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海南岛岸礁澄黄滨珊瑚(Porites lutea)集合种群的遗传结构和连通性
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作者 付成冲 李福宇 +5 位作者 陈丹丹 侯敬 王珺 李元超 王道儒 王嫣 《热带海洋学报》 CAS CSCD 北大核心 2023年第2期64-77,共14页
海南岛岸礁的造礁珊瑚代表性种类澄黄滨珊瑚(Porites lutea)是环境适应性较强的块状产卵型珊瑚。探究其遗传结构和连通性将有助于揭示其海南岛岸礁集合种群的遗传多样性格局和幼虫迁移路径,进而阐明海南珊瑚礁的恢复潜力。文章通过11个P... 海南岛岸礁的造礁珊瑚代表性种类澄黄滨珊瑚(Porites lutea)是环境适应性较强的块状产卵型珊瑚。探究其遗传结构和连通性将有助于揭示其海南岛岸礁集合种群的遗传多样性格局和幼虫迁移路径,进而阐明海南珊瑚礁的恢复潜力。文章通过11个P.lutea微卫星标记来分析10个海南岛岸礁地理群体(八所、海尾、大铲礁、邻昌礁、雷公岛、木栏头、铜鼓岭、龙湾、大洲岛和鹿回头)和1个西沙群岛(西沙七连屿)群体的遗传结构。结果显示,整体上各群体的遗传多样性中等偏低,平均等位基因丰度(allelic richness,R_(s))为(2.8±1.3)(八所群体)~(3.7±1.7)(邻昌礁群体),平均观测杂合度和期望杂合度分别为0.31(铜鼓岭群体)~0.54(大铲礁群体)和0.50(雷公岛群体)~0.64(海尾群体)。除海南岛东部龙湾群体、大洲岛群体和西部的八所群体、大铲礁群体之外,其他7/11的地理群体均呈杂合子缺失。海南岛岸礁澄黄滨珊瑚P.lutea地理群体间的遗传分化显示,集合种群分为北东南遗传连通带和西岸两支,支间遗传分化显著,前者包括北岸的雷公岛群体和木栏头群体、东岸的大洲岛群体,以及南岸的鹿回头和八所群体。由于珊瑚幼虫随海流迁移而形成的有效的基因流,消弭了它们之间的遗传分化,而西岸群体因北部湾沿岸海流交换不畅,与外部的基因交流受阻。西岸的海尾群体与西南部的八所群体间尽管相距不足50km,但遗传分化明显,这可能是由于昌化江径流形成的盐度波动和悬浮沉积物的隔离作用。同样,铜鼓岭群体因处于铜鼓岭岬角内波影区,其南部八门湾径流或限制了它与东岸群体的基因交流,因而呈现近交、低杂合度和非随机交配特性。此外,由于对离岸礁坡环境的趋同适应,东岸龙湾群体与西岸的离岸岛礁邻昌礁群体、大铲礁群体和海尾群体之间的遗传相似性更高。以P.lutea集合种群为代表的海南岛珊瑚岸礁有着相对充沛的基因交流,且因受海流、径流及复杂岸礁环境影响而形成了多层次的遗传分化和基因流格局,这构成其应对外界胁迫的主要自然恢复力。西沙群体与海南岛岸礁群体之间的遗传分化较大,呈现显著的地理隔离。西沙七连屿可能由于自身珊瑚礁健康状况的衰退而丧失了对海南岛珊瑚幼虫的补充能力。 展开更多
关键词 澄黄滨珊瑚 遗传连通性 微卫星标记 径流 岸礁结构 海流
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基于Landsat8遥感数据的西沙群岛永乐环礁底质分类与变化分析
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作者 李天驰 王道儒 +1 位作者 赵亮 凡仁福 《自然资源遥感》 CSCD 北大核心 2023年第2期70-79,共10页
在海-气环境变化剧烈的今日,准确高效地实现珊瑚礁底质信息识别是进行珊瑚礁动态监测研究的基础。文章获取了2013—2021年4个时期西沙群岛永乐环礁的Landsat8卫星数据,结合不同底质的光谱和纹理差异,提出了一种基于光谱纹理指数的决策... 在海-气环境变化剧烈的今日,准确高效地实现珊瑚礁底质信息识别是进行珊瑚礁动态监测研究的基础。文章获取了2013—2021年4个时期西沙群岛永乐环礁的Landsat8卫星数据,结合不同底质的光谱和纹理差异,提出了一种基于光谱纹理指数的决策树分类模型,采用面向对象和基于像元的分类方法进行珊瑚信息提取,并定量统计了永乐环礁底质变化情况。结果表明:面向对象的分类结果整体上优于基于像素的分类结果,且决策树分类结果的Kappa系数在0.631~0.681范围,分类精度高于传统监督分类精度约7~10个百分点;珊瑚丛生带大多分布在岛礁的中部水动力较弱区域,除银屿和金银岛上的珊瑚呈面状分布外,其他岛礁上的珊瑚多呈带状分布;总体时段内永乐环礁的珊瑚丛生带和沙洲面积变化显著,虽然珊瑚丛生带的总面积增加了1.689 km^(2),但石屿、晋卿岛、全富岛、珊瑚岛和羚羊礁的珊瑚丛生带退化情况严重,其面积减少了0.107~0.892 km^(2)不等。该文证明了利用中等空间分辨率影像建立的底质指数是可靠的,可应用于珊瑚遥感信息提取,能够为珊瑚礁资源调查及科学管理提供技术支持。 展开更多
关键词 遥感 永乐环礁 光谱特征 决策树 面向对象分类
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海南东寨港海水和沉积物中抗生素抗性基因污染特征研究 被引量:21
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作者 姜春霞 黎平 +4 位作者 李森楠 刁晓平 黄炜 王道儒 叶翠杏 《生态环境学报》 CSCD 北大核心 2019年第1期128-135,共8页
