MicroRNAs(miRNAs) are an abundant class of conserved, non-coding small RNAs that play important role in gene regulation at post translational level. There have been no reports on the miRNAs of the Antarctic krill E up...MicroRNAs(miRNAs) are an abundant class of conserved, non-coding small RNAs that play important role in gene regulation at post translational level. There have been no reports on the miRNAs of the Antarctic krill E uphausia superba despite the species' crucial position in Antarctic food webs. Two small RNA libraries were constructed from eyestalk and muscle, subsequently, and deep sequencing analysis was performed to investigate and profile E. superba miRNAs. A total of 19 304 586 and 23 005 104 unique sequences were obtained from the eyestalk and muscle, respectively. After compared the small RNA sequences with the Rfam database, 12 342 039 and 7 907 477 reads in eyestalk and muscle were matched to the transcriptome sequence of E. superba. A total of 236 distinct miRNAs were identified after annotation to known animal miRNAs registered in miRBase 21. In both libraries, the most abundant known miRNA were miR-750 with 92 583 reads in muscle and miR-1304-3 p with 56 386 reads in eyestalk while the average value was less than 106, revealing a wide range of different expression levels. In addition, miR-277 a enriched in both libraries and may be involved in modulating Krebs cycle by targeting to Vimar. Differential expression analysis showed that 56 mature miRNAs were significantly up/down regulated according to expression fold change. It appeared that the expression of several abundant miRNAs maybe tissue-specific or tissue-bias. Notably, the expression pattern of miR-750 and miR-1 family, which was suggested as the crucial candidates, involved in muscle development. Taken together, this study provides the first miRNA profile of E. superba and some of these miRNAs were expected to play important role in immune response, reproduction, energy metabolism, and muscle development and so on. Thus, the results provides a reference for functional studies of miRNAs in E. superba.展开更多
目的利用文献计量学方法分析国内外治疗药物监测领域热点和趋势,结合复旦大学附属中山医院厦门医院(本院)2022年开展的治疗药物监测情况进行回顾性分析,为促进临床合理用药提供参考。方法基于Web of Science核心数据库和中国知网(CNKI)...目的利用文献计量学方法分析国内外治疗药物监测领域热点和趋势,结合复旦大学附属中山医院厦门医院(本院)2022年开展的治疗药物监测情况进行回顾性分析,为促进临床合理用药提供参考。方法基于Web of Science核心数据库和中国知网(CNKI)使用CiteSpace软件对2019—2023年关于治疗药物监测领域的研究进行关键词的共现分析、聚类分析。结果筛选后纳入1301篇英文文献、330篇中文文献进行文献计量学分析。国内外研究中的高频关键词包括治疗药物监测的药物以及分析方法。2022年本院开展药物浓度检测总例数为864例。监测排名前三位的药物分别是他克莫司(37.27%)、雷帕霉素(18.17%)和万古霉素(14.35%)。他克莫司的治疗药物浓度结果处于有效浓度范围占比最高为90.06%,其次是雷帕霉素为85.35%,地高辛为85.00%。结论目前治疗药物监测研究主要为万古霉素、地高辛、抗癫痫药物的合理用药。本院搭建临床药师参与报告解读的治疗药物监测平台,还需关注抗精神类药物以及抗肿瘤药物的治疗药物监测。展开更多
开展水槽试验获得铜合金编织网网片的阻力,以分析铜合金编织网网衣的阻力变化规律及水动力性能。试验制作了10片0.25 m^(2)不同铜丝直径及不同网目尺寸的铜合金编织网网片,于拖车静水槽中采用三分力传感器连接网片以测试网片受力,在0.30...开展水槽试验获得铜合金编织网网片的阻力,以分析铜合金编织网网衣的阻力变化规律及水动力性能。试验制作了10片0.25 m^(2)不同铜丝直径及不同网目尺寸的铜合金编织网网片,于拖车静水槽中采用三分力传感器连接网片以测试网片受力,在0.30、0.45、0.60、0.75和0.85 m/s 5个拖车速度下开展水槽试验,每片网片在不同流速下都测得了0°~90°冲角范围,以10°间隔为节点共计10个冲角的阻力数据。结果显示:网片的阻力随流速的增加而增大,随着迎流冲角的增大,网片的阻力也在增加;相同网线直径的网片在特定流速与冲角下,其阻力随网目尺寸的增加而减小;相同网目尺寸的网片在特定流速与冲角下,其阻力随网线直径的增加而增大。基于网片试验数据,利用回归分析法得出铜合金编织网网衣在垂直流速时随流速变化的单位面积网衣阻力计算公式;利用点状图回归分析法得出铜合金编织网在垂直流速与平行流速两种状态下的阻力系数和雷诺数的对应关系;参考经验公式提出不同冲角下阻力系数的推导公式,并基于试验数据给出网片平面垂直于流速及平行于流速状态下的阻力系数,分别为1.20和0.21。本研究可为铜合金编织网网衣的受力计算以及水动力性能分析提供参考。展开更多
