Stripe rust, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14...Stripe rust, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 （a wheat cultivar susceptible to all Pst races identified in China）. The seedlings of the parents and F1 plants, Fz, F3 and BC1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat （SSR） markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection （MAS） in breeding programs for stripe rust.展开更多
With considering the interlayer mass transfer and fluid influx from the reservoir, a one-dimensional two-layer hydraulic model was established to describe the mechanism of transient cuttings transport with foam fluid ...With considering the interlayer mass transfer and fluid influx from the reservoir, a one-dimensional two-layer hydraulic model was established to describe the mechanism of transient cuttings transport with foam fluid in horizontal well section. The model was numerically calculated based on the modified SIMPLE algorithm, and the height of cuttings bed was predicted by the trial-and-error method. Sensitivity analysis was conducted on the affecting factors on the cuttings transport performance. Results show that cuttings deposition moves along the horizontal wellbore from the drilling bit, and finally achieves a steady state with dynamic balance. Dimensionless cuttings bed height decreases with the increase of foam quality or foam flow rate, but increases with the increase of drillpipe eccentricity, cuttings size or drilling rate. The influx of water and gas from the reservoir is helpful to improve the cuttings transport efficiency with foam. The proposed model offers theoretical guidelines for hydraulic parameter design and hole cleaning control in foamed horizontal drilling.展开更多
基金supported by the 111 Project from the Education Ministry of China(B07049)the National 11th Five-Year Plan Key Project(2006BAD08A05)Toxicity Variation of Wheat Stripe Rust Pathogen and Demonstration of Integrated Management of Stripe Rust, China (200903035-02)
文摘Stripe rust, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most widespread and destructive wheat diseases in many wheat-growing regions of the world. The winter wheat translocation line H9014-14-4-6-1 has all stage resistance. To identify stripe rust resistance genes, the segregating populations were developed from the cross between H9014-14-4-6-1 and Mingxian 169 （a wheat cultivar susceptible to all Pst races identified in China）. The seedlings of the parents and F1 plants, Fz, F3 and BC1 generations were tested with Pst races under controlled greenhouse conditions. Two genes for resistance to stripe rust were identified, one dominant gene conferred resistance to SUN11-4, temporarily designated YrH9014 and the other recessive gene conferred resistance to CYR33. The bulked segregant analysis and simple sequence repeat （SSR） markers were used to identify polymorphic markers associated with YrH9014. Seven polymorphic SSR markers were used to genotype the F2 population inoculated with SUN11-4. A linkage map was constructed according to the genotypes of seven SSR markers and resistance gene. The molecular map spanned 24.3 cM, and the genetic distance of the two closest markers Xbarc13 and Xbarc55 to gene locus was 1.4 and 3.6 cM, respectively. Based on the position of SSR marker, the resistance gene YrH9014 was located on chromosome arm 2BS. Amplification of a set of nulli-tetrasomic Chinese Spring lines with SSR marker Xbarc13 indicated that YrH9014 was located on chromosome 2B. Based on chromosomal location, the reaction patterns and pedigree analysis, YrH9014 should be a novel resistance gene to stripe rust. This new gene and flanking markers got from this study should be useful for marker-assisted selection （MAS） in breeding programs for stripe rust.
基金supported by the National High Technology Research and Development Program of China (863 Program, Grant No. 2006AA06Z218)the National Natural Science Foundation of China (Grant No. 50574062)
文摘With considering the interlayer mass transfer and fluid influx from the reservoir, a one-dimensional two-layer hydraulic model was established to describe the mechanism of transient cuttings transport with foam fluid in horizontal well section. The model was numerically calculated based on the modified SIMPLE algorithm, and the height of cuttings bed was predicted by the trial-and-error method. Sensitivity analysis was conducted on the affecting factors on the cuttings transport performance. Results show that cuttings deposition moves along the horizontal wellbore from the drilling bit, and finally achieves a steady state with dynamic balance. Dimensionless cuttings bed height decreases with the increase of foam quality or foam flow rate, but increases with the increase of drillpipe eccentricity, cuttings size or drilling rate. The influx of water and gas from the reservoir is helpful to improve the cuttings transport efficiency with foam. The proposed model offers theoretical guidelines for hydraulic parameter design and hole cleaning control in foamed horizontal drilling.