抗生素滥用及其诱导产生的抗生素抗性基因(Antibioticresistancegenes,ARGs)污染已经引起各界的广泛关注。东寨港是海南省重要的滩涂养殖基地,其ARGs的产生、传播和富集都可能对人类及生态健康产生危害。采集该区域海水和沉积物样本各10... 抗生素滥用及其诱导产生的抗生素抗性基因(Antibioticresistancegenes,ARGs)污染已经引起各界的广泛关注。东寨港是海南省重要的滩涂养殖基地,其ARGs的产生、传播和富集都可能对人类及生态健康产生危害。采集该区域海水和沉积物样本各10个,利用LC-MS/MS对海水和沉积物中磺胺类、四环素类、氯霉素类和喹诺酮类等4类15种抗生素进行测定,同时采用RT-PCR阐明了相应的12种ARGs(sul1、sul2、dfr A1;tet A、tet C、tet G、tet M;cata1、cata2、cmle1、cmle3;qnr S)及16S rRNA的分布特征,并对抗生素和ARGs的相关性进行分析。结果显示,所测ARGs在各沉积物样品中的检出率均为100%,海水样品中ARGs的检出率在80%-100%之间。其中,qnr S的检出率最高,存在于所有海水和沉积物样品中,而sul2的检出丰度最高。比较海水和沉积物中ARGs的相对丰度,发现沉积物中ARGs的污染程度高于海水。样品中抗生素总体检出率较低,其中沉积物仅有喹诺酮及磺胺类抗生素被检出,海水中除磺胺类抗生素外其他均未检出。与其他研究相比,该区域ARGs检出水平较高,而抗生素检出水平较低,ARGs含量与抗生素浓度相关性较弱(P>0.05),这说明由历史背景诱导产生的ARGs可进行自我扩增而持续存在于环境中。该研究结果可为当地抗生素的安全使用和ARGs的环境风险评价提供重要依据。 展开更多
关键词 沉积物 海水 抗生素残留 抗生素抗性基因 东寨港
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永兴岛及七连屿造礁石珊瑚近10年变化分析 被引量:17
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作者 李元超 陈石泉 +4 位作者 郑新庆 蔡泽富 吴钟解 王道儒 兰建新 《海洋学报》 CAS CSCD 北大核心 2018年第8期97-109,共13页
根据2007—2016年珊瑚礁监测数据,永兴岛及七连屿共调查到造礁石珊瑚11科113种,常见种有鹿角杯形珊瑚Pocillopora damicornis、疣状杯形珊瑚Pocillopora Verrucosa、粗野鹿角珊瑚Acropora humilis、澄黄滨珊瑚Porites lutea、丛生盔形珊... 根据2007—2016年珊瑚礁监测数据,永兴岛及七连屿共调查到造礁石珊瑚11科113种,常见种有鹿角杯形珊瑚Pocillopora damicornis、疣状杯形珊瑚Pocillopora Verrucosa、粗野鹿角珊瑚Acropora humilis、澄黄滨珊瑚Porites lutea、丛生盔形珊瑚Galaxea fascicularis、梳状菊花珊瑚Goniastrea pectinata及锯齿刺星珊瑚Cyphastrea serailia等。研究区域内造礁石珊瑚种类近10年(2007—2016年)来下降明显,其中永兴岛从39种下降到18种,下降率为53.85%;北岛从23种下降到17种,下降率为26.09%;赵述岛从46种下降到12种,下降率为73.91%;西沙洲从51种下降到15种,下降率为70.59%。群体形态群落中皮壳状种类数最为稳定,分枝类下降了59.26%;叶片状下降了75.00%;圆盘状下降了87.50%;团块状下降了57.14%。通过对研究区域造礁石珊瑚种类组成及群落形态等方面的变化分析可见,人为活动、长棘海星、珊瑚病害、台风及全球升温等因素逐渐影响到西沙海域的珊瑚礁生态系统。 展开更多
关键词 海南岛 永兴岛 七连屿 造礁石珊瑚 变化趋势
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Mean water level setup/setdown in the inlet-lagoon system induced by tidal action—a case study of Xincun Inlet,Hainan Island in China 被引量:7
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作者 GONG Wenping SHEN Jian wang daoru 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2008年第5期63-80,共18页
With the tides propagating from the open sea to the lagoon, the mean water level (MWL) in the inlet and lagoon becomes different from that at the open sea, and a setup/setdown is generated. The change of MWL (setup... With the tides propagating from the open sea to the lagoon, the mean water level (MWL) in the inlet and lagoon becomes different from that at the open sea, and a setup/setdown is generated. The change of MWL (setup/setdown) in the system imposes a great impact on regulating the development of tidal marshes, on determining the long-term water level for harbor maintenance, on the planning for the water front development with the flood control for the possible inundation, and on the interpretation of the historical sea level change when using tidal marsh peat deposits in the lagoon as the indicator for open sea' s sea level. In this case study on the mechanisms which control the setup/setdown in Xincun Inlet, Hainan in China, the 2-D barotropic mode of Eulerian - Lagrangian CIRCulation (ELCIRC) model was utilized. After model calibration