This study aims to establish the occupational exposure limit （OEL） in the air for workplace of warfarin based on the available toxicological studies and field investigations by using questionnaire and air monitoring...This study aims to establish the occupational exposure limit （OEL） in the air for workplace of warfarin based on the available toxicological studies and field investigations by using questionnaire and air monitoring. The clinical therapeutic dose was used as lowest observed effect level （LOEL）, and no observed effect level （NOEL） was achieved by using a safety factor. The highest concentration of warfarin monitored in the worksite of centrifuge washing, drying and packing were 0.029 mg/m3, 0.052 mg/m3 respectively, which did not exceed the OEL 0.2 mg/m3 recommended by NIOSH and ACGIH. Considering its feasibility for enforcement and protection for workers, we recommend OEL 0.2 mg/m3 of warfarin in China.展开更多
Objective To establish the model of human bronchial epithelial cells （16HBE） malignant transformation induced by glycidyl methacrylate （GMA） and define the different methylation genes at different stages. Methods ...Objective To establish the model of human bronchial epithelial cells （16HBE） malignant transformation induced by glycidyl methacrylate （GMA） and define the different methylation genes at different stages. Methods DNA was extracted at different 16HBE malignant phases and methylation at different stages were detected using Methylation chip of Promoter Microarray Methylation＇. Methylation-specific PCR （MSP） was methylation status of some genes, and then compared with the control groups. changes of genes DNA ＇NimbleGen HG18 CpG used to observe the Results The result showed that GMA induced 16HBE morphorlogical transformation at the dose of 8 I^g/mL, and cell exposed to GMA had 1 374 genes in protophase, 825 genes in metaphase, 1 149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not in metaphase and anaphase; 272 genes in metaphase but not in protophase and anaphase; 683 genes in anaphase but not in metaphase and protophase; 73 genes in protophase and metaphase but not in anaphase; 67 genes in protophase and anaphase but not in metaphase; 59 genes in metaphase and anaphase but not in protophase. Conclusion The pattern of DNA methylation could change in the process of 16HBE induced by GMA.展开更多
基金supported by the Occupational Health Standards Program (20100304) from Ministry of Health of China
文摘This study aims to establish the occupational exposure limit （OEL） in the air for workplace of warfarin based on the available toxicological studies and field investigations by using questionnaire and air monitoring. The clinical therapeutic dose was used as lowest observed effect level （LOEL）, and no observed effect level （NOEL） was achieved by using a safety factor. The highest concentration of warfarin monitored in the worksite of centrifuge washing, drying and packing were 0.029 mg/m3, 0.052 mg/m3 respectively, which did not exceed the OEL 0.2 mg/m3 recommended by NIOSH and ACGIH. Considering its feasibility for enforcement and protection for workers, we recommend OEL 0.2 mg/m3 of warfarin in China.
基金supported by National Natural Science Foundation of China(81072318)
文摘Objective To establish the model of human bronchial epithelial cells （16HBE） malignant transformation induced by glycidyl methacrylate （GMA） and define the different methylation genes at different stages. Methods DNA was extracted at different 16HBE malignant phases and methylation at different stages were detected using Methylation chip of Promoter Microarray Methylation＇. Methylation-specific PCR （MSP） was methylation status of some genes, and then compared with the control groups. changes of genes DNA ＇NimbleGen HG18 CpG used to observe the Results The result showed that GMA induced 16HBE morphorlogical transformation at the dose of 8 I^g/mL, and cell exposed to GMA had 1 374 genes in protophase, 825 genes in metaphase, 1 149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not in metaphase and anaphase; 272 genes in metaphase but not in protophase and anaphase; 683 genes in anaphase but not in metaphase and protophase; 73 genes in protophase and metaphase but not in anaphase; 67 genes in protophase and anaphase but not in metaphase; 59 genes in metaphase and anaphase but not in protophase. Conclusion The pattern of DNA methylation could change in the process of 16HBE induced by GMA.
