Ecdysteroids in the pupae of Opogona sacchari (Bojer), a new invasive alien pest

In order to understand the composition and quantitative variation of ecdysteriods in the pu- pae of Opogona sacchari (Bojer), a new invasive alien pest, we analyzed the pupal ecdysteroid com- position and titers in this pest. The main component of ecdysteriods in the pupae of O. sacchari is 20-hydroxyecdysone. The titre of ecdysteriods in both male pupae and female pupae get higher gradually, and then decreased slowly after reaching a peak (80 ng/pupa). The titre of ecdysteriods was about 30 ng/pupa in the later stage of pupae. The peak of male pupae appeared on the third day, but the peak of female pupae lasted from the second day to the fourth day during pupal developments. The ecdysteroid content was higher in female pupae than in male pupae during specific pupal time (the 1st, 2nd, 4th, 5th, 6th day). We also found that the titre of ec- dysteroids in posterior part of pupae was higher than in anterior part of pupae obviously.

Ecdysteroids in the larvae of Opogona sacchari(Bojer),a invasive alien pest

In order to understand the composition and quantitative variation of ecdysteriods in the larvae of Opogona sacchari (Bojer), a invasive alien pest, we analyzed the larval ecdysteroid composition and titers in this pest. The main component of ecdysteriods in the larvae of O. sacchari is 20-hydroxyecdysone, and also there is a little 26-hydroxyecdysone. The titer of ecdysteriods in the larvae from the 1st instar to the 7th instar was gotten higher gradually compared on ng ecdyster-oid/larva, but no regularity could be found about the titer if compared on ng ecdysteroid/g avoirdupois. There was only one peak of ecdysteroids (0.5475 ng/larva) showed at day 2 during the developmental time of the 6th instar larvae. However, there were two peaks appearing during the developmental time of the 7th instar larvae , one peak (0.29415 ng/larva) at day 3, another (0.214 ng/larva) at day 5.