目的探讨IL-6对HIV感染者CXCR5^(+)CD8^(+)T细胞[滤泡细胞毒性T细胞(follicular cytotoxic T cells,TFC)]数量及功能的影响。方法将2018年10月—2020年12月衡阳市第三人民医院就诊的32例未接受抗HIV治疗的HIV感染者纳入研究,在有或者无I...目的探讨IL-6对HIV感染者CXCR5^(+)CD8^(+)T细胞[滤泡细胞毒性T细胞(follicular cytotoxic T cells,TFC)]数量及功能的影响。方法将2018年10月—2020年12月衡阳市第三人民医院就诊的32例未接受抗HIV治疗的HIV感染者纳入研究,在有或者无IL-6刺激外周血单个核细胞或者TFC的情况下,通过体外培养对比分析TFC数量、功能和凋亡的差异。结果在IL-6刺激的情况下,TFC的数量增加、功能增强及凋亡水平降低。结论对于HIV感染者,IL-6具有增强TFC数量和功能的作用。展开更多
Heavy metal pollution has received increasing attention in recent years mainly because of the public awareness of environmental issues. In this study we have evaluated the effect of cadmium （Cd） on enzymes activity,...Heavy metal pollution has received increasing attention in recent years mainly because of the public awareness of environmental issues. In this study we have evaluated the effect of cadmium （Cd） on enzymes activity, substrate utilization pattern and diversity of microbial communities in soil spiked with 0, 20, 40, 60, 80, and 100 mg/kg Cd, during 60 d of incubation at 25℃. Enzyme activities determined at 0, 15, 30, 45, and 60 d after heavy metal application（DAA） showed marked declines for various Cd treatments, and up to 60 DAA, 100 mg/kg Cd resulted in 50.1%, 47.4%, and 39.8% decreases in soil urease, acid phosphatase and dehydrogenase activities, respectively to control. At 60 DAA, substrate utilization pattern of soil microbial communities determined by inoculating Biolog ECO plates indicated that Cd addition had markedly inhibited the functional activity of soil microbial communities and multivariate analysis of sole carbon source utilization showed significantly different utilization patterns for 80 and 100 mg/kg Cd treatments. The structural diversity of soil microbial communities assessed by PCR-DGGE method at 60 DAA, illustrated that DGGE patterns in soil simplified with increasing Cd concentration, and clustering of DGGE profiles for various Cd treatments revealed that they had more than 50% difference with that of control.展开更多
文摘目的探讨IL-6对HIV感染者CXCR5^(+)CD8^(+)T细胞[滤泡细胞毒性T细胞(follicular cytotoxic T cells,TFC)]数量及功能的影响。方法将2018年10月—2020年12月衡阳市第三人民医院就诊的32例未接受抗HIV治疗的HIV感染者纳入研究,在有或者无IL-6刺激外周血单个核细胞或者TFC的情况下,通过体外培养对比分析TFC数量、功能和凋亡的差异。结果在IL-6刺激的情况下,TFC的数量增加、功能增强及凋亡水平降低。结论对于HIV感染者,IL-6具有增强TFC数量和功能的作用。
基金The National Basic Research Programof China(2005CB121104) the National Key Project for Science and Technology of China(No.2001BA804A25andthe Natural Science Foundation of Zhejiang Province(Y504128)
文摘Heavy metal pollution has received increasing attention in recent years mainly because of the public awareness of environmental issues. In this study we have evaluated the effect of cadmium （Cd） on enzymes activity, substrate utilization pattern and diversity of microbial communities in soil spiked with 0, 20, 40, 60, 80, and 100 mg/kg Cd, during 60 d of incubation at 25℃. Enzyme activities determined at 0, 15, 30, 45, and 60 d after heavy metal application（DAA） showed marked declines for various Cd treatments, and up to 60 DAA, 100 mg/kg Cd resulted in 50.1%, 47.4%, and 39.8% decreases in soil urease, acid phosphatase and dehydrogenase activities, respectively to control. At 60 DAA, substrate utilization pattern of soil microbial communities determined by inoculating Biolog ECO plates indicated that Cd addition had markedly inhibited the functional activity of soil microbial communities and multivariate analysis of sole carbon source utilization showed significantly different utilization patterns for 80 and 100 mg/kg Cd treatments. The structural diversity of soil microbial communities assessed by PCR-DGGE method at 60 DAA, illustrated that DGGE patterns in soil simplified with increasing Cd concentration, and clustering of DGGE profiles for various Cd treatments revealed that they had more than 50% difference with that of control.
