As the international landscape evolves at a faster pace in recent years,the strategic autonomy of the Middle Eastern countries has strengthened.Giving priority to development has become a common choice of countries in...As the international landscape evolves at a faster pace in recent years,the strategic autonomy of the Middle Eastern countries has strengthened.Giving priority to development has become a common choice of countries in the region.There’s a stronger“wave of reconciliation”.The Middle Eastern countries has gained a new position in the international system,becoming an important force in the Global South.展开更多
Middle East security has become a prominent global issue which is constantly exacerbating the uncertainty of the region and the world. Establishing a stable and valid security governance system is imperative for the r...Middle East security has become a prominent global issue which is constantly exacerbating the uncertainty of the region and the world. Establishing a stable and valid security governance system is imperative for the region, but is bound to be a long and arduous process through internal and external coordination and appropriate strategies.展开更多
Hegemonism and power politics are the greatest threats to global security, development and governance. US foreign policy has given rise to serious global security crises and security dilemmas. The Russia-Ukraine confl...Hegemonism and power politics are the greatest threats to global security, development and governance. US foreign policy has given rise to serious global security crises and security dilemmas. The Russia-Ukraine conflict is a typical case of the current predicament of global security governance. The conflict has exacerbated multiple global security crises, which raises the urgency to promote the construction of a global security governance system.展开更多
Located at the carboxyl terminal of the human papillomavirus major capsid protein L1, helix-5（h5） is cru-cial to L1 folding and pentamer formation. Site-directed mutagenesis of the leucine residue on site 469 into l...Located at the carboxyl terminal of the human papillomavirus major capsid protein L1, helix-5（h5） is cru-cial to L1 folding and pentamer formation. Site-directed mutagenesis of the leucine residue on site 469 into lysine, alanine, serine and glycine was performed to explore the effect of the resultant mutations on L 1 pentamer formation. The soluble yields of the L1 pentamers of the L469A and L469K mutants were nearly two fold higher than that of the wild type. Molecular dynamics simulation was then performed to reveal the intrinsic mechanisms involved in the improvement of L 1 pentamer yield. Accordingly, the secondary structures of h5, β-G2, β-B1, β-C, β-D, and β-F were altered. The altered structures improved the hydrophobic interaction between h5 and fl-core ＂jelly＂ and the stability of h5. The hydrophobic surface area of residue 469 was reduced by 50% relative to that of the wild type. The C--O group of residue 469 and C--N group of L470 were both exposed to the solvent in the L469A mutant. These modifications may account for the increased solubility and stability and the promotion of pentamer formation induced by the point mutation. Therefore, the changes in the hydrophobic properties of h5 and the core structure determined the pentamer formation and solubility. This study may assist the development of a cost-effective platform for the production of prophylactic virus-like particle vaccines.展开更多
文摘As the international landscape evolves at a faster pace in recent years,the strategic autonomy of the Middle Eastern countries has strengthened.Giving priority to development has become a common choice of countries in the region.There’s a stronger“wave of reconciliation”.The Middle Eastern countries has gained a new position in the international system,becoming an important force in the Global South.
文摘Middle East security has become a prominent global issue which is constantly exacerbating the uncertainty of the region and the world. Establishing a stable and valid security governance system is imperative for the region, but is bound to be a long and arduous process through internal and external coordination and appropriate strategies.
文摘Hegemonism and power politics are the greatest threats to global security, development and governance. US foreign policy has given rise to serious global security crises and security dilemmas. The Russia-Ukraine conflict is a typical case of the current predicament of global security governance. The conflict has exacerbated multiple global security crises, which raises the urgency to promote the construction of a global security governance system.
基金Supported by the National Natural Science Foundation of China（Nos. 91027027, 21373101 ）.
文摘Located at the carboxyl terminal of the human papillomavirus major capsid protein L1, helix-5（h5） is cru-cial to L1 folding and pentamer formation. Site-directed mutagenesis of the leucine residue on site 469 into lysine, alanine, serine and glycine was performed to explore the effect of the resultant mutations on L 1 pentamer formation. The soluble yields of the L1 pentamers of the L469A and L469K mutants were nearly two fold higher than that of the wild type. Molecular dynamics simulation was then performed to reveal the intrinsic mechanisms involved in the improvement of L 1 pentamer yield. Accordingly, the secondary structures of h5, β-G2, β-B1, β-C, β-D, and β-F were altered. The altered structures improved the hydrophobic interaction between h5 and fl-core ＂jelly＂ and the stability of h5. The hydrophobic surface area of residue 469 was reduced by 50% relative to that of the wild type. The C--O group of residue 469 and C--N group of L470 were both exposed to the solvent in the L469A mutant. These modifications may account for the increased solubility and stability and the promotion of pentamer formation induced by the point mutation. Therefore, the changes in the hydrophobic properties of h5 and the core structure determined the pentamer formation and solubility. This study may assist the development of a cost-effective platform for the production of prophylactic virus-like particle vaccines.
