)gene sequence data.Euplotes mazeii n.sp.was characterized by its small cell size((40-55)μm×(25-35)μm),nine frontoventral cirri,one marginal and two caudal cirri,seven dorsal kineties,and a double-eurystomus type of dorsal silverline system.Phylogenetic analyses based on molecular data confirmed the branching position of this new taxa within the genus Euplotes,with 5.01%(84 nucleotides)sequence variation,supporting the identification of a new species.We re-examined all described and supposedly conspecific populations and give a review of Euplotes balteatus.E.balteatus has been reported frequently;however,E.balteatus sensu Agamaliev,1968 is different from other populations in the number of marginal cirri or the type of silverline system,which suggests there might be a misidentification.The SSU rRNA gene sequences of two populations of E.balteatus from the studies of Chen et al.(2013)and Pan et al.(2012)differ from each other by more than 135 nucleotides,implying that the identity of the species currently associated with the SSU rRNA gene sequence in molecular databases requires further confirmation.
基金supported by the National Natural Science Foundation of China (Nos. 31572230, 31401963)he Ningbo Science Foundation (Nos. 2015A610263, 2015A610264)+2 种基金the Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University (xkzsc1413)the Scientific Research Fund of Zhejiang Provincial Education Department (pd2013102)the K.C.Wong Magna Fund in Ningbo University
文摘The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana(Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infraciliature and the small subunit(SSU) ribosomal RNA gene(r RNA) sequence. The new species, M. parastruederkypkeae n. sp. was identified according to its characteristics: body shape ellipsoidal, size about(165–200) ×(45–60) μm in vivo, cell color reddish; two types of cortical granules including wheat grain-like and yellow-greenish larger ones along the marginal cirri rows and dorsal kineties and dot-like and reddish smaller ones, grouped around marginal cirri on ventral side and arranged in short lines on dorsal side; 26–41 adoral membranelles; three frontal and one parabuccal, five to seven frontoterminal, one buccal, and three to six transverse cirri; seven to thirteen midventral pairs; five to nine unpaired ventral cirri, five to seven left and three to five right marginal rows; and three complete dorsal kineties. Phylogenetic analysis based on SSU r DNA sequences showed that both Metaurostylopsis and Neourostylopsis are monophyletic. As the internal relationship between and within both genera are not clear, further studies on the species in these two genera are necessary. The key characteristics of all known twelve Metaurostylopsis-Apourostylopsis-Neourostylopsis species complex were updated.
