细辛脑中蓝色荧光杂质生成机理与结构鉴定

细辛脑原料药中含有一种有害的蓝色荧光杂质且难以去除,但关于其生成机理、分子结构、性质等相关研究尚未见报道。采用气相色谱法考察了α-细辛脑在不同溶剂中的蓝色荧光杂质含量的变化,探讨了蓝色荧光杂质生成条件和机理,合成了蓝色荧光杂质并通过核磁、红外光谱、质谱鉴定了其分子结构。结果表明:蓝色荧光杂质是2,4,5-三甲氧基苯甲醛,由空气氧化细辛脑生成,酸性介质具有催化作用。以细辛脑为原料,高碘酸钠水溶液作氧化剂,在乙酸-甲醇介质中合成了蓝色荧光杂质,产率为5.9%,熔点为111~113℃。

几种新型二噁英半抗原的合成

酶联免疫分析(ELISA)技术具有灵敏度高、操作简单及耗时少等特点,应用于二噁英类化合物的检测,可以解决目前通用的色谱/质谱联用法检测费用高、样品前处理繁琐、检测周期长等问题。相关研究表明,ELISA技术的关键是设计与目标分子在大小、形状(立体结构)和电子性能上尽量相似的半抗原分子。因此,设计并合成包含二苯并-对-二噁英类结构的二噁英半抗原是提高其灵敏度和准确性的关键。基于"二噁英半抗原中间体的合成研究",继续完成了二噁英成环关键步骤的反应条件探索,并且经水解最终得到4个未见报道的二噁英半抗原,同时对5个重要中间体、4个最终产物进行了核磁验证。

基于CRIO的智能型地铁列车能耗数据采集系统

为了对地铁列车能耗和节能问题进行研究,准确检测到列车牵引和供电系统能耗数据尤为重要。在此需求下,根据地铁列车各电气子系统的特点及能耗数据采集需求,阐述了自启动采集、变频率采样等智能采集关键技术,完成了基于NI CompactRIO采集设备、高精度多元传感器、信号调理箱等硬件和基于FPGA编程技术的软件系统设计,实现了对列车能耗参数的检测。试验结果表明,测试系统计算能耗与车载系统记录能耗值具有良好的一致性,能够实现对能耗参数的变频率采样和数据的自启动采集与存储。基于CRIO的智能型地铁列车能耗数据采集系统采集精度高、功耗低、实用性强,适用于地铁列车能耗数据的采集。

TMEM16A contributes to endothelial dysfunction through accelerating Nox2 NADPH oxidase-derived ROS generation in hypertension

OBJECTIVE The Ca2+-activated Cl-channel(Ca CC)plays a crucial role in various physiological functions.Recent evidences suggest TMEM16A encodes CaC C in various cells,including endothelial cells.However,the role of TMEM16A in the vascular endothelial dysfunction in hypertension is unclear.METHODS In the study,RT-PCR,Western blotting,co-immunopricipitation,confocal imaging,patch-clamp,and endothelial-specific TMEM16A transgenic and knockout mice were employed.RESULTS We found that TMEM16A was expressed abundantly and functioned as Ca CC in endothelial cells.AngiotensinⅡ(AngⅡ)induced endothelial dysfunction with an increase in TMEM16A expression,which was alleviated by TMEM16A inhibitor.Further studies revealed that TMEM16A endothelial-specific knockout significantly lowered the blood pressure and ameliorated endothelial dysfunction in AngⅡ-induced hypertension,whereas,TMEM16A endothelial-specific overexpression showed the opposite effects.These results were related to the increased reactive oxygen species(ROS)generation,NADPH oxidase activation,and Nox2,p22phox expression facilitated by TMEM16A upon AngⅡ-induced hypertensive challenges.Moreover,TMEM16A directly interacted with Nox2 monomer and reduced the degradation of Nox2 through the proteasome-dependent endoplasmic recticulum-associated degradation pathway.TMEM16A also potentiated the translocation of p47phox and p67phox from cytosol to cell membrane and the subsequent interaction with Nox2.CONCLUSION Our results demonstrated that TMEM16A,as Ca CC,is a positive regulator of ROS generation via upregulating the activation of Nox2 NADPH oxidase in the vascular endothelium,and therefore facilitates endothelial dysfunction and hypertension.Modification of TMEM16A may be a novel therapeutic strategy for endothelial dysfunction-associated cardiovascular diseases.

考虑酸蚀蚓孔区的酸压水平井产能研究

碳酸盐岩储层酸压过程中,由于酸液滤失作用,在生成的主裂缝壁面将形成酸蚀蚓孔,酸压改造后增产效果将很大程度上受到蚓孔区域的影响。在碳酸盐岩水平井分段压裂的基础上,考虑酸压蚓孔区域的存在,将蚓孔区域看作相互影响的基质-蚓孔双重介质系统,给出蚓孔区综合渗流数学方程,完成地层-蚓孔区-裂缝-水平井井筒的水平井酸压渗流数学模型的建立.将建立的方程组进行差分离散,编写程序,分析裂缝参数与蚓孔参数对产能的影响:1)酸压水平井产能随着裂缝条数、裂缝长度、裂缝导流能力的增加而增大,且各因素对产能影响程度依次减弱.2)酸压水平井产能随着蚓孔导流能力、蚓孔长度的增加而增大,且其中蚓孔长度对产能影响相对更大。