目的:借助整合药理学平台研究当归补血汤治疗贫血的分子作用机制。方法:依托中医药整合药理学平台(integrative pharmacology-based research platform of traditional Chinese medicine,TCMIP)V2.0,构建当归补血汤治疗贫血的“方剂-中...目的:借助整合药理学平台研究当归补血汤治疗贫血的分子作用机制。方法:依托中医药整合药理学平台(integrative pharmacology-based research platform of traditional Chinese medicine,TCMIP)V2.0,构建当归补血汤治疗贫血的“方剂-中药材-成分-疾病-靶标-通路”多维关系网络图,预测其治疗贫血疾病的关键靶标和作用通路,揭示其分子作用机制。结果:当归补血汤中与贫血疾病相关的化学成分包括谷甾醇、叶酸、华良姜素、豆甾醇、琥珀酸、叶酸、癸二酸、壬二酸8种,核心靶标176个,共有靶标9个;前20位核心靶标和通路中多数靶标与癌性贫血发生有关,如Akt1、CCND1、ABL1、EPOR、GATA1、SMAD4等可能通过细胞增殖正调节、细胞增长负调节、蛋白激酶b信号蛋白正调节、细胞分化等通路起抑制癌细胞增殖、增长、分化及凋亡等作用;而NFKB1、HSP90AA1、TFRC、TF、NDUFV1、NDUFS1等靶标可能通过细胞因子介导的信号通路、Nf-kappab转录因子正调节、线粒体呼吸链组装、红细胞分化、促红细胞生成素介导信号通路、肽丝氨酸磷酸化正调节等途径,以提高免疫力、补充营养、消除炎症等方式治疗缺铁性贫血、炎性贫血、心脑缺血、糖尿病并发症及改善营养不良性等的贫血。结论:当归补血汤可通过多靶点、多通路治疗缺铁性贫血、心脑缺血、癌性贫血、炎性贫血、营养不良性贫血及糖尿病、高脂血症等引起的贫血。展开更多
High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd a...High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd accumulation in brown rice. Results showed that the exogenous ALA and EBR had no significant effects on agronomic traits, soil pH and total Cd content in soil, but had some effects on the available Cd content in soil, and significantly influenced the Cd accumulation in the different parts of rice. Results also showed that 100 mg/L exogenous ALA significantly reduced the Cd accumulation in brown rice to blow the food safety standard(0.2 mg/kg), and also significantly reduced the Cd contents in the roots and culm of rice. However, 200 mg/L exogenous ALA treatment increased the Cd content in brown rice remarkably. In addition, 0.15 mg/L EBR treatment increased Cd accumulation in roots, culm, leaves and brown rice notably, whereas 0.30 mg/L exogenous EBR treatment reduced the Cd accumulation in brown rice properly, but it was not significant. Therefore,proper concentration of ALA can effectively reduce the Cd accumulation in brown rice, which can be used as an effective technical method for the safe production of rice in Cd polluted farmland.展开更多
文摘目的:借助整合药理学平台研究当归补血汤治疗贫血的分子作用机制。方法:依托中医药整合药理学平台(integrative pharmacology-based research platform of traditional Chinese medicine,TCMIP)V2.0,构建当归补血汤治疗贫血的“方剂-中药材-成分-疾病-靶标-通路”多维关系网络图,预测其治疗贫血疾病的关键靶标和作用通路,揭示其分子作用机制。结果:当归补血汤中与贫血疾病相关的化学成分包括谷甾醇、叶酸、华良姜素、豆甾醇、琥珀酸、叶酸、癸二酸、壬二酸8种,核心靶标176个,共有靶标9个;前20位核心靶标和通路中多数靶标与癌性贫血发生有关,如Akt1、CCND1、ABL1、EPOR、GATA1、SMAD4等可能通过细胞增殖正调节、细胞增长负调节、蛋白激酶b信号蛋白正调节、细胞分化等通路起抑制癌细胞增殖、增长、分化及凋亡等作用;而NFKB1、HSP90AA1、TFRC、TF、NDUFV1、NDUFS1等靶标可能通过细胞因子介导的信号通路、Nf-kappab转录因子正调节、线粒体呼吸链组装、红细胞分化、促红细胞生成素介导信号通路、肽丝氨酸磷酸化正调节等途径,以提高免疫力、补充营养、消除炎症等方式治疗缺铁性贫血、炎性贫血、心脑缺血、糖尿病并发症及改善营养不良性等的贫血。结论:当归补血汤可通过多靶点、多通路治疗缺铁性贫血、心脑缺血、癌性贫血、炎性贫血、营养不良性贫血及糖尿病、高脂血症等引起的贫血。
基金supported by the Natural Science Foundation of Zhejiang Province,China(Grant No.LY17C020005)the Key Research and Development Project of Zhejiang Province,China(Grant No.2015C03020-4)+2 种基金the National Nature Science Foundation of China(Grant No.31401356)Jinhua Science and Technology Project(Grant No.2015-2-012)the National Training Program for College Students to Innovate and Start Enterprise(Grant No.201710356013)
文摘High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd accumulation in brown rice. Results showed that the exogenous ALA and EBR had no significant effects on agronomic traits, soil pH and total Cd content in soil, but had some effects on the available Cd content in soil, and significantly influenced the Cd accumulation in the different parts of rice. Results also showed that 100 mg/L exogenous ALA significantly reduced the Cd accumulation in brown rice to blow the food safety standard(0.2 mg/kg), and also significantly reduced the Cd contents in the roots and culm of rice. However, 200 mg/L exogenous ALA treatment increased the Cd content in brown rice remarkably. In addition, 0.15 mg/L EBR treatment increased Cd accumulation in roots, culm, leaves and brown rice notably, whereas 0.30 mg/L exogenous EBR treatment reduced the Cd accumulation in brown rice properly, but it was not significant. Therefore,proper concentration of ALA can effectively reduce the Cd accumulation in brown rice, which can be used as an effective technical method for the safe production of rice in Cd polluted farmland.
