目的分析子痫前期患者血浆D-二聚体、C-反应蛋白(CRP)及凝血四项水平,探讨上述指标与子痫前期的相关性,为子痫前期的临床诊断及严重程度的评估提供参考。方法回顾并分析2019年1月—2021年9月徐州市妇幼保健院收治的257例孕妇的临床资料...目的分析子痫前期患者血浆D-二聚体、C-反应蛋白(CRP)及凝血四项水平,探讨上述指标与子痫前期的相关性,为子痫前期的临床诊断及严重程度的评估提供参考。方法回顾并分析2019年1月—2021年9月徐州市妇幼保健院收治的257例孕妇的临床资料,包括117名正常妊娠孕妇,66例轻度子痫前期孕妇以及74例重度子痫前期孕妇。检测所有孕妇血浆D-二聚体、CRP及凝血四项〔凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(Fib)、凝血酶时间(TT)〕;采用二元Logistic回归分析校验D-二聚体及CRP水平与疾病严重程度的相关性;采用Spearman相关性分析检验D-二聚体与CRP、新生儿出生体质量、1 min及5 min Apgar评分的相关性;绘制受试者工作特征曲线(ROC曲线)并计算ROC曲线下面积(AUC),评估D-二聚体对重度子痫前期的诊断价值。结果子痫前期孕妇的PT明显少于正常妊娠孕妇,TT、CRP水平均明显高于正常妊娠孕妇〔PT(s):10.94±0.84比11.66±0.55,TT(s):16.54±1.62比15.81±0.84,CRP(mg/L):12.35±8.01比6.06±5.52,均P<0.05〕。重度子痫前期孕妇血浆中D-二聚体水平相比轻度子痫前期者明显增高(mg/L:2.98±1.97比2.27±1.56,P<0.05),CRP水平较轻度子痫前期者稍高,但差异无统计学意义(mg/L:14.96±11.81比9.74±8.13,P>0.05)。Spearman相关性分析显示,子痫前期患者血浆D-二聚体与CRP呈正相关(r=0.225,P=0.008);二元Logistic回归分析结果表明,D-二聚体与子痫前期病情严重程度呈正相关〔优势比(OR)为1.264,95%可信区间(95%CI)为1.028~1.553,P=0.026〕;ROC曲线分析显示在截断值为2.12 mg/L时,D-二聚体对重度子痫前期诊断的AUC为0.595(95%CI为0.512~0.677),敏感度为63.5%,特异度为58.5%。结论孕晚期子痫前期孕妇存在凝血-纤溶功能失衡,血浆中D-二聚体水平与子痫前期病情严重程度呈正相关,且子痫前期孕妇D-二聚体与CRP水平呈正相关,应定期对子痫前期孕妇进行凝血-纤溶功能检测,给予合理的临床管理和干预。展开更多
【目的】研究陆地棉类受体激酶GhCRPK1(Cold-responsive protein kinase 1,CRPK1)在棉花纤维发育中的功能。【方法】以陆地棉全基因组关联分析(Genome-wide association study,GWAS)数据及陆地棉纤维发育不同时期的表达数据,筛选到GhCR...【目的】研究陆地棉类受体激酶GhCRPK1(Cold-responsive protein kinase 1,CRPK1)在棉花纤维发育中的功能。【方法】以陆地棉全基因组关联分析(Genome-wide association study,GWAS)数据及陆地棉纤维发育不同时期的表达数据,筛选到GhCRPK1在棉花纤维起始期优势表达,通过基因克隆、亚细胞定位等方法研究了GhCRPK1的功能,对过表达GhCRPK1的拟南芥及突变体crpk1的表皮毛长度和密度进行观察统计,利用酵母双杂交筛选与GhCRPK1相互作用的蛋白。【结果】GhCRPK1基因全长为4594 bp,开放阅读框长度为1047 bp,编码348个氨基酸,在开花当天到开花后3 d持续优势表达,编码产物定位在细胞膜上。GhCRPK1在拟南芥中异源表达后可促进表皮毛的伸长;拟南芥中同源基因突变后,表皮毛变短。酵母双杂交实验发现,GhCRPK1与热激蛋白Gh_D08G0057相互作用。【结论】GhCRPK1在棉花纤维发育起始时期优势表达,定位在细胞膜上,在拟南芥中异源表达后调控拟南芥表皮毛的伸长。展开更多
Background:Cotton is the source of natural fibers globally,fulfilling 90%of the textile industry’s requirements.However,fiber development is a complex biological process comprising four stages.Fiber develops from a s...Background:Cotton is the source of natural fibers globally,fulfilling 90%of the textile industry’s requirements.However,fiber development is a complex biological process comprising four stages.Fiber develops from a single cell,and cell elongation is a vital process in fiber development.Therefore,it is pertinent to understand and exploit mechanisms underlying cell elongation during fiber development.A previous report about cell division control protein 42(CDC-42)with its key role in cell elongation in eukaryotes inspired us to explore its homologs Rho GTPases for understanding of cell elongation during cotton fiber development.Result:We classified 2066 Rho proteins from 8 Gossypium species into 5 and 8 groups within A and D sub-genomes,respectively.Asymmetric evolution of Rho members was