and verification, a series of numerical experiments were conducted to examine the effects of bottom friction and advection terms, wetting and drying of intertidal areas, bathymetry and boundary conditions on the setup/setdown in the system. The modeling results show that setup occurs over the inlet and lagoon areas with an order of one tenth of the tide range at the entrance. The larger the bottom friction is, a larger setup is generated. Without the advection term, the setup is reduced clue to a decrease of water level gradient to compensate for the disappearance of the advection term. Even without overtides, a setup can still be developed in the system. Sea level rise and dredging in the inlet and tidal channel can cause a decrease of setup in the system, whereas shoaling of the system can increase the setup. The uniqueness of the Xincun Inlet with respect to MWL change is that there is no evident setdown in the inlet, which can be attributed to the complex geometry and bathymetry associated with the inlet system. 展开更多
关键词 tidal inlet water level setup/setdown ELCIRC Hainan Island
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The Characteristics of Storm Wave Behavior and Its Effect on Cage Culture Using the ADCIRC+SWAN Model in Houshui Bay, China 被引量:2
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作者 YIN Chao HUANG Haijun +2 位作者 wang daoru LIU Yanxia GUO Ziyue 《Journal of Ocean University of China》 SCIE CAS CSCD 2020年第2期307-319,共13页
The current storm wave hazard assessment tends to rely on a statistical method using wave models and fewer historical data which do not consider the effects of tidal and storm surge.In this paper,the wave-current coup... The current storm wave hazard assessment tends to rely on a statistical method using wave models and fewer historical data which do not consider the effects of tidal and storm surge.In this paper,the wave-current coupled model ADCIRC+SWAN was used to hindcast storm events in the last 30 years.We simulated storm wave on the basis of a large set of historical storms in the North-West Pacific Basin between 1985 and 2015 in Houshui Bay using the wave-current coupled model ADCIRC+SWAN to obtain the storm wave level maps.The results were used for the statistical analysis of the maximum significant wave heights in Houshui Bay and the behavior of wave associated with storm track.Comparisons made between observations and simulated results during typhoon Rammasun(2014)indicate agreement.In addition,results demonstrate that significant wave height in Houshui Bay is dominated by the storm wind velocity and the storm track.Two groups of synthetic storm tracks were designed to further investigate the worst case of typhoon scenarios.The storm wave analysis method developed for the Houshui Bay is significant in assisting government's decision-making in rational planning of deep sea net-cage culture.The method can be applied to other bays in the Hainan Island as well. 展开更多
