花生是重要的油料作物之一,其籽仁品质直接影响加工特性,是花生品质评价的重要指标。建立花生籽仁品质高通量分析模型,快速高效地对花生籽仁品质进行评价,可显著提高花生育种效率。本研究选用宇花14与LOP215杂交构建的140个RIL家系和35...花生是重要的油料作物之一,其籽仁品质直接影响加工特性,是花生品质评价的重要指标。建立花生籽仁品质高通量分析模型,快速高效地对花生籽仁品质进行评价,可显著提高花生育种效率。本研究选用宇花14与LOP215杂交构建的140个RIL家系和35份其他品系为建模材料,使用Thermo公司生产的AntarisⅡ型傅立叶变换近红外光谱分析仪对175份样品籽仁进行光谱采集。采用索氏提取法测定籽仁含油量,杜马斯定氮法测定蛋白含量,蒽酮比色法测定糖含量,气相色谱法测定脂肪酸含量。利用偏最小二乘法(partial least squares,PLS)构建花生籽仁含油量、蛋白含量、糖含量以及部分脂肪酸含量的多粒近红外定标模型。选用未参与建模的30份花生样品对该模型进行验证,模型决定系数R~2值均大于0.9000,表明该模型可用于花生籽仁品质性状的分析预测。本研究为花生籽仁品质性状高通量表型分析提供了检测模型。展开更多
花生(Arachis hypogaea L.)籽仁含油量是花生品质评价的重要指标,建立快速高效的含油量检测方法,对加快高油花生品种选育意义重大。本研究选用高油亲本宇花14(含油量59.32%)与低油亲本LOP215(含油量48.97%)杂交构建的RIL群体为建模材料...花生(Arachis hypogaea L.)籽仁含油量是花生品质评价的重要指标,建立快速高效的含油量检测方法,对加快高油花生品种选育意义重大。本研究选用高油亲本宇花14(含油量59.32%)与低油亲本LOP215(含油量48.97%)杂交构建的RIL群体为建模材料,使用Thermo公司(美国)生产的AntarisⅡ型傅立叶变换近红外光谱分析仪对229份样品籽仁进行光谱采集,随后测定籽仁含油量。利用偏最小二乘法(partial least squares,PLS)构建花生籽仁含油量近红外定标模型,该模型的内部验证均方差(root mean square error of cross validation,RMSECV)为0.885,相关系数R^(2)=0.9147。选用未参与建模的21份花生材料对该模型进行外部验证,模型预测值和化学测定值的决定系数R^(2)=0.9492,表明该模型可适用于花生籽仁含油量检测。利用该模型对宇花14与LOP215杂交后代群体进行筛选,获得含油量超过55%的优良株系21个,含油量低于48%的株系9个,可为花生高低含油量品种选育提供种质材料。展开更多
Peanut (Arachis hypogaea L.) is an important oil crop globally and high oil content is one of the major targets in peanut breeding programs. Previous studies indicated that the osmotic pressure (OP) of the leaves of p...Peanut (Arachis hypogaea L.) is an important oil crop globally and high oil content is one of the major targets in peanut breeding programs. Previous studies indicated that the osmotic pressure (OP) of the leaves of peanut plants subjected to drought stress was negatively correlated with kernel oil content. Based on this knowledge, we established a practical and reliable method for creating new peanut varieties with high oil content using in vitro mutagenesis and directional OP-based selection. Using embryonic leaflets of peanut variety Huayu 20 as explants, pingyangmycin (PYM) as the mutagen, and hydroxyproline (HYP) as the OP regulator, we developed 15 HYP-tolerant regenerated plants. For each regenerated plant, we selected offspring with oil content>55% (relative to 49.5% for Huayu 20). We developed and released three new peanut varieties with high yield and high oil content from the offspring of the HYP-tolerant regenerated plants. The three new varieties were named as Yuhua 4, Yuhua 9 and Yuhua 14 and their oil contents were 57.7, 61.1 and 59.3%, respectively. The results indicate that in vitro mutagenesis with PYM followed by directed screening with HYP is a useful approach for breeding peanut varieties with high oil contents.展开更多
文摘花生是重要的油料作物之一,其籽仁品质直接影响加工特性,是花生品质评价的重要指标。建立花生籽仁品质高通量分析模型,快速高效地对花生籽仁品质进行评价,可显著提高花生育种效率。本研究选用宇花14与LOP215杂交构建的140个RIL家系和35份其他品系为建模材料,使用Thermo公司生产的AntarisⅡ型傅立叶变换近红外光谱分析仪对175份样品籽仁进行光谱采集。采用索氏提取法测定籽仁含油量,杜马斯定氮法测定蛋白含量,蒽酮比色法测定糖含量,气相色谱法测定脂肪酸含量。利用偏最小二乘法(partial least squares,PLS)构建花生籽仁含油量、蛋白含量、糖含量以及部分脂肪酸含量的多粒近红外定标模型。选用未参与建模的30份花生样品对该模型进行验证,模型决定系数R~2值均大于0.9000,表明该模型可用于花生籽仁品质性状的分析预测。本研究为花生籽仁品质性状高通量表型分析提供了检测模型。
文摘花生(Arachis hypogaea L.)籽仁含油量是花生品质评价的重要指标,建立快速高效的含油量检测方法,对加快高油花生品种选育意义重大。本研究选用高油亲本宇花14(含油量59.32%)与低油亲本LOP215(含油量48.97%)杂交构建的RIL群体为建模材料,使用Thermo公司(美国)生产的AntarisⅡ型傅立叶变换近红外光谱分析仪对229份样品籽仁进行光谱采集,随后测定籽仁含油量。利用偏最小二乘法(partial least squares,PLS)构建花生籽仁含油量近红外定标模型,该模型的内部验证均方差(root mean square error of cross validation,RMSECV)为0.885,相关系数R^(2)=0.9147。选用未参与建模的21份花生材料对该模型进行外部验证,模型预测值和化学测定值的决定系数R^(2)=0.9492,表明该模型可适用于花生籽仁含油量检测。利用该模型对宇花14与LOP215杂交后代群体进行筛选,获得含油量超过55%的优良株系21个,含油量低于48%的株系9个,可为花生高低含油量品种选育提供种质材料。
基金This research was funded by the National Natural Science Foundation of China(31571705 and 31872875)the Key R&D Program of Shandong Province,China(2018GNC111014)the Open Oroject of the Key Lab of Oil Crop Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs of China(KF2018008).
文摘Peanut (Arachis hypogaea L.) is an important oil crop globally and high oil content is one of the major targets in peanut breeding programs. Previous studies indicated that the osmotic pressure (OP) of the leaves of peanut plants subjected to drought stress was negatively correlated with kernel oil content. Based on this knowledge, we established a practical and reliable method for creating new peanut varieties with high oil content using in vitro mutagenesis and directional OP-based selection. Using embryonic leaflets of peanut variety Huayu 20 as explants, pingyangmycin (PYM) as the mutagen, and hydroxyproline (HYP) as the OP regulator, we developed 15 HYP-tolerant regenerated plants. For each regenerated plant, we selected offspring with oil content>55% (relative to 49.5% for Huayu 20). We developed and released three new peanut varieties with high yield and high oil content from the offspring of the HYP-tolerant regenerated plants. The three new varieties were named as Yuhua 4, Yuhua 9 and Yuhua 14 and their oil contents were 57.7, 61.1 and 59.3%, respectively. The results indicate that in vitro mutagenesis with PYM followed by directed screening with HYP is a useful approach for breeding peanut varieties with high oil contents.
