Objective: To study taurine resist lead impact ability of learning and memory. Methods: Using NADPH - dhistochemistry method to study the quantity change of the rat's NOS positive neuron in hippocampus , the rat i...Objective: To study taurine resist lead impact ability of learning and memory. Methods: Using NADPH - dhistochemistry method to study the quantity change of the rat's NOS positive neuron in hippocampus , the rat in experi-ment sections which are feeded with distinct dosage lead acetate in drinking (0.02, 0.2g/L) and feed contain distinctdosage taurine (5, 10g/kg). Results: Taurine could increase NOS positive neuron quantity obviously in hippocampus ofrat induced lead lesion. Conclusion: Taurine could resist lead impact ability of learning and memory obviously.展开更多
文摘Objective: To study taurine resist lead impact ability of learning and memory. Methods: Using NADPH - dhistochemistry method to study the quantity change of the rat's NOS positive neuron in hippocampus , the rat in experi-ment sections which are feeded with distinct dosage lead acetate in drinking (0.02, 0.2g/L) and feed contain distinctdosage taurine (5, 10g/kg). Results: Taurine could increase NOS positive neuron quantity obviously in hippocampus ofrat induced lead lesion. Conclusion: Taurine could resist lead impact ability of learning and memory obviously.
文摘【目的】以细菌脂多糖(lipopolysaccharide,LPS)诱导MRL/lpr小鼠建立系统性红斑狼疮(systemic lupus erythematosus,SLE)动物模型,并对模型进行验证分析。【方法】16周龄MRL/lpr小鼠(雌、雄各半)随机分为两组:模型组单次腹腔注射LPS,对照组单次腹腔注射等量生理盐水。每周监测小鼠尿蛋白,4周后处死动物,计算脾指数,检测肌酐、尿素氮、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)等含量,ELISA法定量测定小鼠血清中抗双链DNA(double strand DNA,dsDNA)抗体水平,苏木素伊红(hematoxylin,HE)染色观察小鼠肾脏组织形态学变化。【结果】与对照组相比,模型组MRL/lpr小鼠脾脏均明显增大,尿蛋白等肾功指标升高,肾脏损伤严重;此外,LDL-C水平显著增加,HDL-C水平明显降低(P<0.01)。【结论】用LPS腹腔注射的MRL/lpr小鼠可表现出明显的SLE样病变,且发病程度较正常自发MRL/lpr小鼠严重,为后续的研究工作提供了一定的基础。