关键词 storm wave ADCIRC+SWAN maximum significant wave height Houshui Bay deep sea net-cage
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Coral Ba/Ca and Mn/Ca Ratios as Proxies of Precipitation and Terrestrial Input at the Eastern Offshore Area of Hainan Island
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作者 JIANG Qiaowen CAO Zhimin +3 位作者 wang daoru LI Yuanchao WU Zhongjie NI Jianyu 《Journal of Ocean University of China》 SCIE CAS CSCD 2017年第6期1072-1080,共9页
Geochemical ratios in coral reef skeletons could be used as proxies to reconstruct past climatological and environmental records in data-poor regions. Using a 103-year data set(1902 to 2005), the annual variations in ... Geochemical ratios in coral reef skeletons could be used as proxies to reconstruct past climatological and environmental records in data-poor regions. Using a 103-year data set(1902 to 2005), the annual variations in Ba/Ca and Mn/Ca ratios of Porites lutea skeletons at an eastern offshore area of Hainan Island(19?12'28.4''N, 110?37'38.8''E) were analyzed using inductively coupled plasma-optic emission spectrometry(ICP-OES). The analysis results showed that Ba/Ca ratios varied from a minimum of 3.120 μmol mol^(-1) in 1903 to a maximum of 10.064 μmol mol^(-1) in 1944, with an average of 5.256 μmol mol^(-1). Mn/Ca ratios varied from 0.206 to 5.708 μmol mol^(-1) with an annual average of 1.234 μmol mol^(-1), with peak values in 2001, 1964 and 1932, that correlated with strong rainfall events caused by typhoons. Variation in Ba/Ca and Mn/Ca ratios were compared with available river discharge and precipitation records, providing insight into past climatological events. Human activities and their indirect effects could impact the strength of the relationship between Ba/Ca and Mn/Ca ratios and observed precipitation and terrestrial input in the future. 展开更多
关键词 Porites lutea eastern offshore area of Hainan Island Ba/Ca and Mn/Ca ratios TERRIGENOUS INPUT PRECIPITATION climate proxy records
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澄黄滨珊瑚微卫星标记的开发 被引量:2
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作者 李福宇 陈丹丹 +4 位作者 李元超 李世尧 侯敬 王道儒 王嫣 《基因组学与应用生物学》 CAS CSCD 北大核心 2021年第7期2513-2521,共9页
本研究通过挖掘GenBank中滨珊瑚属(Porites)近缘种的EST和基因组微卫星序列资源,使用种间扩增和重测序技术,开发了澄黄滨珊瑚(Porites lutea)-虫黄藻(Symbiotic zooxanthellae)共生体的微卫星标记,并通过纯的共生虫黄藻DNA的扩增验证,... 本研究通过挖掘GenBank中滨珊瑚属(Porites)近缘种的EST和基因组微卫星序列资源,使用种间扩增和重测序技术,开发了澄黄滨珊瑚(Porites lutea)-虫黄藻(Symbiotic zooxanthellae)共生体的微卫星标记,并通过纯的共生虫黄藻DNA的扩增验证,确认了20个澄黄滨珊瑚微卫星标记和43个共生虫黄藻微卫星标记。在海南岛岸礁八所群体(BS,n=16)中测试扩增,有19个标记呈现多态性。多态位点的等位基因数为2~7(平均3.5±1.8),观测杂合度(H_(o))和期望杂合度(H_(e))分别为0.06~0.94(平均0.34±0.29)和0.18~0.83(平均0.57±0.20)。由于存在无效等位基因,4个位点在经Bonferroin多重比较校正后仍然偏离Hardy-Weinberg平衡(P_(HWE)<0.05)。这是澄黄滨珊瑚的首套微卫星标记,它们可用于对该物种遗传多样性和连通性的研究,从而促进印度洋-太平洋海区濒危的珊瑚礁生态系统及造礁珊瑚群落的保护。 展开更多
