Continuous-wave chemical oxygen-iodine lasers (COILs) can be operated in a pulsed operation mode to obtain a higher peak power.The key point is to obtain a uniform and stable glow discharge in the mixture of singlet d...Continuous-wave chemical oxygen-iodine lasers (COILs) can be operated in a pulsed operation mode to obtain a higher peak power.The key point is to obtain a uniform and stable glow discharge in the mixture of singlet delta oxygen and iodide.We propose using an electrode system with the assistance of surface sliding pre-ionization to solve the problem of the stable glow discharge with a large aperture.The pre-ionization unit is symmetrically fixed on the plane of the cathode surface.A uniform and stable glow discharge is obtained in a mixture of iodide (such as CH3I) and nitrogen at the specific deposition energy of 4.5 J/L,pressure of 1.99-3.32 kPa,aperture size of 11 cm × 10cm.The electrode system is applied in a pulsed COIL.Laser energy up to 4.4J is obtained and the specific energy output is 2 J/L.展开更多
Sulfate can be activated by ATP sulfurylase and adenosine 5'-phosphosulfate kinase(APSK) in vivo. Recent studies suggested that APSK in Arabidopsis thaliana regulated the partition between APS reduction and phosph...Sulfate can be activated by ATP sulfurylase and adenosine 5'-phosphosulfate kinase(APSK) in vivo. Recent studies suggested that APSK in Arabidopsis thaliana regulated the partition between APS reduction and phosphorylation and its activity can be modulated by cellular redox status. In order to study regulation of APSK in rice(Os APSK), Os APSK1 gene was cloned and its activity was analyzed. Os APSK1 C36 and C69 were found to be the conserved counterparts of C86 and C119, which involved in disulfide formation in At APSK. C36A/C69 A Os APSK1 double mutation was made by site directed mutagenesis. Os APSK1 and its mutant were prokaryotically over-expressed and purified, and then assayed for APS phosphorylation activity. Os APSK1 activity was depressed by oxidized glutathione, while the activity of its mutant was not. Further studies in the case that oxidative stress will fluctuate in vivo 3'-phosphoadenosine-5'-phosphosulfate content, and all APSK isoenzymes have similar regulation patterns are necessary to be performed.展开更多
A model is developed that describes the temporal evolution of the sheath during a pulse of high negative voltage applied to a spherical and cylindrical target in a plasma such as that present in plasma source ion impl...A model is developed that describes the temporal evolution of the sheath during a pulse of high negative voltage applied to a spherical and cylindrical target in a plasma such as that present in plasma source ion implantation for the case in which the pressure of the neutraJ gas is large enough that the ion motion in the sheath can be assumed to be highly collisional.The analytic expressions of the sheath expanding velocity are obtained.The positions of the shea th edge as a function of time predicted by the model are in agreement with those obtained by Huid equations.展开更多
文摘Continuous-wave chemical oxygen-iodine lasers (COILs) can be operated in a pulsed operation mode to obtain a higher peak power.The key point is to obtain a uniform and stable glow discharge in the mixture of singlet delta oxygen and iodide.We propose using an electrode system with the assistance of surface sliding pre-ionization to solve the problem of the stable glow discharge with a large aperture.The pre-ionization unit is symmetrically fixed on the plane of the cathode surface.A uniform and stable glow discharge is obtained in a mixture of iodide (such as CH3I) and nitrogen at the specific deposition energy of 4.5 J/L,pressure of 1.99-3.32 kPa,aperture size of 11 cm × 10cm.The electrode system is applied in a pulsed COIL.Laser energy up to 4.4J is obtained and the specific energy output is 2 J/L.
基金funded by the Natural Science Foundation of China(Grant No.31070055)the Academic Climb Projects for Young Academic Leaders in Universities of Zhejiang Province(Grant No.pd2013060)+1 种基金the State Innovation and Entrepreneurship Projects for Undergraduate Students(Grant No.201410345017)the Open Research Foundation from Zhejiang Province Top Key Discipline of Biology(Grant No.KFJJ2014008)
文摘Sulfate can be activated by ATP sulfurylase and adenosine 5'-phosphosulfate kinase(APSK) in vivo. Recent studies suggested that APSK in Arabidopsis thaliana regulated the partition between APS reduction and phosphorylation and its activity can be modulated by cellular redox status. In order to study regulation of APSK in rice(Os APSK), Os APSK1 gene was cloned and its activity was analyzed. Os APSK1 C36 and C69 were found to be the conserved counterparts of C86 and C119, which involved in disulfide formation in At APSK. C36A/C69 A Os APSK1 double mutation was made by site directed mutagenesis. Os APSK1 and its mutant were prokaryotically over-expressed and purified, and then assayed for APS phosphorylation activity. Os APSK1 activity was depressed by oxidized glutathione, while the activity of its mutant was not. Further studies in the case that oxidative stress will fluctuate in vivo 3'-phosphoadenosine-5'-phosphosulfate content, and all APSK isoenzymes have similar regulation patterns are necessary to be performed.
基金Supported by the National Natural Science Foundation of Cliina.
文摘A model is developed that describes the temporal evolution of the sheath during a pulse of high negative voltage applied to a spherical and cylindrical target in a plasma such as that present in plasma source ion implantation for the case in which the pressure of the neutraJ gas is large enough that the ion motion in the sheath can be assumed to be highly collisional.The analytic expressions of the sheath expanding velocity are obtained.The positions of the shea th edge as a function of time predicted by the model are in agreement with those obtained by Huid equations.
