目的探讨2型糖尿病(T2DM)、糖尿病肾病(DKD)患者血清总胆汁酸(TBA)水平的变化和影响TBA水平的相关因素。方法选取2017年1月-2018年10月在仁济医院住院的老年T2DM的445例患者作为DM组,同期非2型糖尿病患者作为对照组(NDM组,n=449)。进一...目的探讨2型糖尿病(T2DM)、糖尿病肾病(DKD)患者血清总胆汁酸(TBA)水平的变化和影响TBA水平的相关因素。方法选取2017年1月-2018年10月在仁济医院住院的老年T2DM的445例患者作为DM组,同期非2型糖尿病患者作为对照组(NDM组,n=449)。进一步将DM组分成非糖尿病肾组(NDKD组,n=280)与糖尿病肾病组(DKD组,n=165)组。收集研究对象的年龄、性别、身高、体重等一般资料、TBA和其他生化指标,及患者腰围(WC)、糖尿病病程(DD),分析影响TBA的相关因素和各组间临床资料差异性,并分析T2DM合并DKD的危险因素。结果在总人群中,血清TBA水平与年龄、胰岛素抵抗指数(HOMA-IR)、糖化血红蛋白(HbA1c)、糖化白蛋白(GA)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、三酰甘油(TG)呈正相关,与高密度脂蛋白胆固醇(HDL-C)呈负相关(均P<0.05)。在校正其他影响因素后,DM组血清TBA水平明显高于NDM组,3.90(95%CI:3.68~4.13)mol/L vs 2.91(95%CI:2.68~3.13)mol/L,P<0.05;DKD组比NDKD组患者血清TBA水平降低,3.10(95%CI:2.71~3.49)vs 4.39(95%CI:4.09~4.69)mol/L,P<0.05。在T2DM组中,以是否合并DKD为因变量,进行多因素Logistic回归分析,结果显示TBA是DKD的保护因素(OR=0.807,95%CI:0.728~0.894,P<0.05)。结论血清TBA水平与胰岛素抵抗水平呈正相关。T2DM患者血清TBA水平升高,但T2DM患者合并DKD时,血清TBA水平降低,胆汁酸(BA)可能是糖尿病肾病的保护因素。展开更多
目的探讨基质金属蛋白酶3(MMP3)对心肌纤维化细胞活力的影响。方法利用MMP3过表达载体及siRNA-MMP3转染大鼠心肌细胞H9C2,并用荧光定量PCR(RT-qPCR)检测转染效率。用不同浓度的血管紧张素II(AngII)处理H9C2细胞不同时间,通过RT-qPCR检...目的探讨基质金属蛋白酶3(MMP3)对心肌纤维化细胞活力的影响。方法利用MMP3过表达载体及siRNA-MMP3转染大鼠心肌细胞H9C2,并用荧光定量PCR(RT-qPCR)检测转染效率。用不同浓度的血管紧张素II(AngII)处理H9C2细胞不同时间,通过RT-qPCR检测细胞中Axl基因的表达情况。最后用CCK-8试剂盒检测MMP3过表达和敲低对心肌纤维化细胞活力的影响。结果RT-qPCR结果表明MMP3过表达和敲低的H9C2细胞成功构建,并且10-8 M AngII处理H9C2细胞24 h后,Axl的mRNA表达水平与未处理的相比降低(P<0.01)。CCK-8检测结果表明,与正常心肌细胞相比,心肌纤维化细胞的活力下降(P<0.05)。当心肌纤维化细胞中MMP3敲低后,其细胞活性进一步降低(P<0.05);当用100 M左卡尼汀处理后,其活力的变化趋势与敲低后的相似。而当MMP3过表达时,其细胞活性与用左卡尼汀处理正常细胞的相比增加(P<0.05)。结论MMP3敲低可能通过降低心肌纤维化细胞的活力,抑制细胞增殖,从而可能改善心肌纤维化,延缓心肌重构。展开更多
Aiming at the ″Made in China 2025″ strategy proposed by the state and combining with the popular the Internet, Internet of things, big data, machine learning and other technologies, Hadoop-based open source framewor...Aiming at the ″Made in China 2025″ strategy proposed by the state and combining with the popular the Internet, Internet of things, big data, machine learning and other technologies, Hadoop-based open source framework is adopted to realize the equipment data resource management platform of paper-making mill equipment. In this paper, the main function module of the platform is introduced, and some of operational effect map from the C paper-making mill are shown, and the key technologies in the platform construction is studied. It proposal to combine the internet, the internet of things, big data and machine learning to improve the informatization of the paper-making mill equipment, and ensure production safety, optimize technological process, improve product competitiveness, and also provide the theoretical guidance and practical basis for constructing the similar projects.展开更多