关键词 澄黄滨珊瑚 微卫星标记 遗传连通性 虫黄藻
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Coral cover as a proxy of disturbance:A case study of the biodiversity of the hermatypic corals in Yongxing Island, Xisha Islands in the South China Sea 被引量:4
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作者 HUANG Hui LIAN Jiansheng +3 位作者 HUANG Xiaoping HUANG Liangmin ZOU Renlin wang daoru 《Chinese Science Bulletin》 SCIE EI CAS 2006年第B12期129-135,共7页
The Yongxing Island is the biggest island of Xisha Islands in the middle part of the South China Sea. It occupies 1.8 square kilometers of land area and has permanent residence on it. There are only a few papers on th... The Yongxing Island is the biggest island of Xisha Islands in the middle part of the South China Sea. It occupies 1.8 square kilometers of land area and has permanent residence on it. There are only a few papers on the coral community of the Xisha Islands, reporting the species composition, structure and zonations of the hermatypic coral community in 1970s. The present study describes the hermatypic coral community based on the quadrat survey after almost 30 years in Yongxing Island in August 2002. It was the first time to present the percent cover data of live corals of Yongxing Island via a systematic scheme of quantitative quadrat sampling. The average total percent cover of the live coral is 68.4%. We found the total percent cover (TPC) could be an idea proxy of the overall disturbance regime impinging on the coral community. TPC can be used as an integrated measure of disturbance to coral reef. Using this surrogate, the best fit relation between TPC of the live corals and species richness, species diversity, or the species evenness is the unimodal second-order polymorphic parabola equation. This result is expected by the intermediate disturbance hypothesis (IDH). From the fitted parabola equations, we calculated the best TPCs corresponding to the maximal species richness, the highest species diversity, or the most evenness respectively. They are surprisingly stabilized between 55% and 58%. Coralcover is the basic parameter widely available for most observation, research or monitoring programs. The good qualitative characteristics of percent cover provide powerful tool for the experimental, theoretical and modeling studies of coral reef in response to the disturbance. 展开更多
关键词 南海 西沙群岛 永兴岛 造礁珊瑚 生物多样性 扰动
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