目的研究关节镜下后踝三入路法切除治疗成人疼痛性跟距骨桥的疗效,以期为临床该病患者的治疗提供方案。方法选择首都医科大学附属北京同仁医院从2021年2~12月收治的11例成人疼痛性跟距骨桥患者。所有受试者均开展经典后踝内外侧入路联...目的研究关节镜下后踝三入路法切除治疗成人疼痛性跟距骨桥的疗效,以期为临床该病患者的治疗提供方案。方法选择首都医科大学附属北京同仁医院从2021年2~12月收治的11例成人疼痛性跟距骨桥患者。所有受试者均开展经典后踝内外侧入路联合外侧高位入路切除术治疗。术前及术后随访,均行患侧踝关节负重正侧位X线片,CT检查。采用疼痛视觉模拟评分(visual analogue scale,VAS)及美国足踝外科协会(American Orthopaedic Foot and Ankle Society Ankle-Hindfoot Scale,AOFAS)踝-后足评分评价疼痛及足部功能改善情况。结果11例患者术后全部获得随访,随访时间6~14个月,平均11个月。术后切口均Ⅰ期愈合,无伤口感染、血管神经肌腱损伤、下肢深静脉血栓形成等并发症发生。术后踝关节疼痛及足部功能显著改善。末次随访VAS中位数1.0分,与术前VAS中位数6.0分比较,差异有统计学意义(P<0.05);末次随访AOFAS踝-后足评分(93.7±4.6)分,与术前(62.4±9.7)分比较,差异有统计学意义(P<0.001)。结论关节镜下后踝三入路可更好显露跟距骨桥,视野更广阔,空间感更好,操作过程更灵活,切除时不易伤及血管神经,有利于获得更好疗效。展开更多
MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fr...MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions.展开更多
In eukaryotes, alternative splicing(AS) is one of the posttranscriptional regulatory mechanisms that play important roles by generating transcriptome diversity. To obtain a global view of AS and its dynamics during to...In eukaryotes, alternative splicing(AS) is one of the posttranscriptional regulatory mechanisms that play important roles by generating transcriptome diversity. To obtain a global view of AS and its dynamics during tomato fruit development, we analyzed the AS events using a large amount of transcriptome datasets. Same with other plant species, about half of the expressed multiexonic genes were alternatively spliced in tomato. Besides that, our further analyzation of RNA-seq datasets of ovule and pericarp at early fruit developmental stages reveals that the dynamic alteration of AS events occurred in specific tissues and AS was regulated spatially and temporally during early fruit development in tomato. By investigating the sequence variations at splice sites causing differential AS events between tomato cultivar ‘Moneymaker' and wild species Solanum pimpinellifolium PI365967, we uncover that AS may play the regulatory roles during domestication of tomato. Taken together, our results provided the global AS pattern in tomato and highlighted the importance of AS during tomato fruit development and domestication.展开更多
文摘目的研究关节镜下后踝三入路法切除治疗成人疼痛性跟距骨桥的疗效,以期为临床该病患者的治疗提供方案。方法选择首都医科大学附属北京同仁医院从2021年2~12月收治的11例成人疼痛性跟距骨桥患者。所有受试者均开展经典后踝内外侧入路联合外侧高位入路切除术治疗。术前及术后随访,均行患侧踝关节负重正侧位X线片,CT检查。采用疼痛视觉模拟评分(visual analogue scale,VAS)及美国足踝外科协会(American Orthopaedic Foot and Ankle Society Ankle-Hindfoot Scale,AOFAS)踝-后足评分评价疼痛及足部功能改善情况。结果11例患者术后全部获得随访,随访时间6~14个月,平均11个月。术后切口均Ⅰ期愈合,无伤口感染、血管神经肌腱损伤、下肢深静脉血栓形成等并发症发生。术后踝关节疼痛及足部功能显著改善。末次随访VAS中位数1.0分,与术前VAS中位数6.0分比较,差异有统计学意义(P<0.05);末次随访AOFAS踝-后足评分(93.7±4.6)分,与术前(62.4±9.7)分比较,差异有统计学意义(P<0.001)。结论关节镜下后踝三入路可更好显露跟距骨桥,视野更广阔,空间感更好,操作过程更灵活,切除时不易伤及血管神经,有利于获得更好疗效。
基金supported by the National Natural Science Foundation of China (31471874)the National Key Research and Development Program of China (2016YFD0101007 and 2016YFD0101703)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
文摘MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions.
基金supported by the National Natural Science Foundation of China (Grant No. 31572140 and No. 31570674)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
文摘In eukaryotes, alternative splicing(AS) is one of the posttranscriptional regulatory mechanisms that play important roles by generating transcriptome diversity. To obtain a global view of AS and its dynamics during tomato fruit development, we analyzed the AS events using a large amount of transcriptome datasets. Same with other plant species, about half of the expressed multiexonic genes were alternatively spliced in tomato. Besides that, our further analyzation of RNA-seq datasets of ovule and pericarp at early fruit developmental stages reveals that the dynamic alteration of AS events occurred in specific tissues and AS was regulated spatially and temporally during early fruit development in tomato. By investigating the sequence variations at splice sites causing differential AS events between tomato cultivar ‘Moneymaker' and wild species Solanum pimpinellifolium PI365967, we uncover that AS may play the regulatory roles during domestication of tomato. Taken together, our results provided the global AS pattern in tomato and highlighted the importance of AS during tomato fruit development and domestication.
