This paper concerns the Cauchy problem for compressible Navier-Stokes equations.The weak dissipative structure is explored and a new proof for the classical solutions are shown to exist globally in time if the initial...This paper concerns the Cauchy problem for compressible Navier-Stokes equations.The weak dissipative structure is explored and a new proof for the classical solutions are shown to exist globally in time if the initial data is sufficiently small.展开更多
目的探讨血清中抗-PTCH1自身抗体对甲胎蛋白(AFP)阴性肝癌的诊断价值。方法在2019年3月—2022年9月应用间接酶联免疫吸附实验(ELISA)检测来自于郑州(176例)、南昌(49例)和北京(38例)3个临床中心共计263例AFP阴性肝癌和对照血清中抗-PTCH...目的探讨血清中抗-PTCH1自身抗体对甲胎蛋白(AFP)阴性肝癌的诊断价值。方法在2019年3月—2022年9月应用间接酶联免疫吸附实验(ELISA)检测来自于郑州(176例)、南昌(49例)和北京(38例)3个临床中心共计263例AFP阴性肝癌和对照血清中抗-PTCH1自身抗体水平;非参数检验(Mann-Whitney U test)比较病例组和对照组血清抗-PTCH1自身抗体水平的差异;绘制ROC曲线,计算ROC曲线下面积(AUC)、灵敏度、特异度等指标评价其诊断AFP阴性肝癌的价值;探讨抗-PTCH1自身抗体与临床生化指标联合应用于AFP阴性肝癌的诊断效果。结果3个临床中心的AFP阴性肝癌患者组血清抗-PTCH1自身抗体水平均高于对照组(P<0.05);抗-PTCH1自身抗体在郑州、南昌和北京诊断AFP阴性肝癌的ROC曲线下面积分别为0.692、0.763和0.845,灵敏度分别为44.3%、50.0%和40.0%,特异度分别为81.0%、88.0%和100.0%;联合临床指标AST和ALT构建诊断模型后在郑州、南昌和北京诊断AFP阴性肝癌的ROC曲线下面积分别为0.844、0.843和0.873。结论抗-PTCH1自身抗体对临床AFP阴性肝癌患者有一定诊断价值,联合临床指标ALT和AST的诊断AFP阴性肝癌效果更好。展开更多
Objective:To investigate the regulatory roles of Shexiang Baoxin Pill(SXBXW)in neointimal formation and vascular smooth muscle cells(VSMCs)invasion and apoptosis as well as the potential molecular mechanisms using cul...Objective:To investigate the regulatory roles of Shexiang Baoxin Pill(SXBXW)in neointimal formation and vascular smooth muscle cells(VSMCs)invasion and apoptosis as well as the potential molecular mechanisms using cultured VSMCs model of vascular injury(platelet-derived growth factor(PDGF)-BBstimulated)in vitro.Methods:VSMCs were randomly assigned to 5 groups:blank,PDGF-BB(20 ng/mL+0.1%DMSO),SXBXW-L(PDGF-BB 20 ng/mL+SXBXW low dose 0.625 g/L),SXBXW-M(PDGF-BB 20 ng/mL+SXBXW medium dose 1.25 g/L)and SXBXW-H(PDGF-BB 20 ng/mL+SXBXW high dose 2.5 g/L)group.Cell proliferation was assessed using cell counting kit-8(CCK-8)assay and bromodeoxyuridine(BrdU)incorporation assay,the migration effects were detected by Transwell assay,cell apoptosis rate was measured by the Annexin V/propidium iodide(PI)apoptosis kit.The markers of contractile phenotype of VSMCs were detected with immunofluorescent staining.To validate the effects of miR-451 in regulating proliferation,migration and apoptosis treated with SXBXW,miR-451 overexpression experiments were performed,the VSMCs were exposed to PDGF-BB 20 ng/mL+0.1%DMSO and later divided into 4 groups:mimic-NC(multiplicity of infection,MOI=50),SXBXW(1.25 g/L)+mimic-NC,mimic-miR451(MOI=50),and SXBXW(1.25 g/L)+mimic-miR451,and alterations of proteins related to the miR-451 pathway were analyzed using Western blot.Results:PDGF-BB induced VSMCs injury causes acceleration of proliferation and migration.SXBXW inhibited phenotypic switching,proliferation and migration and promoted cell apoptosis in PDGF-BB-induced VSMCs.In addition,miR-451 was shown to be down-regulated in the VSMCs following PDGF-BB stimulation.SXBXW treatment enhanced the expression of miR-451 in PDGF-BB-induced VSMCs(P<0.05).Compared with SXBXW+mimic-NC and mimicmiR451 groups,the expression of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta(Ywhaz)and p53 was further reduced in SXBXW+mimic-miR451 group,while activating transcription factor 2(ATF2)was increased in VSMCs(P<0.05).Conclusion:SXBXW regulated proliferation,migration and apoptosis via activation of miR-451 through ATF2,p53 and Ywhaz in PDGF-BB-stimulated VSMCs.展开更多
基金Project(51971106) supported by the National Natural Science Foundation of ChinaProject(GJJ191094) supported by the Education Department of Jiangxi Province,China+2 种基金Project(PA2019GDZC0096) supported by Fundamental Research Funds for the Central Universities of ChinaProject(2019-MS-171) supported by the Liaoning Natural Science Foundation,ChinaProject supported by Programs for Liaoning Innovative Talents/Groups and Liaoning Distinguished Professors,China。
基金The Young Excellent Teacher Program Foundation of Shanghai
文摘This paper concerns the Cauchy problem for compressible Navier-Stokes equations.The weak dissipative structure is explored and a new proof for the classical solutions are shown to exist globally in time if the initial data is sufficiently small.
