对铁线莲属(Clematis Linn.)植物基因组DNA的ISSR-PCR反应体系进行了优化,并采用ISSR分子标记对32个铁线莲属植物(包括8个野生种、1个变种和23个品种)及2个近缘属植物的遗传多样性进行研究。结果显示:铁线莲属植物基因组DNA ISSR-PCR最...对铁线莲属(Clematis Linn.)植物基因组DNA的ISSR-PCR反应体系进行了优化,并采用ISSR分子标记对32个铁线莲属植物(包括8个野生种、1个变种和23个品种)及2个近缘属植物的遗传多样性进行研究。结果显示:铁线莲属植物基因组DNA ISSR-PCR最佳反应体系的总体积为25.0μL,包括40 ng·μL^-1模板DNA 1.0μL、0.4μmol·L^-1引物1.0μL、0.15 mmol·L^-1dNTPs 2.0μL、10×Ex Taq Buffer(Mg2+ Plus) 2.0μL、TaKaRa Ex Taq酶0.2μL和ddH2O 18.8μL。对32个铁线莲属植物及2个近缘属植物基因组DNA的ISSR-PCR分析结果显示:共扩增出172个条带(位点),平均每条引物扩增出13.2个位点,其中,多态性位点168个,平均每条引物扩增出12.9个多态性位点,平均多态性位点百分率为98.2%。聚类分析结果显示:32个铁线莲属植物及2个近缘属植物的遗传距离在0.22~1.21之间。在遗传距离1.02处,将32个铁线莲属植物分为3个大组,‘杰出’(‘Superba’)单独聚为1个大组,‘斯托尔韦克’(‘Stolwijk Gold’)和‘柠檬之梦’(‘Lemon Dream’)聚为1个大组,其余植物聚为第3大组;在遗传距离0.92处,将第3大组进一步划分为3个亚组,第1亚组包括‘阿迪森’(‘Addisonii’)、‘帕斯卡’(‘Pascal’)和‘樱桃唇’(‘Cherry Lip’),第2亚组包括短柱铁线莲(C.cadmia Buch.-Ham. ex Wall.)、灰叶铁线莲[C.canescens(Turcz.) W. T. Wang et M. C. Chang]、毛蕊铁线莲(C.lasiandra Maxim.)、曲柄铁线莲(C.repens Finet et Gagnep.)、‘铃儿响叮当’(‘Jingle Bells’)和‘雀斑’(‘Freckles’),第3亚组包括吴兴铁线莲(C.huchouensis Tamura)等20个铁线莲属植物;在遗传距离0.77处,将第3亚组划分为7个小组。研究结果表明:ISSR分子标记能够应用于铁线莲属植物的遗传多样性分析。展开更多
[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their ef...[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall（ chitin and phenoloxidase）,the physiological and biochemical indices acting on the nervous system（ Acetylcholinesterase）,and the physiological and biochemical indices acting on the digestive system（ protease,amylase and lipase） of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.展开更多
文摘对铁线莲属(Clematis Linn.)植物基因组DNA的ISSR-PCR反应体系进行了优化,并采用ISSR分子标记对32个铁线莲属植物(包括8个野生种、1个变种和23个品种)及2个近缘属植物的遗传多样性进行研究。结果显示:铁线莲属植物基因组DNA ISSR-PCR最佳反应体系的总体积为25.0μL,包括40 ng·μL^-1模板DNA 1.0μL、0.4μmol·L^-1引物1.0μL、0.15 mmol·L^-1dNTPs 2.0μL、10×Ex Taq Buffer(Mg2+ Plus) 2.0μL、TaKaRa Ex Taq酶0.2μL和ddH2O 18.8μL。对32个铁线莲属植物及2个近缘属植物基因组DNA的ISSR-PCR分析结果显示:共扩增出172个条带(位点),平均每条引物扩增出13.2个位点,其中,多态性位点168个,平均每条引物扩增出12.9个多态性位点,平均多态性位点百分率为98.2%。聚类分析结果显示:32个铁线莲属植物及2个近缘属植物的遗传距离在0.22~1.21之间。在遗传距离1.02处,将32个铁线莲属植物分为3个大组,‘杰出’(‘Superba’)单独聚为1个大组,‘斯托尔韦克’(‘Stolwijk Gold’)和‘柠檬之梦’(‘Lemon Dream’)聚为1个大组,其余植物聚为第3大组;在遗传距离0.92处,将第3大组进一步划分为3个亚组,第1亚组包括‘阿迪森’(‘Addisonii’)、‘帕斯卡’(‘Pascal’)和‘樱桃唇’(‘Cherry Lip’),第2亚组包括短柱铁线莲(C.cadmia Buch.-Ham. ex Wall.)、灰叶铁线莲[C.canescens(Turcz.) W. T. Wang et M. C. Chang]、毛蕊铁线莲(C.lasiandra Maxim.)、曲柄铁线莲(C.repens Finet et Gagnep.)、‘铃儿响叮当’(‘Jingle Bells’)和‘雀斑’(‘Freckles’),第3亚组包括吴兴铁线莲(C.huchouensis Tamura)等20个铁线莲属植物;在遗传距离0.77处,将第3亚组划分为7个小组。研究结果表明:ISSR分子标记能够应用于铁线莲属植物的遗传多样性分析。
基金Supported by Open Fund of Key Laboratory of Contaminated Environment Con-trol and Regional Ecology Safety(SYU-KF-L-05)Science and TechnologyProject of Liaoning Province(2012214001)
文摘[Objective]The paper was to study the effects of asarum essential oils on physiological and biochemical indices of two agricultural pests. [Method]Asarum essential oils were extracted by different methods,and their effects on physiological and biochemical indices related to the structure of body wall（ chitin and phenoloxidase）,the physiological and biochemical indices acting on the nervous system（ Acetylcholinesterase）,and the physiological and biochemical indices acting on the digestive system（ protease,amylase and lipase） of Ostrinia furnacalis and Mythimna seperata were studied. [Result]Asarum essential oils had no effect no chitin content of body wall under the experimental concentration,and had no inhibitory effect but activated effect on polyphenol oxidase activity. Asarum essential oils could act on the nervous system of insect,and had strong inhibitory effect against acetylcholinesterase activity of O. furnacalis larvae,with inhibition rates of 24. 86%- 38. 79%. Asarum essential oils showed a significant activated effect on pepsin activity in midgut,but the activated amplitude became smaller with the prolongation of treatment time. Asarum essential oils showed a significant inhibitory effect on activities of amylase and lipase,indicating that it could play an insecticidal effect by inhibiting activity of amylase and lipase,and it could act on digestive system of insect. [Conclusion]The study laid the foundation for further development of asarum as botanical pesticide.