observed among five tetraploids.Population fixation statistics between two short and long fiber genotypes identified highly diverged regions encompassing 34 Rho genes in G.hirustum,and 31 of them were retained through further validation by genome wide association analysis(GWAS).Moreover,a weighted gene co-expression network characterized genome-wide expression patteren of Rho genes based on previously published transcriptome data.Twenty Rho genes from five modules were identified as hub genes which were potentially related to fiber development.Interaction networks of 5 Rho genes based on transcriptional abundance and gene ontology(GO)enrichment emphasized the involvement of Rho in cell wall biosynthesis,fatty acid elongation,and other biological processes.Conclusion:Our study characterized the Rho proteins in cotton,provided insights into the cell elongation of cotton fiber and potential application in cotton fiber improvement.展开更多
Introduction:Genome sequence plays an important role in both basic and applied studies.Gossypium raimondii,the putative contributor of the D subgenome of upland cotton(G.hirsutum,highlights the need to improve the gen...Introduction:Genome sequence plays an important role in both basic and applied studies.Gossypium raimondii,the putative contributor of the D subgenome of upland cotton(G.hirsutum,highlights the need to improve the genome quality rapidly and efficiently.Methods:We performed Hi-C sequencing of G.raimondii and reassembled its genome based on a set of new Hi-C data and previously published scaffolds.We also compared the reassembled genome sequenee with the previously published G raimondii genomes for gene and genome sequence collinearity.Result:A total of 9842%of scaffold sequences were clustered successfully,among which 99.72%of the clustered sequences were ordered and 99.92%of the ordered sequences were oriented with high-quality.Further evaluation of results by heat-map and collinearity analysis revealed that the current reassembled genome is significantly improved than the previous one(Nat Genet 44:98-1103,2012).Conclusion:This improvement in G raimondii genome not only provides a better reference to increase study efficiency but also offers a new way to assemble cotton genomes.Furthermore,Hi-C data of G.raimondii may be used for 3D structure research or regulating analysis.展开更多
Background： Virescence, as a recognizable phenotype available for research on chloroplast development and in the early development stage of cotton, is not only photosynthesis but also for heterosis exploitation in co...Background： Virescence, as a recognizable phenotype available for research on chloroplast development and in the early development stage of cotton, is not only photosynthesis but also for heterosis exploitation in cotton