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Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics 被引量:3
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作者 YIN jing Feng wang jin ling +8 位作者 TANG Qing DING Yu Lin TAO XiaoYan LI Hao SONG Miao GUO ZhenYang SHEN Xin Xin LIANG Guo Dong wang Feng Long 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第1期35-44,共10页
Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2012, and analyze their eti... Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2012, and analyze their etiological characteristics. Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens. Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS, and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate. Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2012, which led to a different etiologic characteristics of the epidemic situation. 展开更多
关键词 RABIES COW HISTOPATHOLOGY PHYLOGENY
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