基金Supported by the Sub-project under National Science&Technology Support Plan(No.2013BAD13B03)the Special Fund for Agroscientific Research in the Public Interest(No.201203018)
文摘MicroRNAs(miRNAs) are an abundant class of conserved, non-coding small RNAs that play important role in gene regulation at post translational level. There have been no reports on the miRNAs of the Antarctic krill E uphausia superba despite the species' crucial position in Antarctic food webs. Two small RNA libraries were constructed from eyestalk and muscle, subsequently, and deep sequencing analysis was performed to investigate and profile E. superba miRNAs. A total of 19 304 586 and 23 005 104 unique sequences were obtained from the eyestalk and muscle, respectively. After compared the small RNA sequences with the Rfam database, 12 342 039 and 7 907 477 reads in eyestalk and muscle were matched to the transcriptome sequence of E. superba. A total of 236 distinct miRNAs were identified after annotation to known animal miRNAs registered in miRBase 21. In both libraries, the most abundant known miRNA were miR-750 with 92 583 reads in muscle and miR-1304-3 p with 56 386 reads in eyestalk while the average value was less than 106, revealing a wide range of different expression levels. In addition, miR-277 a enriched in both libraries and may be involved in modulating Krebs cycle by targeting to Vimar. Differential expression analysis showed that 56 mature miRNAs were significantly up/down regulated according to expression fold change. It appeared that the expression of several abundant miRNAs maybe tissue-specific or tissue-bias. Notably, the expression pattern of miR-750 and miR-1 family, which was suggested as the crucial candidates, involved in muscle development. Taken together, this study provides the first miRNA profile of E. superba and some of these miRNAs were expected to play important role in immune response, reproduction, energy metabolism, and muscle development and so on. Thus, the results provides a reference for functional studies of miRNAs in E. superba.
文摘目的利用文献计量学方法分析国内外治疗药物监测领域热点和趋势,结合复旦大学附属中山医院厦门医院(本院)2022年开展的治疗药物监测情况进行回顾性分析,为促进临床合理用药提供参考。方法基于Web of Science核心数据库和中国知网(CNKI)使用CiteSpace软件对2019—2023年关于治疗药物监测领域的研究进行关键词的共现分析、聚类分析。结果筛选后纳入1301篇英文文献、330篇中文文献进行文献计量学分析。国内外研究中的高频关键词包括治疗药物监测的药物以及分析方法。2022年本院开展药物浓度检测总例数为864例。监测排名前三位的药物分别是他克莫司(37.27%)、雷帕霉素(18.17%)和万古霉素(14.35%)。他克莫司的治疗药物浓度结果处于有效浓度范围占比最高为90.06%,其次是雷帕霉素为85.35%,地高辛为85.00%。结论目前治疗药物监测研究主要为万古霉素、地高辛、抗癫痫药物的合理用药。本院搭建临床药师参与报告解读的治疗药物监测平台,还需关注抗精神类药物以及抗肿瘤药物的治疗药物监测。
文摘开展水槽试验获得铜合金编织网网片的阻力,以分析铜合金编织网网衣的阻力变化规律及水动力性能。试验制作了10片0.25 m^(2)不同铜丝直径及不同网目尺寸的铜合金编织网网片,于拖车静水槽中采用三分力传感器连接网片以测试网片受力,在0.30、0.45、0.60、0.75和0.85 m/s 5个拖车速度下开展水槽试验,每片网片在不同流速下都测得了0°~90°冲角范围,以10°间隔为节点共计10个冲角的阻力数据。结果显示:网片的阻力随流速的增加而增大,随着迎流冲角的增大,网片的阻力也在增加;相同网线直径的网片在特定流速与冲角下,其阻力随网目尺寸的增加而减小;相同网目尺寸的网片在特定流速与冲角下,其阻力随网线直径的增加而增大。基于网片试验数据,利用回归分析法得出铜合金编织网网衣在垂直流速时随流速变化的单位面积网衣阻力计算公式;利用点状图回归分析法得出铜合金编织网在垂直流速与平行流速两种状态下的阻力系数和雷诺数的对应关系;参考经验公式提出不同冲角下阻力系数的推导公式,并基于试验数据给出网片平面垂直于流速及平行于流速状态下的阻力系数,分别为1.20和0.21。本研究可为铜合金编织网网衣的受力计算以及水动力性能分析提供参考。