Methods： In current study, for fine mapping of virescent-1 （V1） in cotton, three populations with a total of 5 678 individuals were constructed using T582 which has the virescent trait. Tobacco rattle virus, TRV1 and TRV2 （pYL156）, were used as vectors for the virus-induced gene silencing （VlGS） assay. Results： The V1 gene was fine-mapped to a 20 kb interval on chromosome 20 of tetraploid cotton. We identified only one candidate gene with four single nucleotide polymorphisms between parents, among which the single nucleotide polymorphism at the position of 1 082 base pair caused the change of amino acid residue from Arg （3-79） to Lys （]-582）. The relative expression of the candidate gene in virescent plants was extensively lower than that in normal plants. Nullification of the gene by VlGS significantly turned the green leaf of normal cotton plants into yellow. We named this candidate gene as GhRVL. Conclusions： This study will facilitate the further research on virescent formation, and will be useful for breeding of hybrid cottons.展开更多
文摘目的分析子痫前期患者血浆D-二聚体、C-反应蛋白(CRP)及凝血四项水平,探讨上述指标与子痫前期的相关性,为子痫前期的临床诊断及严重程度的评估提供参考。方法回顾并分析2019年1月—2021年9月徐州市妇幼保健院收治的257例孕妇的临床资料,包括117名正常妊娠孕妇,66例轻度子痫前期孕妇以及74例重度子痫前期孕妇。检测所有孕妇血浆D-二聚体、CRP及凝血四项〔凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(Fib)、凝血酶时间(TT)〕;采用二元Logistic回归分析校验D-二聚体及CRP水平与疾病严重程度的相关性;采用Spearman相关性分析检验D-二聚体与CRP、新生儿出生体质量、1 min及5 min Apgar评分的相关性;绘制受试者工作特征曲线(ROC曲线)并计算ROC曲线下面积(AUC),评估D-二聚体对重度子痫前期的诊断价值。结果子痫前期孕妇的PT明显少于正常妊娠孕妇,TT、CRP水平均明显高于正常妊娠孕妇〔PT(s):10.94±0.84比11.66±0.55,TT(s):16.54±1.62比15.81±0.84,CRP(mg/L):12.35±8.01比6.06±5.52,均P<0.05〕。重度子痫前期孕妇血浆中D-二聚体水平相比轻度子痫前期者明显增高(mg/L:2.98±1.97比2.27±1.56,P<0.05),CRP水平较轻度子痫前期者稍高,但差异无统计学意义(mg/L:14.96±11.81比9.74±8.13,P>0.05)。Spearman相关性分析显示,子痫前期患者血浆D-二聚体与CRP呈正相关(r=0.225,P=0.008);二元Logistic回归分析结果表明,D-二聚体与子痫前期病情严重程度呈正相关〔优势比(OR)为1.264,95%可信区间(95%CI)为1.028~1.553,P=0.026〕;ROC曲线分析显示在截断值为2.12 mg/L时,D-二聚体对重度子痫前期诊断的AUC为0.595(95%CI为0.512~0.677),敏感度为63.5%,特异度为58.5%。结论孕晚期子痫前期孕妇存在凝血-纤溶功能失衡,血浆中D-二聚体水平与子痫前期病情严重程度呈正相关,且子痫前期孕妇D-二聚体与CRP水平呈正相关,应定期对子痫前期孕妇进行凝血-纤溶功能检测,给予合理的临床管理和干预。
文摘【目的】研究陆地棉类受体激酶GhCRPK1(Cold-responsive protein kinase 1,CRPK1)在棉花纤维发育中的功能。【方法】以陆地棉全基因组关联分析(Genome-wide association study,GWAS)数据及陆地棉纤维发育不同时期的表达数据,筛选到GhCRPK1在棉花纤维起始期优势表达,通过基因克隆、亚细胞定位等方法研究了GhCRPK1的功能,对过表达GhCRPK1的拟南芥及突变体crpk1的表皮毛长度和密度进行观察统计,利用酵母双杂交筛选与GhCRPK1相互作用的蛋白。【结果】GhCRPK1基因全长为4594 bp,开放阅读框长度为1047 bp,编码348个氨基酸,在开花当天到开花后3 d持续优势表达,编码产物定位在细胞膜上。GhCRPK1在拟南芥中异源表达后可促进表皮毛的伸长;拟南芥中同源基因突变后,表皮毛变短。酵母双杂交实验发现,GhCRPK1与热激蛋白Gh_D08G0057相互作用。【结论】GhCRPK1在棉花纤维发育起始时期优势表达,定位在细胞膜上,在拟南芥中异源表达后调控拟南芥表皮毛的伸长。
基金supported by Funds of the National Key Research and Development Program(2016YFD0101006,2018YFD0100402)National Natural Science Foundation of China(31621005 and 31901581)Central Public-interest Scientific Institution Basal Research Fund(1610162021013).