文摘目的探讨血清中抗-PTCH1自身抗体对甲胎蛋白(AFP)阴性肝癌的诊断价值。方法在2019年3月—2022年9月应用间接酶联免疫吸附实验(ELISA)检测来自于郑州(176例)、南昌(49例)和北京(38例)3个临床中心共计263例AFP阴性肝癌和对照血清中抗-PTCH1自身抗体水平;非参数检验(Mann-Whitney U test)比较病例组和对照组血清抗-PTCH1自身抗体水平的差异;绘制ROC曲线,计算ROC曲线下面积(AUC)、灵敏度、特异度等指标评价其诊断AFP阴性肝癌的价值;探讨抗-PTCH1自身抗体与临床生化指标联合应用于AFP阴性肝癌的诊断效果。结果3个临床中心的AFP阴性肝癌患者组血清抗-PTCH1自身抗体水平均高于对照组(P<0.05);抗-PTCH1自身抗体在郑州、南昌和北京诊断AFP阴性肝癌的ROC曲线下面积分别为0.692、0.763和0.845,灵敏度分别为44.3%、50.0%和40.0%,特异度分别为81.0%、88.0%和100.0%;联合临床指标AST和ALT构建诊断模型后在郑州、南昌和北京诊断AFP阴性肝癌的ROC曲线下面积分别为0.844、0.843和0.873。结论抗-PTCH1自身抗体对临床AFP阴性肝癌患者有一定诊断价值,联合临床指标ALT和AST的诊断AFP阴性肝癌效果更好。
基金Supported by Shanghai Key Laboratory of Traditional Chinese Clinical Medicine(No.14DZ2273200)Shanghai Municipal Key Clinical Specialty(Department of TCM Cardiology,No.shslczdzk05301)。
文摘Objective:To investigate the regulatory roles of Shexiang Baoxin Pill(SXBXW)in neointimal formation and vascular smooth muscle cells(VSMCs)invasion and apoptosis as well as the potential molecular mechanisms using cultured VSMCs model of vascular injury(platelet-derived growth factor(PDGF)-BBstimulated)in vitro.Methods:VSMCs were randomly assigned to 5 groups:blank,PDGF-BB(20 ng/mL+0.1%DMSO),SXBXW-L(PDGF-BB 20 ng/mL+SXBXW low dose 0.625 g/L),SXBXW-M(PDGF-BB 20 ng/mL+SXBXW medium dose 1.25 g/L)and SXBXW-H(PDGF-BB 20 ng/mL+SXBXW high dose 2.5 g/L)group.Cell proliferation was assessed using cell counting kit-8(CCK-8)assay and bromodeoxyuridine(BrdU)incorporation assay,the migration effects were detected by Transwell assay,cell apoptosis rate was measured by the Annexin V/propidium iodide(PI)apoptosis kit.The markers of contractile phenotype of VSMCs were detected with immunofluorescent staining.To validate the effects of miR-451 in regulating proliferation,migration and apoptosis treated with SXBXW,miR-451 overexpression experiments were performed,the VSMCs were exposed to PDGF-BB 20 ng/mL+0.1%DMSO and later divided into 4 groups:mimic-NC(multiplicity of infection,MOI=50),SXBXW(1.25 g/L)+mimic-NC,mimic-miR451(MOI=50),and SXBXW(1.25 g/L)+mimic-miR451,and alterations of proteins related to the miR-451 pathway were analyzed using Western blot.Results:PDGF-BB induced VSMCs injury causes acceleration of proliferation and migration.SXBXW inhibited phenotypic switching,proliferation and migration and promoted cell apoptosis in PDGF-BB-induced VSMCs.In addition,miR-451 was shown to be down-regulated in the VSMCs following PDGF-BB stimulation.SXBXW treatment enhanced the expression of miR-451 in PDGF-BB-induced VSMCs(P<0.05).Compared with SXBXW+mimic-NC and mimicmiR451 groups,the expression of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta(Ywhaz)and p53 was further reduced in SXBXW+mimic-miR451 group,while activating transcription factor 2(ATF2)was increased in VSMCs(P<0.05).Conclusion:SXBXW regulated proliferation,migration and apoptosis via activation of miR-451 through ATF2,p53 and Ywhaz in PDGF-BB-stimulated VSMCs.