OBJECTIVE: To determine whether algal oligosaccharide affects the levels of parathyroid hormone 1-84(PTH1-84) and vascular endothelial growth factor(VEGF).METHODS: An osteoporosis rat model was established via bilater...OBJECTIVE: To determine whether algal oligosaccharide affects the levels of parathyroid hormone 1-84(PTH1-84) and vascular endothelial growth factor(VEGF).METHODS: An osteoporosis rat model was established via bilateral ovariectomy. The model rats were fed algal oligosaccharides(molecular weights:600-1, 200 Da) for 4 months. Bone mineral density(BMD) was then measured. MG-63 human osteoblastic cells were treated with algal oligosaccharides. The expression of PTH1-84 and VEGF was then examined. Oligosaccharide-treated cells were transfected with PTH1-84 short hairpin RNA(sh RNA), VEGF sh RNA, and PTH1-84-VEGF small interfering RNA(si RNA). The growth rates were then compared between transfected and non-transfected cells.RESULTS: Algal oligosaccharides increased the BMD of the osteoporosis rat model compared with untreated controls(P < 0.05). When MG-63 cells were treated with algal oligosaccharides, the growth rate increased by 25% compared with the control group at day 3(P < 0.05). In addition, the expression of PTH84 and VEGF was enhanced. Con-versely, when the cells were transfected with PTH84 sh RNA, VEGF sh RNA, or PTH1-84-VEGF si RNA, the growth rate was decreased by 17%, 35% and 70%, respectively, compared with controls at day 3(P < 0.05).CONCLUSION: Algal oligosaccharides ameliorate osteoporosis via up-regulation of PTH1-84 and VEGF. Algal oligosaccharides should be developed as a potential drug for osteoporosis treatment.展开更多
文摘目的探讨2型糖尿病(T2DM)、糖尿病肾病(DKD)患者血清总胆汁酸(TBA)水平的变化和影响TBA水平的相关因素。方法选取2017年1月-2018年10月在仁济医院住院的老年T2DM的445例患者作为DM组,同期非2型糖尿病患者作为对照组(NDM组,n=449)。进一步将DM组分成非糖尿病肾组(NDKD组,n=280)与糖尿病肾病组(DKD组,n=165)组。收集研究对象的年龄、性别、身高、体重等一般资料、TBA和其他生化指标,及患者腰围(WC)、糖尿病病程(DD),分析影响TBA的相关因素和各组间临床资料差异性,并分析T2DM合并DKD的危险因素。结果在总人群中,血清TBA水平与年龄、胰岛素抵抗指数(HOMA-IR)、糖化血红蛋白(HbA1c)、糖化白蛋白(GA)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、三酰甘油(TG)呈正相关,与高密度脂蛋白胆固醇(HDL-C)呈负相关(均P<0.05)。在校正其他影响因素后,DM组血清TBA水平明显高于NDM组,3.90(95%CI:3.68~4.13)mol/L vs 2.91(95%CI:2.68~3.13)mol/L,P<0.05;DKD组比NDKD组患者血清TBA水平降低,3.10(95%CI:2.71~3.49)vs 4.39(95%CI:4.09~4.69)mol/L,P<0.05。在T2DM组中,以是否合并DKD为因变量,进行多因素Logistic回归分析,结果显示TBA是DKD的保护因素(OR=0.807,95%CI:0.728~0.894,P<0.05)。结论血清TBA水平与胰岛素抵抗水平呈正相关。T2DM患者血清TBA水平升高,但T2DM患者合并DKD时,血清TBA水平降低,胆汁酸(BA)可能是糖尿病肾病的保护因素。