文摘Background:Cotton is the source of natural fibers globally,fulfilling 90%of the textile industry’s requirements.However,fiber development is a complex biological process comprising four stages.Fiber develops from a single cell,and cell elongation is a vital process in fiber development.Therefore,it is pertinent to understand and exploit mechanisms underlying cell elongation during fiber development.A previous report about cell division control protein 42(CDC-42)with its key role in cell elongation in eukaryotes inspired us to explore its homologs Rho GTPases for understanding of cell elongation during cotton fiber development.Result:We classified 2066 Rho proteins from 8 Gossypium species into 5 and 8 groups within A and D sub-genomes,respectively.Asymmetric evolution of Rho members was observed among five tetraploids.Population fixation statistics between two short and long fiber genotypes identified highly diverged regions encompassing 34 Rho genes in G.hirustum,and 31 of them were retained through further validation by genome wide association analysis(GWAS).Moreover,a weighted gene co-expression network characterized genome-wide expression patteren of Rho genes based on previously published transcriptome data.Twenty Rho genes from five modules were identified as hub genes which were potentially related to fiber development.Interaction networks of 5 Rho genes based on transcriptional abundance and gene ontology(GO)enrichment emphasized the involvement of Rho in cell wall biosynthesis,fatty acid elongation,and other biological processes.Conclusion:Our study characterized the Rho proteins in cotton,provided insights into the cell elongation of cotton fiber and potential application in cotton fiber improvement.
文摘Introduction:Genome sequence plays an important role in both basic and applied studies.Gossypium raimondii,the putative contributor of the D subgenome of upland cotton(G.hirsutum,highlights the need to improve the genome quality rapidly and efficiently.Methods:We performed Hi-C sequencing of G.raimondii and reassembled its genome based on a set of new Hi-C data and previously published scaffolds.We also compared the reassembled genome sequenee with the previously published G raimondii genomes for gene and genome sequence collinearity.Result:A total of 9842%of scaffold sequences were clustered successfully,among which 99.72%of the clustered sequences were ordered and 99.92%of the ordered sequences were oriented with high-quality.Further evaluation of results by heat-map and collinearity analysis revealed that the current reassembled genome is significantly improved than the previous one(Nat Genet 44:98-1103,2012).Conclusion:This improvement in G raimondii genome not only provides a better reference to increase study efficiency but also offers a new way to assemble cotton genomes.Furthermore,Hi-C data of G.raimondii may be used for 3D structure research or regulating analysis.
基金The National Key Research and Development Program of China(2016YFD0101401)
文摘Background： Virescence, as a recognizable phenotype available for research on chloroplast development and in the early development stage of cotton, is not only photosynthesis but also for heterosis exploitation in cotton Methods： In current study, for fine mapping of virescent-1 （V1） in cotton, three populations with a total of 5 678 individuals were constructed using T582 which has the virescent trait. Tobacco rattle virus, TRV1 and TRV2 （pYL156）, were used as vectors for the virus-induced gene silencing （VlGS） assay. Results： The V1 gene was fine-mapped to a 20 kb interval on chromosome 20 of tetraploid cotton. We identified only one candidate gene with four single nucleotide polymorphisms between parents, among which the single nucleotide polymorphism at the position of 1 082 base pair caused the change of amino acid residue from Arg （3-79） to Lys （]-582）. The relative expression of the candidate gene in virescent plants was extensively lower than that in normal plants. Nullification of the gene by VlGS significantly turned the green leaf of normal cotton plants into yellow. We named this candidate gene as GhRVL. Conclusions： This study will facilitate the further research on virescent formation, and will be useful for breeding of hybrid cottons.
