特发性肺纤维化(idiopathic pulmonary fibrosis,IPF)是一种病因复杂且缺乏有效治疗手段的严重慢性肺部疾病。该研究通过构建转基因小鼠模型,特异性地在肺泡Ⅱ型上皮细胞中过表达肺表面活性蛋白B(surfactant protein B,SP-B),探讨其在IP...特发性肺纤维化(idiopathic pulmonary fibrosis,IPF)是一种病因复杂且缺乏有效治疗手段的严重慢性肺部疾病。该研究通过构建转基因小鼠模型,特异性地在肺泡Ⅱ型上皮细胞中过表达肺表面活性蛋白B(surfactant protein B,SP-B),探讨其在IPF中的功能及其作用机制。研究结果发现了SP-B的过表达会导致小鼠肺组织结构受损、细胞外基质异常沉积,并引发肺部炎症反应,揭示了SP-B在IPF病理进程中的重要作用。此外,SP-B可能通过调控TGF-β信号通路影响细胞增殖和细胞外基质蛋白表达,从而促进肺纤维化的发展。该研究为IPF的病因学研究提供了新的视角,并为基于Sftpb基因的治疗策略提供了潜在的靶点。展开更多
Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain l...Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis.展开更多
文摘特发性肺纤维化(idiopathic pulmonary fibrosis,IPF)是一种病因复杂且缺乏有效治疗手段的严重慢性肺部疾病。该研究通过构建转基因小鼠模型,特异性地在肺泡Ⅱ型上皮细胞中过表达肺表面活性蛋白B(surfactant protein B,SP-B),探讨其在IPF中的功能及其作用机制。研究结果发现了SP-B的过表达会导致小鼠肺组织结构受损、细胞外基质异常沉积,并引发肺部炎症反应,揭示了SP-B在IPF病理进程中的重要作用。此外,SP-B可能通过调控TGF-β信号通路影响细胞增殖和细胞外基质蛋白表达,从而促进肺纤维化的发展。该研究为IPF的病因学研究提供了新的视角,并为基于Sftpb基因的治疗策略提供了潜在的靶点。
基金supported by a grant from the PhD fellowship program of Soochow Universitythe National Natural Science Foundation of China(No.41276134)
文摘Volatile organic compounds in marine algae have been reported to comprise characteristic flavor of algae and play an important role in their growth, development and defensive response. Yet their biogeneration remain largely unknown. Here we studied the composition of volatile compouds in Pyropia yezoensis and their variations in response to methyl jasmonate(MeJA) and diethyldithiocarbamic acid(DIECA) treatment using gas chromatography-mass spectrometry(GC-MS). A total of 44 compounds belonging to the following chemical classes(n) were identified, including aldehydes(11), alcohols(8), acids and esters(6), alkanes(5), ketones(5), alkenes(3), and S-or N-containing miscellaneous compounds(6). External treatment with plant hormone MeJA increased the content of 1-dodecanol, 4-heptenal, and 2-propenoic acid-2-methyl dodecylester, but decreased the content of phytol, 3-heptadecene, 2-pentadecanone, and isophytol. When pretreated with DIECA, an inhibitor of the octadecanoid pathway leading to the biosynthesis of endogeneous jasmonates and some secondary metabolites, phytol and isophytol were increased, while 4-heptenal, 1-dodecanol, and 2-propenoic acid-2-methyl dodecylester were decreased, both of which were negatively correlated with their variations under MeJA treatment. Collectively, these results suggest that MeJA does affect the volatile composition of P. yezoensis, and the octadecanoid pathway together with endogenous jasmonate pathway may be involved in the biosynthesis of volatile compounds, thereby providing some preliminary envision on the composition and biogeneration of volatile compounds in P. yezoensis.