文摘目的探讨基质金属蛋白酶3(MMP3)对心肌纤维化细胞活力的影响。方法利用MMP3过表达载体及siRNA-MMP3转染大鼠心肌细胞H9C2,并用荧光定量PCR(RT-qPCR)检测转染效率。用不同浓度的血管紧张素II(AngII)处理H9C2细胞不同时间,通过RT-qPCR检测细胞中Axl基因的表达情况。最后用CCK-8试剂盒检测MMP3过表达和敲低对心肌纤维化细胞活力的影响。结果RT-qPCR结果表明MMP3过表达和敲低的H9C2细胞成功构建,并且10-8 M AngII处理H9C2细胞24 h后,Axl的mRNA表达水平与未处理的相比降低(P<0.01)。CCK-8检测结果表明,与正常心肌细胞相比,心肌纤维化细胞的活力下降(P<0.05)。当心肌纤维化细胞中MMP3敲低后,其细胞活性进一步降低(P<0.05);当用100 M左卡尼汀处理后,其活力的变化趋势与敲低后的相似。而当MMP3过表达时,其细胞活性与用左卡尼汀处理正常细胞的相比增加(P<0.05)。结论MMP3敲低可能通过降低心肌纤维化细胞的活力,抑制细胞增殖,从而可能改善心肌纤维化,延缓心肌重构。
文摘Aiming at the ″Made in China 2025″ strategy proposed by the state and combining with the popular the Internet, Internet of things, big data, machine learning and other technologies, Hadoop-based open source framework is adopted to realize the equipment data resource management platform of paper-making mill equipment. In this paper, the main function module of the platform is introduced, and some of operational effect map from the C paper-making mill are shown, and the key technologies in the platform construction is studied. It proposal to combine the internet, the internet of things, big data and machine learning to improve the informatization of the paper-making mill equipment, and ensure production safety, optimize technological process, improve product competitiveness, and also provide the theoretical guidance and practical basis for constructing the similar projects.
文摘OBJECTIVE: To determine whether algal oligosaccharide affects the levels of parathyroid hormone 1-84(PTH1-84) and vascular endothelial growth factor(VEGF).METHODS: An osteoporosis rat model was established via bilateral ovariectomy. The model rats were fed algal oligosaccharides(molecular weights:600-1, 200 Da) for 4 months. Bone mineral density(BMD) was then measured. MG-63 human osteoblastic cells were treated with algal oligosaccharides. The expression of PTH1-84 and VEGF was then examined. Oligosaccharide-treated cells were transfected with PTH1-84 short hairpin RNA(sh RNA), VEGF sh RNA, and PTH1-84-VEGF small interfering RNA(si RNA). The growth rates were then compared between transfected and non-transfected cells.RESULTS: Algal oligosaccharides increased the BMD of the osteoporosis rat model compared with untreated controls(P < 0.05). When MG-63 cells were treated with algal oligosaccharides, the growth rate increased by 25% compared with the control group at day 3(P < 0.05). In addition, the expression of PTH84 and VEGF was enhanced. Con-versely, when the cells were transfected with PTH84 sh RNA, VEGF sh RNA, or PTH1-84-VEGF si RNA, the growth rate was decreased by 17%, 35% and 70%, respectively, compared with controls at day 3(P < 0.05).CONCLUSION: Algal oligosaccharides ameliorate osteoporosis via up-regulation of PTH1-84 and VEGF. Algal oligosaccharides should be developed as a potential drug for osteoporosis treatment.
